Laboratoř prvkové a speciační analýzy je výzkumně zaměřena především na analýzy stopových prvků, ale i mikro a makro prvků (Cd, Pb, Hg, Ni, Cr, Pt, Zn, Fe, Cu, Mn, Co, Ca, Mg, Te, Au, Ag, Se), jak v environmentálních a biologických vzorcích, tak i v potravinách rostlinného a živočišného původu. Pro stanovení těchto prvků využíváme metodu AAS a jednotlivé specie rtuti (Hg2+, MeHg+, EtHg+, PhHg+) analyzujeme metodou HPLC-CV-AFS.
Jedním z našich hlavních výzkumných záměrů je studium difúzních a kinetických procesů probíhajících v životním prostředí s využitím DGT (techniky difúzního gradientu v tenkém filmu) k analýze biodostupných forem, především těžkých kovů.
Ing. Andrea Ridošková, Ph.D.
Vedoucí laboratoře prvkové a speciační analýzy
Akademická pracovnice – odborná asistentka
Telefon: 420 545 133 276
Adresa pracoviště: ÚCB AF, Zemědělská 1, 61300 Brno – Budova D
Označení kanceláře: BA02N3006
E-mail: Andrea.klr@seznam.cz
Researcher ID: A-6765-2016
ORCID: 0000-0002-4650-6379
Členové týmu
- Ing. Andrea Ridošková, Ph.D.
- doc. Mgr. Pavlína Pelcová, Ph.D.
- Ing. Vendula Smolíková
- Ing. Peter Csányi
- Mgr. Michal Morávek
Vypsaná témata disertačních prací
- Volné téma-kovy v životním prostředí
- Biologická dostupnost rtuti pro užitkové plodiny
Projekty
- Mobility ČR-Francie – Využití fyzikálně-chemických metod pro odhad biodostupných forem arsenu v zemědělských, urbánních a lesních půdách s LGCgE-ISA Lille, Yncréa Hauts-de-France (2020-2021)
- GAČR: Technika difúzního gradientu v tenkém filmu: účinný nástroj pro předpovídání biologické dostupnosti rtuti (2019-2021).
- OP VVV: European Regional Development Fund in the Operational Programme Research, Development and Education: Udržitelná produkce zdravých ryb v různých akvakulturních systémech – PROFISH (2019-2023).
- Týmový projekt IGA AF-IGA2020-TP010 (2020-2021).
Zahraniční spolupráce
- LGCgE (Laboratoire Génie Civil et géo-Environnement), Junia – ISA Lille, France
- Vrije Universiteit Brussel, Belgium
Nejvýznamnější publikace skupiny za posledních 5 let
-
Pelcová, Pavlína — Kopp, Radovan — Ridošková, Andrea — Grmela, Jan — Štěrbová, DagmarEvaluation of mercury bioavailability and phytoaccumulation by means of a DGT technique and of submerged aquatic plants in an aquatic ecosystem situated in the vicinity of a cinnabar mine. Chemosphere. 2022. sv. 288, č. February, ISSN 0045-6535. URL: https://doi.org/10.1016/j.
chemosphere.2021.132545 -
Ridošková, Andrea — Pelfrene, Aurelie — Douay, Francis — Pelcová, Pavlína — Smolíková, Vendula — Adam, Vojtěch
Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus x giganteus. Environmental Toxicology and Chemistry. 2019. sv. 38, č. 2, s. 321–328. ISSN 0730-7268. URL: https://setac.onlinelibrary.wiley.com/doi/full/10.1002/ etc.4318 -
Pelcová, Pavlína — Ridošková, Andrea — Hrachovinová, Jana — Grmela, Jan
Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine. Environmental Pollution. 2021. sv. 283, č. 15 August, ISSN 0269-7491. URL: https://doi.org/10.1016/j.envpol.2021.117092 -
Smolíková, Vendula — Pelcová, Pavlína — Ridošková, Andrea — Leermakers, Martine
Simultaneous determination of arsenic and uranium by the diffusive gradients in thin films technique using Lewatit FO 36: Optimization of elution protocol. Talanta. 2021. sv. 228, č. 1 June, ISSN 0039-9140. URL: https://doi.org/10.1016/j.talanta.2021.122234
2022
Pelcová, Pavlína; Kopp, Radovan; Ridošková, Andrea; Grmela, Jan; Štěrbová, Dagmar
In: Chemosphere, vol. 288, no. Pt 2, pp. 132545, 2022, ISSN: 1879-1298.
