Laboratoř bioanalýzy a zobrazování

@article{pmid26046318,

title = {Fabrication of solid-state nanopores and its perspectives},

author = {Jiri Kudr and Sylvie Skalickova and Lukas Nejdl and Amitava Moulick and Branislav Ruttkay-Nedecky and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201400612},

issn = {1522-2683},

year  = {2015},

date = {2015-10-01},

journal = {Electrophoresis},

volume = {36},

number = {19},

pages = {2367--2379},

abstract = {Nanofluidics is becoming an extensively developing technique in the field of bioanalytical chemistry. Nanoscale hole embed in an insulating membrane is employed in a vast variety of sensing platforms and applications. Although, biological nanopores have several attractive characteristics, in this paper, we focused on the solid-state nanopores due to their advantages as high stability, possibility of diameter control, and ease of surface functionalizing. A detection method, based on the translocation of analyzed molecules through nanochannels under applied voltage bias and resistive pulse sensing, is well established. Nevertheless, it seems that the new detection methods like measuring of transverse electron tunneling using nanogap electrodes or optical detection can offer significant additional advantages. The aim of this review is not to cite all related articles, but highlight the steps, which in our opinion, meant important progresses in solid-state nanopore analysis. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26209776,

title = {Structural effects and nanoparticle size are essential for quantum dots-metallothionein complex formation},

author = {Katerina Tmejova and David Hynek and Pavel Kopel and Jaromir Gumulec and Sona Krizkova and Roman Guran and Zbynek Heger and Michal Kalina and Marketa Vaculovicova and Vojtech Adam and Rene Kizek},

doi = {10.1016/j.colsurfb.2015.06.045},

issn = {1873-4367},

year  = {2015},

date = {2015-10-01},

journal = {Colloids Surf B Biointerfaces},

volume = {134},

pages = {262--272},

abstract = {Interaction between semiconductor nanocrystals, cadmium telluride quantum dots (CdTe QDs) capped with mercaptosuccinic acid (MSA) and metallothionein (MT) was investigated. MSA-capped CdTe QDs were synthesized in aqueous solution. Mixture of MT and MSA-capped CdTe QDs has been investigated by various analytical methods as follows: tris-tricine gel electrophoresis, fluorescence evaluation and electrochemical detection of catalysed hydrogen evolution. The obtained results demonstrate that MSA-capped CdTe QDs and MT do not create firmly bound stabile complex. However, weak electrostatic interactions contribute to the interaction of MT with MSA-capped CdTeQDs. It can be concluded that QDs size influences the QDs and MT interaction. The smallest QDs had the highest affinity to MT and vice versa. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26033737,

title = {Label-free bead-based metallothionein electrochemical immunosensor},

author = {Lukas Nejdl and Hoai Viet Nguyen and Lukas Richtera and Sona Krizkova and Roman Guran and Michal Masarik and David Hynek and Zbynek Heger and Karin Lundberg and Kristofer Erikson and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201500069},

issn = {1522-2683},

year  = {2015},

date = {2015-08-01},

journal = {Electrophoresis},

volume = {36},

number = {16},

pages = {1894--1904},

abstract = {A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. The assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 μL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 μg/mL) in samples of patients with cancer. The stability of constructed device for metallothionein detection in flow arrangement was found to be several days without any maintenance needed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25808901,

title = {Doxorubicin interactions with bovine serum albumin revealed by microdialysis with on-line laser-induced fluorescence detection at subpicogram level},

author = {Iva Blazkova and Kristyna Smerkova and Lucie Blazkova and Marketa Vaculovicova and Marie Stiborova and Tomas Eckschlager and Miroslava Beklova and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201400564},

issn = {1522-2683},

year  = {2015},

date = {2015-06-01},

journal = {Electrophoresis},

volume = {36},

number = {11-12},

pages = {1282--1288},

abstract = {Doxorubicin (DOX) is an effective antitumor drug employed for treatment of a wide range of cancers types such as neuroblastoma, osteosarcoma, breast and esophageal carcinomas. On the other hand, the cumulative dose is restricted (300-550 mg/m(2)) and its amount administered to a patient has to be closely controlled due to its cardiotoxicity. To understand the mechanisms of the DOX side effects as well as to reveal the ways how to reduce its adverse impact on cardiomyocytes, the interactions with particular components of the blood and tissues have to be studied in greater detail. In this work, microdialysis technique was optimized to extract DOX from samples and subsequently monitor its interaction with BSA. Finally, the microdialysis probe was connected on-line to the LIF detector to ensure the real-time detection. The best flow rate was 1 μL/min and after 120 min of microdialysis 28% of the DOX was dialyzed out from the sample. The results from investigation of the DOX-BSA interaction indicate that the interaction occurs in less than 30 min, causing marked decrease in the amount of DOX extracted by microdialysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25847165,

title = {Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems},

author = {Markéta Vaculovičová and Mahbub Akther and Pleun Maaskant and Dermot Brabazon and Mirek Macka},

doi = {10.1016/j.aca.2015.02.044},

issn = {1873-4324},

year  = {2015},

date = {2015-04-01},

journal = {Anal Chim Acta},

volume = {871},

pages = {85--92},

abstract = {In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro-light emitting diode (μ-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN μ-LED array consisted of 270 individual μ-LED elements with a peak emission at 470 nm, each about 14 μm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (OF, 400 μm in diameter, numerical aperture NA=0.37), to form an integrated μ-LED-IF-OF light source component. This packaged μ-LED-IF-OF light source emitted approximately 225 μW of optical power at a bias current of 20 mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm. Devices with and without IF were compared in terms of the optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers, it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1×10(-8) mol L(-1) obtained under optimised conditions. The μ-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. The limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25847165b,

title = {Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems},

author = {Markéta Vaculovičová and Mahbub Akther and Pleun Maaskant and Dermot Brabazon and Mirek Macka},

