Laboratory of bioanalysis and imaging

Laboratory of Bioanalysis and Imaging focuses on analysis of a wide range of biological, clinical and environmental samples. The laboratory combines classical analytical approaches with use of electromigration methods (classical instrumental design, chip-based, modular portable systems, etc.) with absorption, conductivity, or laser/LED-induced fluorescence detection.

The laboratory also develops and tests micro-flow separation and detection systems based on the LabSmith kit or lateral micro-flow systems on paper carriers for point of care applications. The laboratory also focuses on the synthesis of a wide range of nanoparticles and nanostructured materials (in particular, semiconductor nanocrystals (quantum dots), upconversion nanoparticles and metal-based nanoparticles (zinc, copper and selenium)). These advanced materials are further used as a basis for bio-sensors to enhance the separation efficiency of capillary electrophoresis, biomolecule labeling and in vivo / in vitro imaging. The aim of in vivo imaging is to use advanced “smart” materials for a) real-time diagnostics of physiological and pathological processes in model organisms (small mammals), b) drug transport and c) theranostic applications, i.e. applications combining drug transport, its release i the target site (therapy), and subsequent imaging (diagnosis).

Ing. Lukáš Nejdl, Ph.D.

Head – Laboratory of bioanalysis and imaging
Assistant Professor

Phone: 420545 13 32 90
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3010
Email: lukasnejdl@gmail.com

Team members

  • Ing. Lukáš Nejdl, Ph.D.
  • prof. RNDr. Miroslav Macka, Ph.D.
  • Ing. Jaroslava Bezděková, Ph.D.
  • Ing. Kristýna Pavelicová
  • Ing. Kristýna Zemánková
  • Mgr. Tomáš Rýpar
  • Mgr. Marcela Vlčnovská
  • Ing. Bc. Milada Vodová

Topics of Ph.D. theses

  • Optional topic
  • Paperfluidic analytical devices for applications in diagnostics
  • Optical detection techniques for bioanalysis

Projects

  • GAČR: Paperfluidická přenosná zařizení pro rychlou a nízkonákladovou analýzu bez instrumentální detekce. 2019-2021.

Publications

  • KHUNKITCHAI, N.,NUCHTAVORN, N.,RYPAR, T.,VLCNOVSKA, M.,NEJDL, L.,VACULOVICOVA, M.,MACKA, M. Uv-light-actuated in-situ preparation of paper@zncd quantum dots for paper-based enzymatic nanoreactors. Chemical Engineering Journal. 2022, vol. 428. no., pp. 132508.
  • VODOVA, M.,NEJDL, L.,PAVELICOVA, K.,ZEMANKOVA, K.,RYPAR, T.,SKOPALOVA STERBOVA, D.,BEZDEKOVA, J.,NUCHTAVORN, N.,MACKA, M.,ADAM, V.,VACULOVICOVA, M. Detection of pesticides in food products using paper-based devices by uv-induced fluorescence spectroscopy combined with molecularly imprinted polymers. Food Chemistry. 2022, vol. 380. no., pp. 132141.
  • ZEMANKOVA, K.,NEJDL, L.,BEZDEKOVA, J.,VODOVA, M.,PETERA, L.,PASTOREK, A.,CIVIS, S.,KUBELIK, P.,FERUS, M.,ADAM, V.,VACULOVICOVA, M. Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry. Microchemical Journal. 2021, vol. 167. no., pp. 7.
  • NEJDL, L.,HAVLIKOVA, M.,MRAVEC, F.,VACULOVIC, T.,FALTUSOVA, V.,PAVELICOVA, K.,BARON, M.,KUMSTA, M.,ONDROUSEK, V.,ADAM, V.,VACULOVICOVA, M. Uv-induced fingerprint spectroscopy. Food Chemistry. 2022, vol. 368. no., pp. 130499.
  • PAVELICOVA, K.,VANICKOVA, L.,HADDAD, Y.,NEJDL, L.,ZITKA, J.,KOCIOVA, S.,MRAVEC, F.,VACULOVIC, T.,MACKA, M.,VACULOVICOVA, M.,ADAM, V. Metallothionein dimerization evidenced by qd-based förster resonance energy transfer and capillary electrophoresis. International Journal of Biological Macromolecules. 2021, vol. 170. no., pp. 53-60.
  • Rypar, T., Adam, V., Vaculovicova, M. & Macka, M. Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers. Sens. Actuator B-Chem. 341, 1-10, doi:https://doi.org/10.1016/j.snb.2021.129999 (2021).
  • Pavelicova, K. et al. Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis. Int. J. Biol. Macromol. 170, 53-60 (2021).
  • Nejdl, L. et al. UV-Induced Fingerprint Spectroscopy. Food Chem. in press (2021).
  • Vaneckova, T., Bezdekova, A., Han, G., Adam, V. & Vaculovicova, M. Application of molecularly imprinted polymers as artificial receptors for imaging. Acta Biomater. 101, 444-458, doi:10.1016/j.actbio.2019.11.007 (2020).
  • Tesarova, B. et al. Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine. Appl. Mater. Today 18, 1-11, doi:https://doi.org/10.1016/j.apmt.2019.100501 (2020).
  • Pastorek, A. et al. Prebiotic synthesis at impact craters: the role of Fe-clays and iron meteorites. Chem. Commun. 55, 10563-10566, doi:10.1039/c9cc04627e (2019).
  • Huang, L. et al. Designing next generation of photon upconversion: Recent advances in organic triplet-triplet annihilation upconversion nanoparticles. Biomaterials 201, 77-86, doi:10.1016/j.biomaterials.2019.02.008 (2019).
  • Stanisavljevic, M., Krizkova, S., Vaculovicova, M., Kizek, R. & Adam, V. Quantum dots-fluorescence resonance energy transfer-based nano-sensors and their application. Biosens. Bioelectron. 74, 562-574, doi:10.1016/j.bios.2015.06.076 (2015).
  • Nuchtavorn, N., Leanpolchareanchai, J., Suntornsuk, L. & Macka, M. Paper-based sol-gel thin films immobilized cytochrome P450 for enzyme activity measurement. Anal. Chim. Acta 1098, 86-93, doi:https://doi.org/10.1016/j.aca.2019.11.031 (2020).
  • Aydofan, C. et al. Miniaturized LC in Molecular Omics. Anal. Chem. 92, 11485-11497, doi:https://doi.org/10.1021/acs.analchem.0c01436 (2020).
  • Rahbar, M., Wheeler, A. R., Paull, B. & Macka, M. Ion-exchange based immobilization of chromogenic reagents on microfluidic paper analytical devices. Anal. Chem. 91, 8756-8761, doi:10.1021/acs.analchem.9b01288 (2019).
  • Alves, M. N., Miro, M., Breadmore, M. C. & Macka, M. Trends in analytical separations of magnetic (nano)particles. TRAC-Trends Anal. Chem. 114, 89-97, doi:10.1016/j.trac.2019.02.026 (2019).