@article{pmid34648791,
title = {Evaluation of mercury bioavailability and phytoaccumulation by means of a DGT technique and of submerged aquatic plants in an aquatic ecosystem situated in the vicinity of a cinnabar mine},
author = {Pavlína Pelcová and Radovan Kopp and Andrea Ridošková and Jan Grmela and Dagmar Štěrbová},
doi = {10.1016/j.chemosphere.2021.132545},
issn = {1879-1298},
year = {2022},
date = {2022-02-01},
journal = {Chemosphere},
volume = {288},
number = {Pt 2},
pages = {132545},
abstract = {The ability of submerged aquatic plants (Elodea canadensis, Myriophyllum spicatum, Ceratophyllum demersum) and a natant plant (Eichhornia crassipes) to bioaccumulate mercury was evaluated in a laboratory experiment as well as in a real aquatic ecosystem situated in the vicinity of a cinnabar mine. Moreover, the ability of the diffusive gradients in the thin films technique (DGT) to predict mercury bioavailability for selected aquatic plants was tested. The submerged plants had sufficient bioaccumulation capacity for long-term phytoaccumulation of mercury in a real aquatic ecosystem. The determined bioaccumulation factor was greater than 1000. On average, the submerged plant leaves accumulated 13 times more mercury than the leaves of the natant aquatic plants. Chlorides at concentrations up to 200 mg/L had no statistically significant effect on mercury accumulation, nevertheless, the presence of humic acid in the water environment resulted in its significant (p < 0.002) decrease. A strong positive correlation (r > 0.66) was determined between mercury concentration in the input parts (leaves and/or roots) of the aquatic plants and the flow of mercury into DGT units.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The ability of submerged aquatic plants (Elodea canadensis, Myriophyllum spicatum, Ceratophyllum demersum) and a natant plant (Eichhornia crassipes) to bioaccumulate mercury was evaluated in a laboratory experiment as well as in a real aquatic ecosystem situated in the vicinity of a cinnabar mine. Moreover, the ability of the diffusive gradients in the thin films technique (DGT) to predict mercury bioavailability for selected aquatic plants was tested. The submerged plants had sufficient bioaccumulation capacity for long-term phytoaccumulation of mercury in a real aquatic ecosystem. The determined bioaccumulation factor was greater than 1000. On average, the submerged plant leaves accumulated 13 times more mercury than the leaves of the natant aquatic plants. Chlorides at concentrations up to 200 mg/L had no statistically significant effect on mercury accumulation, nevertheless, the presence of humic acid in the water environment resulted in its significant (p < 0.002) decrease. A strong positive correlation (r > 0.66) was determined between mercury concentration in the input parts (leaves and/or roots) of the aquatic plants and the flow of mercury into DGT units.
2021
Janova, Anna; Kolackova, Martina; Bytesnikova, Zuzana; Capal, Petr; Chaloupsky, Pavel; Svec, Pavel; Ridoskova, Andrea; Cernei, Natalia; Klejdus, Borivoj; Richtera, Lukas; Adam, Vojtech; Huska, Dalibor
In: Algal Research, vol. 60, 2021.