doi = {10.1016/j.aca.2015.02.044},

issn = {1873-4324},

year  = {2015},

date = {2015-04-01},

journal = {Anal Chim Acta},

volume = {871},

pages = {85--92},

abstract = {In this work, a new type of miniaturized fibre-coupled solid-state light source is demonstrated as an excitation source for fluorescence detection in capillary electrophoresis. It is based on a parabolically shaped micro-light emitting diode (μ-LED) array with a custom band-pass optical interference filter (IF) deposited at the back of the LED substrate. The GaN μ-LED array consisted of 270 individual μ-LED elements with a peak emission at 470 nm, each about 14 μm in diameter and operated as a single unit. Light was extracted through the transparent substrate material, and coupled to an optical fibre (OF, 400 μm in diameter, numerical aperture NA=0.37), to form an integrated μ-LED-IF-OF light source component. This packaged μ-LED-IF-OF light source emitted approximately 225 μW of optical power at a bias current of 20 mA. The bandpass IF filter was designed to reduce undesirable LED light emissions in the wavelength range above 490 nm. Devices with and without IF were compared in terms of the optical power output, spectral characteristics as well as LOD values. While the IF consisted of only 7.5 pairs (15 layers) of SiO2/HfO2 layers, it resulted in an improvement of the baseline noise as well as the detection limit measured using fluorescein as test analyte, both by approximately one order of magnitude, with a LOD of 1×10(-8) mol L(-1) obtained under optimised conditions. The μ-LED-IF-OF light source was then demonstrated for use in capillary electrophoresis with fluorimetric detection. The limits of detection obtained by this device were compared to those obtained with a commercial fibre coupled LED device.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25837469,

title = {Study of linkage between glutathione pathway and the antibiotic resistance of Escherichia coli from patients' swabs},

author = {Marketa Kominkova and Petr Michalek and Kristyna Cihalova and Roman Guran and Natalia Cernei and Lukas Nejdl and Kristyna Smerkova and Simona Dostalova and Dagmar Chudobova and Zbynek Heger and Radek Vesely and Jaromir Gumulec and Jindrich Kynicky and Kledi Xhaxhiu and Ondrej Zitka and Vojtech Adam and Rene Kizek},

doi = {10.3390/ijms16047210},

issn = {1422-0067},

year  = {2015},

date = {2015-03-01},

journal = {Int J Mol Sci},

volume = {16},

number = {4},

pages = {7210--7229},

abstract = {In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase-GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of "more resistant" E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32262379,

title = {Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles},

author = {Jiri Kudr and Lukas Nejdl and Sylvie Skalickova and Michal Zurek and Vedran Milosavljevic and Renata Kensova and Branislav Ruttkay-Nedecky and Pavel Kopel and David Hynek and Marie Novotna and Vojtech Adam and Rene Kizek},

doi = {10.1039/c4tb01336k},

issn = {2050-7518},

year  = {2015},

date = {2015-03-01},

journal = {J Mater Chem B},

volume = {3},

number = {10},

pages = {2109--2118},

abstract = {The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 °C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles via oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25405852,

title = {Label-free and amplification-free miR-124 detection in human cells},

author = {Kristyna Smerkova and Kristyna Hudcova and Veronika Vlahova and Marketa Vaculovicova and Vladimir Pekarik and Michal Masarik and Vojtech Adam and Rene Kizek},

doi = {10.3892/ijo.2014.2756},

issn = {1791-2423},

year  = {2015},

date = {2015-02-01},

journal = {Int J Oncol},

volume = {46},

number = {2},

pages = {871--877},

abstract = {MicroRNAs (miRNAs) are becoming a very important group of molecules especially since their connection to numerous diseases has been revealed. The potential in gene therapy as well as in diagnostics is being widely investigated leading to the demand of sensitive, selective and simple methods of isolation and detection. The combined advantages of magnetic particle-based separation with sensitive electrochemical detection may offer a very valuable tool for these purposes. In this study, the miR‑124 was targeted as an example analyte for development and optimization of the isolation procedure coupled to the electrochemical detection. The sensitivity of the method was demonstrated by the limit of detection at the level of nanomolar concentration (4 nM). To verify the applicability of the procedure to the real samples, miR‑124 was isolated from the human embryonic kidney cells naturally expressing this miRNA molecule and the results were compared to the amount of miR‑124 isolated from the cells transfected by the pENTR-miR‑124 plasmid leading to the overexpression of miR‑124.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25218889,

title = {Interaction study of arsenic (III and V) ions with metallothionein gene (MT2A) fragment},

author = {Lukas Nejdl and Sylvie Skalickova and Jiri Kudr and Branislav Ruttkay-Nedecky and Simona Dostalova and Monika Kremplova and Renata Kensova and Amitava Moulick and Marie Konecna and Vojtech Adam and Rene Kizek},

doi = {10.1016/j.ijbiomac.2014.09.001},

issn = {1879-0003},

year  = {2015},

date = {2015-01-01},

journal = {Int J Biol Macromol},

volume = {72},

pages = {599--605},

abstract = {Arsenic compounds belong to the most controversial agents concerning human health. Arsenic (As) is considered as a top environmental element influencing human health due to its adverse effects including cancer, diabetes, cardiovascular disease, and reproductive or developmental problems. Despite the proven mutagenic, teratogenic and carcinogenic effects, the arsenic compounds are used for centuries to treat infectious diseases. In our work, we focused on studying of interactions of As(III) and/or As(V) with DNA. Interactions between arsenic ions and DNA were monitored by UV/vis spectrophotometry by measuring absorption and fluorescence spectra, atomic absorption spectrometry, electrochemical measurements (square wave voltammetry) and agarose gel electrophoresis. Using these methods, we observed a stable structure of DNA with As(III) within the concentration range 0.4-6.25 μg mL(-1). Higher As(III) concentration caused degradation of DNA. However, similar effects were not observed for As(V).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25558996,

title = {Simultaneous automatic electrochemical detection of zinc, cadmium, copper and lead ions in environmental samples using a thin-film mercury electrode and an artificial neural network},

author = {Jiri Kudr and Hoai Viet Nguyen and Jaromir Gumulec and Lukas Nejdl and Iva Blazkova and Branislav Ruttkay-Nedecky and David Hynek and Jindrich Kynicky and Vojtech Adam and Rene Kizek},