@article{nokey,
title = {New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites},
author = {Anna Janova and Martina Kolackova and Zuzana Bytesnikova and Petr Capal and Pavel Chaloupsky and Pavel Svec and Andrea Ridoskova and Natalia Cernei and Borivoj Klejdus and Lukas Richtera and Vojtech Adam and Dalibor Huska},
doi = {https://doi.org/10.1016/j.algal.2021.102476},
year = {2021},
date = {2021-09-03},
urldate = {2021-09-03},
journal = {Algal Research},
volume = {60},
abstract = {The effects of copper nanoparticles (Cu-NPs), including their stability in the medium, were studied with the green unicellular algae Chlamydomonas reinhardtii (CC-125). Cu-NPs were synthesized and characterized. Cu-NP particles were uniform, regular, and largely spherical, and they had smooth surfaces; the average size was estimated to be 137.4 ± 2.1 nm. Chlamydomonas cells were cultivated for 96 h under controlled conditions in the presence of Cu-NPs, according to OECD guidelines, and then subjected to toxicological bioassays. Based on scanning electron microscopy (SEM) observations, the effects of Cu-NPs resulted in part from the dissolution of nanoparticles (NPs) and the action of copper itself, which shows the importance of studying NP stability in the testing environment. In this assay, deleterious effects were enhanced by increasing Cu-NP concentrations (5, 10, 25, 50, and 100 mg/L). Concentrations higher than 25 mg/L exhibited extreme toxicity. We confirmed the known toxic effects of metal NPs, namely, growth inhibition, reduction of chlorophyll levels in cells, cell penetration and increased ROS production. Attention was also paid to select underexplored metabolites, which were studied with a LC-MS/MS system. Treatments caused changes in metabolites profiles, and levels of p-hydroxybenzaldehyde and protocatechuic acid were especially enhanced, suggesting their positive roles in the antioxidant defence response. Furthermore, a repeatable increase in suberic acid levels was observed for various stress conditions tested, and we expect that this was the result of lipid peroxidation.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The effects of copper nanoparticles (Cu-NPs), including their stability in the medium, were studied with the green unicellular algae Chlamydomonas reinhardtii (CC-125). Cu-NPs were synthesized and characterized. Cu-NP particles were uniform, regular, and largely spherical, and they had smooth surfaces; the average size was estimated to be 137.4 ± 2.1 nm. Chlamydomonas cells were cultivated for 96 h under controlled conditions in the presence of Cu-NPs, according to OECD guidelines, and then subjected to toxicological bioassays. Based on scanning electron microscopy (SEM) observations, the effects of Cu-NPs resulted in part from the dissolution of nanoparticles (NPs) and the action of copper itself, which shows the importance of studying NP stability in the testing environment. In this assay, deleterious effects were enhanced by increasing Cu-NP concentrations (5, 10, 25, 50, and 100 mg/L). Concentrations higher than 25 mg/L exhibited extreme toxicity. We confirmed the known toxic effects of metal NPs, namely, growth inhibition, reduction of chlorophyll levels in cells, cell penetration and increased ROS production. Attention was also paid to select underexplored metabolites, which were studied with a LC-MS/MS system. Treatments caused changes in metabolites profiles, and levels of p-hydroxybenzaldehyde and protocatechuic acid were especially enhanced, suggesting their positive roles in the antioxidant defence response. Furthermore, a repeatable increase in suberic acid levels was observed for various stress conditions tested, and we expect that this was the result of lipid peroxidation.
Pelcová, Pavlína; Ridošková, Andrea; Hrachovinová, Jana; Grmela, Jan
Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine Journal Article
In: Environ Pollut, vol. 283, pp. 117092, 2021, ISSN: 1873-6424.
@article{pmid33892369,
title = {Evaluation of mercury bioavailability to vegetables in the vicinity of cinnabar mine},
author = {Pavlína Pelcová and Andrea Ridošková and Jana Hrachovinová and Jan Grmela},
doi = {10.1016/j.envpol.2021.117092},
issn = {1873-6424},
year = {2021},
date = {2021-08-01},
journal = {Environ Pollut},
volume = {283},
pages = {117092},
abstract = {Knowledge of the concentration of the bioavailable forms of mercury in the soil is necessary, especially, if these soils contain above-limit total mercury concentrations. The bioavailability of mercury in soil samples collected from the vicinity of abandoned cinnabar mines was evaluated using diffusive gradients in the thin films technique (DGT) and mercury phytoaccumulation by vegetables (lettuce, spinach, radish, beetroot, carrot, and green peas). Mercury was accumulated primarily in roots of vegetables. The phytoaccumulation of mercury into edible plant parts was site-specific as well as vegetable species-specific. The mercury concentration in edible parts decreased in the order: spinach leaf ≥ lettuce leaf ≥ carrot storage root ≥ beetroot storage root > radish storage root > pea legume. The translocation index as well as the target hazard quotient indicate the possible usability of soils from the vicinity of abandoned cinnabar mines for planting pod vegetables (peas). A strong positive correlation (r = 0.75 to 0.92, n > 30, p < 0.05) was observed between mercury concentration in secondary roots, the storage roots, leaves of vegetables and the flux of mercury from soil to the DGT units, and the effective concentration of mercury in soil solutions.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Knowledge of the concentration of the bioavailable forms of mercury in the soil is necessary, especially, if these soils contain above-limit total mercury concentrations. The bioavailability of mercury in soil samples collected from the vicinity of abandoned cinnabar mines was evaluated using diffusive gradients in the thin films technique (DGT) and mercury phytoaccumulation by vegetables (lettuce, spinach, radish, beetroot, carrot, and green peas). Mercury was accumulated primarily in roots of vegetables. The phytoaccumulation of mercury into edible plant parts was site-specific as well as vegetable species-specific. The mercury concentration in edible parts decreased in the order: spinach leaf ≥ lettuce leaf ≥ carrot storage root ≥ beetroot storage root > radish storage root > pea legume. The translocation index as well as the target hazard quotient indicate the possible usability of soils from the vicinity of abandoned cinnabar mines for planting pod vegetables (peas). A strong positive correlation (r = 0.75 to 0.92, n > 30, p < 0.05) was observed between mercury concentration in secondary roots, the storage roots, leaves of vegetables and the flux of mercury from soil to the DGT units, and the effective concentration of mercury in soil solutions.
Smolíková, Vendula; Pelcová, Pavlína; Ridošková, Andrea; Leermakers, Martine
In: Talanta, vol. 228, pp. 122234, 2021, ISSN: 1873-3573.
@article{pmid33773713,
title = {Simultaneous determination of arsenic and uranium by the diffusive gradients in thin films technique using Lewatit FO 36: Optimization of elution protocol},
author = {Vendula Smolíková and Pavlína Pelcová and Andrea Ridošková and Martine Leermakers},
doi = {10.1016/j.talanta.2021.122234},
issn = {1873-3573},
year = {2021},
date = {2021-06-01},
journal = {Talanta},
volume = {228},
pages = {122234},
abstract = {The sorption ability of Lewatit FO 36-DGT resin gel, which has been developed for arsenic determination, towards uranium was tested by batch experiments within this study for the first time. Since the uptake efficiency of uranium was 99.0 ± 0.4% and the maximum uptake capacity was not achieved even at the U spike of 1250 μg in the solution, the Lewatit FO 36 resin seems to be a suitable binding phase for DGT resin gels for the determination of uranium. The resin gel also does not display any significant sorption selectivity in favour of one element over another. A novel protocol for simultaneous elution of arsenic and uranium from Lewatit FO 36 resin gel was therefore proposed in this study. The elution efficiencies of 90.3 ± 3.9% and 85.2 ± 3.1% for As and U, respectively, were obtained using 5 mL of 1 M NaOH at 70 °C for 24 h. The comparison with the original elution protocol using microwave-assisted elution by 0.25 M NaOH and 0.17 M NaCl at 130 °C for 16 min indicates, that the novel elution protocol provides good results in the performance of arsenic elution and, in addition, allows simultaneous elution of uranium. Moreover, the elimination of NaCl from the elution process allows a fast and simple analysis of both elements using ICP-MS, and therefore, the Lewatit FO 36-DGT technique can become more commonplace among laboratories without the need to modify the analytical method as proposed in the original study.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The sorption ability of Lewatit FO 36-DGT resin gel, which has been developed for arsenic determination, towards uranium was tested by batch experiments within this study for the first time. Since the uptake efficiency of uranium was 99.0 ± 0.4% and the maximum uptake capacity was not achieved even at the U spike of 1250 μg in the solution, the Lewatit FO 36 resin seems to be a suitable binding phase for DGT resin gels for the determination of uranium. The resin gel also does not display any significant sorption selectivity in favour of one element over another. A novel protocol for simultaneous elution of arsenic and uranium from Lewatit FO 36 resin gel was therefore proposed in this study. The elution efficiencies of 90.3 ± 3.9% and 85.2 ± 3.1% for As and U, respectively, were obtained using 5 mL of 1 M NaOH at 70 °C for 24 h. The comparison with the original elution protocol using microwave-assisted elution by 0.25 M NaOH and 0.17 M NaCl at 130 °C for 16 min indicates, that the novel elution protocol provides good results in the performance of arsenic elution and, in addition, allows simultaneous elution of uranium. Moreover, the elimination of NaCl from the elution process allows a fast and simple analysis of both elements using ICP-MS, and therefore, the Lewatit FO 36-DGT technique can become more commonplace among laboratories without the need to modify the analytical method as proposed in the original study.