doi = {10.3390/s150100592},

issn = {1424-8220},

year  = {2014},

date = {2014-12-01},

journal = {Sensors (Basel)},

volume = {15},

number = {1},

pages = {592--610},

abstract = {In this study a device for automatic electrochemical analysis was designed. A three electrodes detection system was attached to a positioning device, which enabled us to move the electrode system from one well to another of a microtitre plate. Disposable carbon tip electrodes were used for Cd(II), Cu(II) and Pb(II) ion quantification, while Zn(II) did not give signal in this electrode configuration. In order to detect all mentioned heavy metals simultaneously, thin-film mercury electrodes (TFME) were fabricated by electrodeposition of mercury on the surface of carbon tips. In comparison with bare electrodes the TMFEs had lower detection limits and better sensitivity. In addition to pure aqueous heavy metal solutions, the assay was also performed on mineralized rock samples, artificial blood plasma samples and samples of chicken embryo organs treated with cadmium. An artificial neural network was created to evaluate the concentrations of the mentioned heavy metals correctly in mixture samples and an excellent fit was observed (R2 = 0.9933). },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Interaction of E6 Gene from Human Papilloma Virus 16 (HPV-16) with CdS Quantum Dots},

author = {Lukas Nejdl and Sylvie Skalickova and Jiri Kudr and Branislav Ruttkay-Nedecky and Hoai Nguyen and Miguel Rodrigo and Pavel Kopel and Marie Konecna and Vojtech Adam and Rene Kizek},

url = {https://www.researchgate.net/publication/265842318_Interaction_of_E6_Gene_from_Human_Papilloma_Virus_16_HPV-16_with_CdS_Quantum_Dots},

doi = {10.1007/s10337-014-2734-5},

issn = { 0009-5893},

year  = {2014},

date = {2014-11-01},

urldate = {2014-11-01},

journal = {Chromatographia},

volume = {77},

pages = {1433-1439},

abstract = {The aim of this study was to analyze the interactions of blue and yellow fluorescent CdS quantum dots (CdS-QDs) with human papillomavirus 16 (HPV-16) oncogene E6. The interactions were investigated using chip capillary electrophoresis, spectrophotometry and square wave voltammetry (SWV). Using chip capillary electrophoresis we proved that blue fluorescent CdS-QDs (0.5 mM) caused an increase of the migration time of the E6 HPV-16 DNA–CdS-QDs complex by 42 s compared to control DNA (E6 HPV-16). The same concentration of yellow fluorescent CdS-QDs caused an increase in the migration time of the DNA–CdS-QDs complex by 108 s compared to the control DNA (E6 HPV-16). The difference in the migration times between both complexes was 66 s. Using square wave voltammetry (SWV), the reduction signal of cytosine and adenine (peak CA) was observed, after the complex with 2.5 µg mL−1 DNA was formed. A decrease of the peak CA reduction signal of the complex DNA–CdS-QDs by 90 % was caused when yellow fluorescent CdS-QDs (0.03 mM) were used. The same concentration of blue fluorescent CdS-QDs caused only a 50 % decrease of the C and A reduction signal of the DNA–CdS-QDs complex. The difference between both CdS-QDs was 40 %. Electrochemical measurements and chip electrophoresis analyses confirmed that the yellow fluorescent CdS-QDs show higher affinity to the DNA (E6 HPV-16) compared to blue ones.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser-Induced Fluorescence Detection},

author = {Romana Konecna and Hoai Nguyen and Maja Stanisavljevic and Sona Krizkova and Marketa Vaculovicova and Marie Stiborová and Tomáš Eckschlager and Ondrej Zitka and Vojtech Adam and Rene Kizek },

url = {https://www.researchgate.net/publication/272573039_Doxorubicin_Encapsulation_Investigated_by_Capillary_Electrophoresis_with_Laser-Induced_Fluorescence_Detection},

doi = {10.1007/s10337-014-2733-6},

issn = {0009-5893},

year  = {2014},

date = {2014-11-01},

urldate = {2014-11-01},

journal = {Chromatographia},

volume = {77},

pages = {1469-1476},

abstract = {Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4-10. Two times higher fluorescence intensity was observed at pH 4 compared to pH 10. Further, the DOX behavior in capillary electrophoresis (CE) was investigated. Electrophoretic mobilities (CE) in various pH of the background electrolyte were determined within the range from 16.3 to -13.3 x 10 (-9) m(-2) V-1 s(-1). Finally, CE was also used to monitor the encapsulation of DOX into the cavity of apoferritin as well as the pH-triggered release.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24981309,

title = {Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser-induced fluorescence detection},

author = {Maja Stanisavljevic and Jana Chomoucka and Simona Dostalova and Sona Krizkova and Marketa Vaculovicova and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201400204},

issn = {1522-2683},

year  = {2014},

date = {2014-09-01},

journal = {Electrophoresis},

volume = {35},

number = {18},

pages = {2587--2592},

abstract = {Quantum dots (QDs) are one of the most promising nanomaterials, due to their size-dependent characteristics as well as easily controllable size during the synthesis process. They are promising label material and their interaction with biomolecules is of great interest for science. In this study, CdTe QDs were synthesized under optimal conditions for 2 nm size. Characterization and verification of QDs synthesis procedure were done by fluorimetric method and with CE. Afterwards, QDs interaction with chicken genomic DNA and 500 bpDNA fragment was observed employing CE-LIF and gel electrophoresis. Performed interaction relies on possible matching between size of QDs and major groove of the DNA, which is approximately 2.1 nm.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24634313,

title = {Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment},

author = {Lukas Nejdl and Jiri Kudr and Kristyna Cihalova and Dagmar Chudobova and Michal Zurek and Ludek Zalud and Lukas Kopecny and Frantisek Burian and Branislav Ruttkay-Nedecky and Sona Krizkova and Marie Konecna and David Hynek and Pavel Kopel and Jan Prasek and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201300576},

issn = {1522-2683},

year  = {2014},

date = {2014-08-01},

journal = {Electrophoresis},

volume = {35},

number = {16},

pages = {2333--2345},

abstract = {Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 μL. The flow rate was optimized to 500 μL/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticles modified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per μL. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Plasmid HIV p24 Gene Detection on Mercury Film Electrode using Osmium Labelling},

author = {Jiri Kudr and Lukas Nejdl and Sylvie Skalickova and Branislav Ruttkay-Nedecky and Miguel Rodrigo and Simona Rex and Ana Maria Jimenez and Dagmar Chudobova and Kristyna Dolezelikova and Marie Konecna and Pavel Kopel and Jindrich Kynicky and Vojtech Adam and Rene Kizek},

url = {https://www.researchgate.net/publication/264055171_Plasmid_HIV_p24_Gene_Detection_on_Mercury_Film_Electrode_using_Osmium_Labelling},

issn = {1452-3981},

year  = {2014},

date = {2014-07-01},

urldate = {2014-07-01},

journal = {International Journal of Electrochemical Science},

volume = {9},

pages = {3409-3418},

abstract = {The early detection of HIV-positive individuals before infecting healthy individuals is the basic principle to fight against AIDS. Although a lot of methods has been developed to detect HIV, electrochemistry is a promising tool thanks to its high sensitivity, low cost, ease of use and mostly no antibody-based detection. The aim of our study was to perform the electrochemical detection of HIV p24 gene for capsid protein. We utilized the ability of osmium tetroxide bipyridyl (OsO4(bpy)) to create the complex with thymine and used it to label the oligonucleotide complementary to the p24. We also used the thiolated oligonucleotide probe to anchor the labelled p24 gene to the magnetic particle, which served to isolate the construct from the solution of non-anchored molecules. The detection of p24 gene was performed on the mercury film glassy-carbon electrode by the adsorptive transfer method using difference pulse voltammetry. The best mercury film on the surface of glassy carbon working electrode was received using KNO3 as supporting electrolyte and by the set of potential -0.9 V for 300 s. We showed that also the femtomole amount of p24 gene within linear plasmide can be absorbed to the electrode surface and detected.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24648211,

title = {Capillary electrophoresis of quantum dots: minireview},

author = {Maja Stanisavljevic and Marketa Vaculovicova and Rene Kizek and Vojtech Adam},

doi = {10.1002/elps.201400033},

issn = {1522-2683},

year  = {2014},

date = {2014-07-01},

journal = {Electrophoresis},

volume = {35},

number = {14},

pages = {1929--1937},

abstract = {It has been already three decades, since the fluorescent nanocrystals called quantum dots (QDs) appeared and attracted attention of a broad scientific community. Their excellent not only optical but also electronic properties predetermined QDs for utilization in a variety of areas. Besides lasers, solar cells, and/or computers, QDs have established themselves in the field of (bio)chemical labeling as well as medical imaging. However, due to the numerous application possibilities of QDs, there are high demands on their properties that need to be precisely controlled and characterized. CE with its versatile modes and possibilities of detection was found to be an effective tool not only for characterization of QDs size and/or surface properties but also for monitoring of their interactions with other molecules of interest. In this minireview, we are giving short insight in analysis of QDs by CE, and summarizing the advantages of this method for QDs characterization.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24648211b,

title = {Capillary electrophoresis of quantum dots: minireview},

author = {Maja Stanisavljevic and Marketa Vaculovicova and Rene Kizek and Vojtech Adam},

doi = {10.1002/elps.201400033},

issn = {1522-2683},

year  = {2014},

date = {2014-07-01},

journal = {Electrophoresis},

volume = {35},

number = {14},

pages = {1929--1937},

abstract = {It has been already three decades, since the fluorescent nanocrystals called quantum dots (QDs) appeared and attracted attention of a broad scientific community. Their excellent not only optical but also electronic properties predetermined QDs for utilization in a variety of areas. Besides lasers, solar cells, and/or computers, QDs have established themselves in the field of (bio)chemical labeling as well as medical imaging. However, due to the numerous application possibilities of QDs, there are high demands on their properties that need to be precisely controlled and characterized. CE with its versatile modes and possibilities of detection was found to be an effective tool not only for characterization of QDs size and/or surface properties but also for monitoring of their interactions with other molecules of interest. In this minireview, we are giving short insight in analysis of QDs by CE, and summarizing the advantages of this method for QDs characterization.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24296063,

title = {3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots},

author = {Ludmila Krejcova and Lukas Nejdl and Miguel Angel Merlos Rodrigo and Michal Zurek and Miroslav Matousek and David Hynek and Ondrej Zitka and Pavel Kopel and Vojtech Adam and Rene Kizek},

doi = {10.1016/j.bios.2013.10.031},

issn = {1873-4235},

year  = {2014},

date = {2014-04-01},

journal = {Biosens Bioelectron},

volume = {54},

pages = {421--427},

abstract = {In this study, we report a new three-dimensional (3D), bead-based microfluidic chip developed for rapid, sensitive and specific detection of influenza hemagglutinin. The principle of microfluidic chip is based on implementation of two-step procedure that includes isolation based on paramagnetic beads and electrochemical detection. As a platform for isolation process, streptavidin-modified MPs, which were conjugated via biotinylated glycan (through streptavidin-biotin affinity) followed by linkage of hemagglutinin to glycan, were used. Vaccine hemagglutinin (HA vaxi) was labeled with CdS quantum dots (QDs) at first. Detection of the isolation product by voltammetry was the end point of the procedure. The suggested and developed method can be used also for detection of other specific substances that are important for control, diagnosis or therapy of infectious diseases. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24651395,

title = {Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus},

author = {Dagmar Chudobova and Simona Dostalova and Iva Blazkova and Petr Michalek and Branislav Ruttkay-Nedecky and Matej Sklenar and Lukas Nejdl and Jiri Kudr and Jaromir Gumulec and Katerina Tmejova and Marie Konecna and Marketa Vaculovicova and David Hynek and Michal Masarik and Jindrich Kynicky and Rene Kizek and Vojtech Adam},

doi = {10.3390/ijerph110303233},

issn = {1660-4601},

year  = {2014},

date = {2014-03-01},

journal = {Int J Environ Res Public Health},

volume = {11},

number = {3},

pages = {3233--3255},

abstract = {There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24361244,

title = {DNA interaction with zinc(II) ions},

author = {Lukas Nejdl and Branislav Ruttkay-Nedecky and Jiri Kudr and Sona Krizkova and Kristyna Smerkova and Simona Dostalova and Marketa Vaculovicova and Pavel Kopel and Josef Zehnalek and Libuse Trnkova and Petr Babula and Vojtech Adam and Rene Kizek},