Nsanganwimana, Florien; Souki, Karim Suhail Al; Waterlot, Christophe; Douay, Francis; Pelfrêne, Aurélie; Ridošková, Andrea; Louvel, Brice; Pourrut, Bertrand
Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment Journal Article
In: Ecotoxicol Environ Saf, vol. 214, pp. 112125, 2021, ISSN: 1090-2414.
@article{pmid33714138,
title = {Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment},
author = {Florien Nsanganwimana and Karim Suhail Al Souki and Christophe Waterlot and Francis Douay and Aurélie Pelfrêne and Andrea Ridošková and Brice Louvel and Bertrand Pourrut},
doi = {10.1016/j.ecoenv.2021.112125},
issn = {1090-2414},
year = {2021},
date = {2021-05-01},
journal = {Ecotoxicol Environ Saf},
volume = {214},
pages = {112125},
abstract = {Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5-45.3%, whereas CaCl-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01-0.06, 0.11-1.15 and 0.09-0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Phytomanagement is proposed as a cost-effective and environmentally-friendly suggestion for sustainable use of large metal-contaminated areas. In the current work, the energy crop miscanthus (Miscanthus × giganteus) was grown in ex situ conditions on agricultural soils presenting a Cd, Pb and Zn contamination gradient. After 93 days of culture, shoot and root growth parameters were measured. Soils and plants were sampled as well to study the TE accumulation in miscanthus and the effects of this plant on TE mobility in soils. Results demonstrated that miscanthus growth depended more on the soils silt content rather than TE-contamination level. Moreover, soil organic carbon at T93 increased in the soils after miscanthus cultivation by 25.5-45.3%, whereas CaCl-extractible TEs decreased due to complex rhizosphere processes driving plant mineral uptake, and organic carbon inputs into the rhizosphere. In the contaminated soils, miscanthus accumulated Cd, Pb and Zn mainly in roots (BCF in roots: Cd " Zn > Pb), while strongly reducing the transfer of these elements from soil to all organs and from roots to rhizomes, stems and leaves (average TFs: 0.01-0.06, 0.11-1.15 and 0.09-0.79 corresponding to Cd, Pb and Zn respectively). Therefore, miscanthus could be considered a TE-excluder, hence a potential candidate crop for coupling phytostabilization and biomass production on the studied Metaleurop TE-contaminated soils.
Stepankova, Hana; Swiatkowski, Marcin; Kruszynski, Rafal; Svec, Pavel; Michalkova, Hana; Smolikova, Vendula; Ridoskova, Andrea; Splichal, Zbynek; Michalek, Petr; Richtera, Lukas; Kopel, Pavel; Adam, Vojtech; Heger, Zbynek; Rex, Simona
In: Int J Nanomedicine, vol. 16, pp. 4431–4449, 2021, ISSN: 1178-2013.
@article{pmid34234435,
title = {The Anti-Proliferative Activity of Coordination Compound-Based ZnO Nanoparticles as a Promising Agent Against Triple Negative Breast Cancer Cells},
author = {Hana Stepankova and Marcin Swiatkowski and Rafal Kruszynski and Pavel Svec and Hana Michalkova and Vendula Smolikova and Andrea Ridoskova and Zbynek Splichal and Petr Michalek and Lukas Richtera and Pavel Kopel and Vojtech Adam and Zbynek Heger and Simona Rex},
doi = {10.2147/IJN.S304902},
issn = {1178-2013},
year = {2021},
date = {2021-01-01},
journal = {Int J Nanomedicine},
volume = {16},
pages = {4431--4449},
abstract = {PURPOSE: The present study deals with the in vitro evaluation of the potential use of coordination compound-based zinc oxide (ZnO) nanoparticles (NPs) for the treatment of triple negative breast cancer cells (TNBrCa). As BrCa is one of the most prevalent cancer types and TNBrCa treatment is difficult due to poor prognosis and a high metastasis rate, finding a more reliable treatment option should be of the utmost interest.