doi = {10.1016/j.ijbiomac.2013.12.013},

issn = {1879-0003},

year  = {2014},

date = {2014-03-01},

journal = {Int J Biol Macromol},

volume = {64},

pages = {281--287},

abstract = {We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored using UV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragment of DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 °C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted into a metal bound DNA (Zn-DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum (190-350 nm) and decrease in the melting temperature (Tm) of Zn-DNA. Spectrophotometric (UV/vis) analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at 200 nm and decrease in absorbance at 251 nm. Application of zinc(II) ions at 5.5 μM concentration caused decrease in Tm for app. 7.5 °C in average in comparison with control (75.5 ± 3 °C). The lowest melting temperature (60.5 ± 2.5 °C) was observed after application of zinc(II) ions at 33 μM concentration. Gel electrophoresis proved significance of Zn(II) in the renaturation of DNA. Samples of Zn-DNA (15 μM DNA+5.5-55 μM Zn(II)) caused significant changes in the renaturation of DNA in comparison with the control, untreated DNA (15 μM DNA).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24313683,

title = {Comparison of the effects of silver phosphate and selenium nanoparticles on Staphylococcus aureus growth reveals potential for selenium particles to prevent infection},

author = {Dagmar Chudobova and Kristyna Cihalova and Simona Dostalova and Branislav Ruttkay-Nedecky and Miguel Angel Merlos Rodrigo and Katerina Tmejova and Pavel Kopel and Lukas Nejdl and Jiri Kudr and Jaromir Gumulec and Sona Krizkova and Jindrich Kynicky and Rene Kizek and Vojtech Adam},

doi = {10.1111/1574-6968.12353},

issn = {1574-6968},

year  = {2014},

date = {2014-02-01},

journal = {FEMS Microbiol Lett},

volume = {351},

number = {2},

pages = {195--201},

abstract = {Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 μM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 μg mg(-1) protein) but was increased by 29% after addition of SeNPs (63 μg mg(-1) protein) compared with the S. aureus control (49 μg mg(-1) protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28788525,

title = {Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure},

author = {Monika Kremplova and Dana Fialova and Lukas Nejdl and David Hynek and Libuse Trnkova and Jaromir Hubalek and Rene Kizek and Vojtech Adam},

doi = {10.3390/ma7031455},

issn = {1996-1944},

year  = {2014},

date = {2014-02-01},

journal = {Materials (Basel)},

volume = {7},

number = {3},

pages = {1455--1472},

abstract = {The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected-peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23868417,

title = {Liposomal nanotransporter for targeted binding based on nucleic acid anchor system},

author = {Lukas Nejdl and Miguel Angel Merlos Rodrigo and Jiri Kudr and Branislav Ruttkay-Nedecky and Marie Konecna and Pavel Kopel and Ondrej Zitka and Jaromir Hubalek and Rene Kizek and Vojtech Adam},

doi = {10.1002/elps.201300197},

issn = {1522-2683},

year  = {2014},

date = {2014-02-01},

journal = {Electrophoresis},

volume = {35},

number = {2-3},

pages = {393--404},

abstract = {Microfluidic techniques have been developed intensively in recent years due to lower reagent consumption, faster analysis, and possibility of the integration of several analytical detectors into one chip. Electrochemical detectors are preferred in microfluidic systems, whereas liposomes can be used for amplification of the electrochemical signals. The aim of this study was to design a nanodevice for targeted anchoring of liposome as transport device. In this study, liposome with encapsulated Zn(II) was prepared. Further, gold nanoparticles were anchored onto the liposome surface allowing binding of thiol moiety-modified molecules (DNA). For targeted capturing of the transport device, DNA loops were used. DNA loops were represented by paramagnetic microparticles with oligo(DT)25 chain, on which a connecting DNA was bound. Capturing of transport device was subsequently done by hybridization to the loop. The individual steps were analyzed by electrochemistry and UV/Vis spectrometry. For detection of Zn(II) encapsulated in liposome, a microfluidic system was used. The study succeeded in demonstrating that liposome is suitable for the transport of Zn(II) and nucleic acids. Such transporter may be used for targeted binding using DNA anchor system. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24352014,

title = {Beads-based electrochemical assay for the detection of influenza hemagglutinin labeled with CdTe quantum dots},

author = {Ludmila Krejcova and Lukas Nejdl and David Hynek and Sona Krizkova and Pavel Kopel and Vojtech Adam and Rene Kizek},

doi = {10.3390/molecules181215573},

issn = {1420-3049},

year  = {2013},

date = {2013-12-01},

journal = {Molecules},

volume = {18},

number = {12},

pages = {15573--15586},

abstract = {In this study we describe a beads-based assay for rapid, sensitive and specific isolation and detection of influenza vaccine hemagglutinin (HA). Amplification of the hemagglutinin signal resulted from binding of an electrochemical label as quantum dots (QDs). For detection of the metal and protein part of the resulting HA-CdTe complex, two differential pulse voltammetric methods were used. The procedure includes automated robotic isolation and electrochemical analysis of the isolated product. The isolation procedure was based on the binding of paramagnetic particles (MPs) with glycan (Gly), where glycan was used as the specific receptor for linkage of the QD-labeled hemagglutinin.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24045364,

title = {Relevance of infection with human papillomavirus: the role of the p53 tumor suppressor protein and E6/E7 zinc finger proteins (Review)},

author = {Branislav Ruttkay-Nedecky and Ana Maria Jimenez Jimenez and Lukas Nejdl and Dagmar Chudobova and Jaromir Gumulec and Michal Masarik and Vojtech Adam and Rene Kizek},