METHODS: Prepared by reacting zinc carboxylates (formate, acetate, propionate, butyrate, isobutyrate, valerate) and hexamethylenetetramine, 4 distinct coordination compounds were further subjected to two modes of conversion into ZnO NPs - ultrasonication with oleic acid or heating of pure precursors in an air atmosphere. After detailed characterization, the resulting ZnO NPs were subjected to in vitro testing of cytotoxicity toward TNBrCa and normal breast epithelial cells. Further, their biocompatibility was evaluated.
RESULTS: The resulting ZnO NPs provide distinct morphological features, size, biocompatibility, and selective cytotoxicity toward TNBrCa cells. They internalize into two types of TNBrCa cells and imbalance their redox homeostasis, influencing their metabolism, morphology, and ultimately leading to their death via apoptosis or necrosis.
CONCLUSION: The crucial properties of ZnO NPs seem to be their morphology, size, and zinc content. The ZnO NPs with the most preferential values of all three properties show great promise for a future potential use in the therapy of TNBrCa.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
PURPOSE: The present study deals with the in vitro evaluation of the potential use of coordination compound-based zinc oxide (ZnO) nanoparticles (NPs) for the treatment of triple negative breast cancer cells (TNBrCa). As BrCa is one of the most prevalent cancer types and TNBrCa treatment is difficult due to poor prognosis and a high metastasis rate, finding a more reliable treatment option should be of the utmost interest.
METHODS: Prepared by reacting zinc carboxylates (formate, acetate, propionate, butyrate, isobutyrate, valerate) and hexamethylenetetramine, 4 distinct coordination compounds were further subjected to two modes of conversion into ZnO NPs - ultrasonication with oleic acid or heating of pure precursors in an air atmosphere. After detailed characterization, the resulting ZnO NPs were subjected to in vitro testing of cytotoxicity toward TNBrCa and normal breast epithelial cells. Further, their biocompatibility was evaluated.
RESULTS: The resulting ZnO NPs provide distinct morphological features, size, biocompatibility, and selective cytotoxicity toward TNBrCa cells. They internalize into two types of TNBrCa cells and imbalance their redox homeostasis, influencing their metabolism, morphology, and ultimately leading to their death via apoptosis or necrosis.
CONCLUSION: The crucial properties of ZnO NPs seem to be their morphology, size, and zinc content. The ZnO NPs with the most preferential values of all three properties show great promise for a future potential use in the therapy of TNBrCa.
METHODS: Prepared by reacting zinc carboxylates (formate, acetate, propionate, butyrate, isobutyrate, valerate) and hexamethylenetetramine, 4 distinct coordination compounds were further subjected to two modes of conversion into ZnO NPs - ultrasonication with oleic acid or heating of pure precursors in an air atmosphere. After detailed characterization, the resulting ZnO NPs were subjected to in vitro testing of cytotoxicity toward TNBrCa and normal breast epithelial cells. Further, their biocompatibility was evaluated.
RESULTS: The resulting ZnO NPs provide distinct morphological features, size, biocompatibility, and selective cytotoxicity toward TNBrCa cells. They internalize into two types of TNBrCa cells and imbalance their redox homeostasis, influencing their metabolism, morphology, and ultimately leading to their death via apoptosis or necrosis.
CONCLUSION: The crucial properties of ZnO NPs seem to be their morphology, size, and zinc content. The ZnO NPs with the most preferential values of all three properties show great promise for a future potential use in the therapy of TNBrCa.
2020
Smolíková, Vendula; Pelcová, Pavlína; Ridošková, Andrea; Hedbávný, Josef; Grmela, Jan
Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique Journal Article
In: Anal Chim Acta, vol. 1102, pp. 36–45, 2020, ISSN: 1873-4324.