doi = {10.3892/ijo.2013.2105},

issn = {1791-2423},

year  = {2013},

date = {2013-12-01},

journal = {Int J Oncol},

volume = {43},

number = {6},

pages = {1754--1762},

abstract = {Human papillomaviruses (HPV) are small circular, double-stranded DNA viruses infecting epithelial tissues. HPV types can be classified both as high-risk or low-risk. Of the more than 120 different identified types of HPV, the majority are involved in infections of the genital tract, cancer of the cervix, vulva, vagina and penis, and of non-anogenital localizations, such as the head and neck areas. From the point of view of the infection, human papillomaviruses have developed several molecular mechanisms to enable infected cells to suppress apoptosis. This review provides a comprehensive and critical summary of the current literature that focuses on cervical carcinoma and cancer of the head and neck caused by HPV. In particular, we discuss HPV virology, the molecular mechanisms of carcinogenesis, the role of the tumor suppressor protein p53 and the E6/E7 zinc finger proteins. Classification of HPV according to diagnosis is also described.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24317385,

title = {Lead ions encapsulated in liposomes and their effect on Staphylococcus aureus},

author = {Renata Kensova and Iva Blazkova and Marie Konecna and Pavel Kopel and Dagmar Chudobova and Ondrej Zitka and Marketa Vaculovicova and David Hynek and Vojtech Adam and Miroslava Beklova and Rene Kizek},

doi = {10.3390/ijerph10126687},

issn = {1660-4601},

year  = {2013},

date = {2013-12-01},

journal = {Int J Environ Res Public Health},

volume = {10},

number = {12},

pages = {6687--6700},

abstract = {The aim of the study was the preparation of a liposome complex with encapsulated lead ions, which were electrochemically detected. In particular, experiments were focused on the potential of using an electrochemical method for the determination of free and liposome-encapsulated lead and determination of the encapsulation efficiency preventing the lead toxicity. Primarily, encapsulation of lead ions in liposomes and confirmation of successful encapsulation by electrochemical methods was done. Further, the reduction effect of the liposome matrix on the detected electrochemical signal was monitored. Besides encapsulation itself, comparison of toxicity of free lead ions and lead ions encapsulated in liposome was tested. The calculated IC50 values for evaluating the lead cytotoxicity showed significant differences between the lead enclosed in liposomes (28 µM) and free lead ions (237 µM). From the cytotoxicity studies on the bacterial strain of S. aureus it was observed that the free lead ions are less toxic in comparison with lead encapsulated in liposomes. Liposomes appear to be a suitable carrier of various substances through the inner cavity. Due to the liposome structure the lead enclosed in the liposome is more easily accepted into the cell structure and the toxicity of the enclosed lead is higher in comparison to free lead ions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23994762,

title = {Investigation of interaction between magnetic silica particles and lambda phage DNA fragment},

author = {Kristyna Smerkova and Simona Dostalova and Marketa Vaculovicova and Jindrich Kynicky and Libuse Trnkova and Miroslav Kralik and Vojtech Adam and Jaromir Hubalek and Ivo Provaznik and Rene Kizek},

doi = {10.1016/j.jpba.2013.07.039},

issn = {1873-264X},

year  = {2013},

date = {2013-12-01},

journal = {J Pharm Biomed Anal},

volume = {86},

pages = {65--72},

abstract = {Nucleic acids belong to the most important molecules and therefore the understanding of their properties, function and behavior is crucial. Even though a range of analytical and biochemical methods have been developed for this purpose, one common step is essential for all of them - isolation of the nucleic acid from the from complex sample matrix. The use of magnetic particles for the separation of nucleic acids has many advantages over other isolation methods. In this study, an isolation procedure for extraction of DNA was optimized. Each step of the isolation process including washing, immobilization and elution was optimized and therefore the efficiency was increased from 1.7% to 28.7% and the total time was shortened from 75 to 30min comparing to the previously described method. Quantification of the particular parameter influence was performed by square-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimized method with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureus and Escherichia coli.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24288003,

title = {G-quadruplexes as sensing probes},

author = {Branislav Ruttkay-Nedecky and Jiri Kudr and Lukas Nejdl and Darina Maskova and Rene Kizek and Vojtech Adam},

doi = {10.3390/molecules181214760},

issn = {1420-3049},

year  = {2013},

date = {2013-11-01},

journal = {Molecules},

volume = {18},

number = {12},

pages = {14760--14779},

abstract = {Guanine-rich sequences of DNA are able to create tetrastranded structures known as G-quadruplexes; they are formed by the stacking of planar G-quartets composed of four guanines paired by Hoogsteen hydrogen bonding. G-quadruplexes act as ligands for metal ions and aptamers for various molecules. Interestingly, the G-quadruplexes form a complex with anionic porphyrin hemin and exhibit peroxidase-like activity. This review focuses on overview of sensing techniques based on G-quadruplex complexes with anionic porphyrins for detection of various analytes, including metal ions such as K+, Ca2+, Ag+, Hg2+, Cu2+, Pb2+, Sr2+, organic molecules, nucleic acids, and proteins. Principles of G-quadruplex-based detection methods involve DNA conformational change caused by the presence of analyte which leads to a decrease or an increase in peroxidase activity, fluorescence, or electrochemical signal of the used probe. The advantages of various detection techniques are also discussed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24233071,

title = {Behaviour of zinc complexes and zinc sulphide nanoparticles revealed by using screen printed electrodes and spectrometry},

author = {Lukas Nejdl and Branislav Ruttkay-Nedecky and Jiří Kudr and Monika Kremplova and Natalia Cernei and Jan Prasek and Marie Konecna and Jaromir Hubalek and Ondrej Zitka and Jindrich Kynicky and Pavel Kopel and Rene Kizek and Vojtech Adam},

doi = {10.3390/s131114417},

issn = {1424-8220},

year  = {2013},

date = {2013-10-01},

journal = {Sensors (Basel)},

volume = {13},

number = {11},

pages = {14417--14437},

abstract = {In this study, we focused on microfluidic electrochemical analysis of zinc complexes (Zn(phen)(his)Cl2, Zn(his)Cl2) and ZnS quantum dots (QDs) using printed electrodes. This method was chosen due to the simple (easy to use) instrumentation and variable setting of flows. Reduction signals of zinc under the strictly defined and controlled conditions (pH, temperature, flow rate, accumulation time and applied potential) were studied. We showed that the increasing concentration of the complexes (Zn(phen)(his)Cl2, Zn(his)Cl2) led to a decrease in the electrochemical signal and a significant shift of the potential to more positive values. The most likely explanation of this result is that zinc is strongly bound in the complex and its distribution on the electrode is very limited. Changing the pH from 3.5 to 5.5 resulted in a significant intensification of the Zn(II) reduction signal. The complexes were also characterized by UV/VIS spectrophotometry, chromatography, and ESI-QTOF mass spectrometry.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23712472,

title = {Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation},

author = {Libor Janu and Maja Stanisavljevic and Sona Krizkova and Pavlina Sobrova and Marketa Vaculovicova and Rene Kizek and Vojtech Adam},

doi = {10.1002/elps.201300088},

issn = {1522-2683},

year  = {2013},

date = {2013-09-01},

journal = {Electrophoresis},

volume = {34},

number = {18},

pages = {2725--2732},

abstract = {The bioconjugation of quantum dots (QDs) is a key process in their application for bioanalysis as well as imaging. The coupling of QDs with biologically active molecules such as peptides, nucleic acids, and/or antibodies enables their fluorescent labeling, and therefore, selective and sensitive tracking during the bioanalytical process, however, the efficiency of the labeling and preservation of the biological activity of the bioconjugate have to be considered. In this study, a new approach of the bioconjugation of CdTe-QDs and human immunoglobulin employing a small peptide is described. The heptapeptide (HWRGWVC) was synthesized and characterized by mass spectrometry, liquid chromatography, and capillary electrophoresis. Moreover, the peptide was used as a capping agent for QDs synthesis. The CdTe-QDs were synthesized by microwave synthesis (600 W, 20 min) using 3.2 mM CdCl2 and 0.8 mM Na2TeO3. The bioconjugation of QDs capped by this peptide with immunoglobulin was investigated by capillary electrophoresis and magnetic immunoextraction coupled with electrochemical detection by differential pulse voltammetry. Furthermore, the applicability of prepared bioconjugates for fluorescent immunodetection was verified using immobilized goat antihuman IgG antibody.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23812079,

title = {Complexes of silver(I) ions and silver phosphate nanoparticles with hyaluronic acid and/or chitosan as promising antimicrobial agents for vascular grafts},

author = {Dagmar Chudobova and Lukas Nejdl and Jaromir Gumulec and Olga Krystofova and Miguel Angel Merlos Rodrigo and Jindrich Kynicky and Branislav Ruttkay-Nedecky and Pavel Kopel and Petr Babula and Vojtech Adam and Rene Kizek},

doi = {10.3390/ijms140713592},

issn = {1422-0067},

year  = {2013},

date = {2013-06-01},

journal = {Int J Mol Sci},

volume = {14},

number = {7},

pages = {13592--13614},

abstract = {Polymers are currently widely used to replace a variety of natural materials with respect to their favourable physical and chemical properties, and due to their economic advantage. One of the most important branches of application of polymers is the production of different products for medical use. In this case, it is necessary to face a significant disadvantage of polymer products due to possible and very common colonization of the surface by various microorganisms that can pose a potential danger to the patient. One of the possible solutions is to prepare polymer with antibacterial/antimicrobial properties that is resistant to bacterial colonization. The aim of this study was to contribute to the development of antimicrobial polymeric material ideal for covering vascular implants with subsequent use in transplant surgery. Therefore, the complexes of polymeric substances (hyaluronic acid and chitosan) with silver nitrate or silver phosphate nanoparticles were created, and their effects on gram-positive bacterial culture of Staphylococcus aureus were monitored. Stages of formation of complexes of silver nitrate and silver phosphate nanoparticles with polymeric compounds were characterized using electrochemical and spectrophotometric methods. Furthermore, the antimicrobial activity of complexes was determined using the methods of determination of growth curves and zones of inhibition. The results of this study revealed that the complex of chitosan, with silver phosphate nanoparticles, was the most suitable in order to have an antibacterial effect on bacterial culture of Staphylococcus aureus. Formation of this complex was under way at low concentrations of chitosan. The results of electrochemical determination corresponded with the results of spectrophotometric methods and verified good interaction and formation of the complex. The complex has an outstanding antibacterial effect and this effect was of several orders higher compared to other investigated complexes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23807501,

title = {Apoferritin modified magnetic particles as doxorubicin carriers for anticancer drug delivery},

author = {Iva Blazkova and Hoai Viet Nguyen and Simona Dostalova and Pavel Kopel and Maja Stanisavljevic and Marketa Vaculovicova and Marie Stiborova and Tomas Eckschlager and Rene Kizek and Vojtech Adam},

doi = {10.3390/ijms140713391},

issn = {1422-0067},

year  = {2013},

date = {2013-06-01},

journal = {Int J Mol Sci},

volume = {14},

number = {7},

pages = {13391--13402},

abstract = {Magnetic particle mediated transport in combination with nanomaterial based drug carrier has a great potential for targeted cancer therapy. In this study, doxorubicin encapsulation into the apoferritin and its conjugation with magnetic particles was investigated by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The quantification of encapsulated doxorubicin was performed by fluorescence spectroscopy and compared to CE-LIF. Moreover, the significant enhancement of the doxorubicin signal was observed by addition of methanol into the sample solution.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23483558,

title = {Integrated chip electrophoresis and magnetic particle isolation used for detection of hepatitis B virus oligonucleotides},

author = {Marketa Vaculovicova and Kristyna Smerkova and Jiri Sedlacek and Jiri Vyslouzil and Jaromir Hubalek and Rene Kizek and Vojtech Adam},

doi = {10.1002/elps.201200697},

issn = {1522-2683},

year  = {2013},

date = {2013-06-01},

journal = {Electrophoresis},

volume = {34},

number = {11},

pages = {1548--1554},

abstract = {Rapid and sensitive detection is a key step in the effective and early response to the global hazard of various viral diseases. In this study, an integrated isolation of hepatitis B virus (HBV)-specific DNA fragment by magnetic nanoparticles (MNPs) and its immediate analysis by microchip CGE was performed. Microfluidic CE chip was used to accommodate the complete process of viral DNA isolation by MNPs including hybridization and thermal denaturation followed by CE separation. Beforehand, calibration curves of HBV fragments were constructed. For isolation by MNPs, specific streptavidin-biotin interaction was used to bind complementary HBV fragment to magnetic particles. After analysis of isolated HBV by regular MNPs method, innovative approach was performed. The commercial CE chip (Bio-rad) was successfully used to execute HBV fragment isolation. Detection using LIF with detection limit of 1 ng/mL was accomplished.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid23502468,

title = {The role of metallothionein in oxidative stress},

author = {Branislav Ruttkay-Nedecky and Lukas Nejdl and Jaromir Gumulec and Ondrej Zitka and Michal Masarik and Tomas Eckschlager and Marie Stiborova and Vojtech Adam and Rene Kizek},

doi = {10.3390/ijms14036044},

issn = {1422-0067},

year  = {2013},

date = {2013-03-01},

journal = {Int J Mol Sci},

volume = {14},

number = {3},

pages = {6044--6066},

abstract = {Free radicals are chemical particles containing one or more unpaired electrons, which may be part of the molecule. They cause the molecule to become highly reactive. The free radicals are also known to play a dual role in biological systems, as they can be either beneficial or harmful for living systems. It is clear that there are numerous mechanisms participating on the protection of a cell against free radicals. In this review, our attention is paid to metallothioneins (MTs) as small, cysteine-rich and heavy metal-binding proteins, which participate in an array of protective stress responses. The mechanism of the reaction of metallothioneins with oxidants and electrophilic compounds is discussed. Numerous reports indicate that MT protects cells from exposure to oxidants and electrophiles, which react readily with sulfhydryl groups. Moreover, MT plays a key role in regulation of zinc levels and distribution in the intracellular space. The connections between zinc, MT and cancer are highlighted.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid24055838,

title = {Quantum dots and prion proteins: is this a new challenge for neurodegenerative diseases imaging?},

author = {Pavlina Sobrova and Iva Blazkova and Jana Chomoucka and Jana Drbohlavova and Marketa Vaculovicova and Pavel Kopel and Jaromir Hubalek and Rene Kizek and Vojtech Adam},

doi = {10.4161/pri.26524},

issn = {1933-690X},

year  = {2013},

date = {2013-01-01},

journal = {Prion},

volume = {7},

number = {5},

pages = {349--358},

abstract = {A diagnostics of infectious diseases can be done by the immunologic methods or by the amplification of nucleic acid specific to contagious agent using polymerase chain reaction. However, in transmissible spongiform encephalopathies, the infectious agent, prion protein (PrP(Sc)), has the same sequence of nucleic acids as a naturally occurring protein. The other issue with the diagnosing based on the PrP(Sc) detection is that the pathological form of prion protein is abundant only at late stages of the disease in a brain. Therefore, the diagnostics of prion protein caused diseases represent a sort of challenges as that hosts can incubate infectious prion proteins for many months or even years. Therefore, new in vivo assays for detection of prion proteins and for diagnosis of their relation to neurodegenerative diseases are summarized. Their applicability and future prospects in this field are discussed with particular aim at using quantum dots as fluorescent labels.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Remote-controlled robotic platform for electrochemical determination of water contaminated by heavy metal ions},

author = {Nejdl, Lukas and Kudr, Jiri and Ruttkay-Nedecky, Branislav and Heger, Zbynek and Zima, L. and Zalud, Ludek and Krizkova, Sona and Adam, Vojtech and Vaculovicova, M. and Kizek, Rene},

url = {http://www.electrochemsci.org/papers/vol10/100403635.pdf},

journal = {International journal of electrochemical science},

volume = {10},

issue = {4},

pages = {3635-3643},

abstract = {The aim of our study was to equip a remote-controlled robotic platform (ORPHEUS-HOPE) with electrochemical system for fast detection of heavy metal ions in water. The platform contains two cameras-one pan/tilt color camera with both manual and automatic parameter settings, and one rigid high resolution color camera for precise vision and zooms to screen printed electrodes at the end of the manipulator. The maximum payload at the end of the manipulator is 0.85 kg. Screen printed electrodes consist with working electrode (WE), reference electrode (RE) and auxiliary electrode (AE).WE electrode (carbon paste) was designed to be as large as possible (in this case geometrically comparable with the diameter of the 3 mm glassy carbon electrode with working area of 7.1 mm2), RE (Ag/AgCl paste) 1.3 mm2 and AE (platinum paste) 6.2 mm2. The analytical parameters of differential pulse voltammetry for remote heavy metals detection (conditional time, step potential, deposition time and temperature) were optimized in this work. Application of robotic platform ORPHEUS-HOPE allowed us to analyze water samples spiked with heavy metal ions in linear ranges as follows:0.8-50 μg.mL-1 in case of Pb(II), 0.04-13 μg.mL-1 in case of Cu(II), 0.2-50 μg.mL-1 Zn(II) and 0.02-25 μg.mL-1 Cd(II). ORPHEUS-HOPE might be helpful for in situ analyzes in environment, possessing conditions incompatible with human presence, such as these after environmental catastrophes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid25476270,

title = {Application of CdTe/ZnSe quantum dots in in vitro imaging of chicken tissue and embryo},

author = {Amitava Moulick and Iva Blazkova and Vedran Milosavljevic and Zdenka Fohlerova and Jaromir Hubalek and Pavel Kopel and Marketa Vaculovicova and Vojtech Adam and Rene Kizek},

doi = {10.1111/php.12398},

issn = {1751-1097},

journal = {Photochem Photobiol},

volume = {91},

number = {2},

pages = {417--423},

abstract = {The present work is aimed to synthesize CdTe/ZnSe core/shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in in vitro imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time-consuming method. Subsequently, these QDs were characterized by spectrofluorimetry, Transmission Electron Microscopy, X-ray fluorescence analysis and Dynamic Light Scattering measurement. A blueshifting of the emission was found when ZnSe was deposited on CdTe QDs. The QDs showed its fluorescence emission quantum yields up to 25%. They were applied into chicken embryos and breast muscle tissues to study their efficiency in in vitro imaging. All the QDs of different color were able to visualize in in vitro imaging. The highest fluorescence intensity was detected in the case of red QDs prepared at 100°C. The green and red QDs were possible to detect up to the depth of 3 and 4 mm of the tissue, respectively. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}