@article{pmid32043994,
title = {Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique},
author = {Vendula Smolíková and Pavlína Pelcová and Andrea Ridošková and Josef Hedbávný and Jan Grmela},
doi = {10.1016/j.aca.2019.12.042},
issn = {1873-4324},
year = {2020},
date = {2020-03-01},
journal = {Anal Chim Acta},
volume = {1102},
pages = {36--45},
abstract = {An ion-exchange resin Lewatit FO 36 was used for the preparation of a new resin gel for the diffusive gradient in thin films technique (DGT). The DGT method was optimized for the accumulation of four bioavailable arsenic species (As, As, monomethylarsonic acid, dimethylarsinic acid) in the aquatic environment. The total sorption capacity of Lewatit FO 36 resin gel was 535 μg As disc. The microwave-assisted extraction in the presence of NaCl (10 g L) and NaOH (10 g L) was used for the isolation of arsenic species from the Lewatit FO 36 resin gel. The elution efficiency of arsenic was 98.4 ± 2.0%. Arsenic was determined by the optimized electrothermal atomic absorption spectrometry (ET-AAS) method using palladium modifier, pre-atomization cool-down step and tungsten carbides coating of graphite tube. The Lewatit FO 36 resin gel provides accurate results (c/c ratio 0.86-1.00) in the pH range 4-8. No significant influence of experimental conditions was observed in the presence of chlorides (0-0.5 mol L) and humic acid (0-100 mg L). Only a very high concentration of phosphates (10 mg L) caused a slight decrease in the diffusion coefficients of MMA and As species (8.4% and 12.4%, respectively). The presence of iron (0-1 mg L) caused a decrease in the diffusion coefficients, but with regard to the common concentrations of iron (less than 0.3 mg L), the negative effect was considered not significant for As and DMA in natural water. The DGT-ET-AAS method was applied for the determination of bioavailable arsenic species in the spiked river water samples and also in-situ in the water reservoir. The new resin gel was characterized by a homogeneous gel structure with excellent reproducibility (< 5% variation of results between batches) and high sorption capacity which suggests its possible long-term application (up to 286 days in the environment with the arsenic concentration of 100 μg L).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
An ion-exchange resin Lewatit FO 36 was used for the preparation of a new resin gel for the diffusive gradient in thin films technique (DGT). The DGT method was optimized for the accumulation of four bioavailable arsenic species (As, As, monomethylarsonic acid, dimethylarsinic acid) in the aquatic environment. The total sorption capacity of Lewatit FO 36 resin gel was 535 μg As disc. The microwave-assisted extraction in the presence of NaCl (10 g L) and NaOH (10 g L) was used for the isolation of arsenic species from the Lewatit FO 36 resin gel. The elution efficiency of arsenic was 98.4 ± 2.0%. Arsenic was determined by the optimized electrothermal atomic absorption spectrometry (ET-AAS) method using palladium modifier, pre-atomization cool-down step and tungsten carbides coating of graphite tube. The Lewatit FO 36 resin gel provides accurate results (c/c ratio 0.86-1.00) in the pH range 4-8. No significant influence of experimental conditions was observed in the presence of chlorides (0-0.5 mol L) and humic acid (0-100 mg L). Only a very high concentration of phosphates (10 mg L) caused a slight decrease in the diffusion coefficients of MMA and As species (8.4% and 12.4%, respectively). The presence of iron (0-1 mg L) caused a decrease in the diffusion coefficients, but with regard to the common concentrations of iron (less than 0.3 mg L), the negative effect was considered not significant for As and DMA in natural water. The DGT-ET-AAS method was applied for the determination of bioavailable arsenic species in the spiked river water samples and also in-situ in the water reservoir. The new resin gel was characterized by a homogeneous gel structure with excellent reproducibility (< 5% variation of results between batches) and high sorption capacity which suggests its possible long-term application (up to 286 days in the environment with the arsenic concentration of 100 μg L).
Horky, Pavel; Venusova, Eva; Aulichova, Tereza; Ridoskova, Andrea; Skladanka, Jiri; Skalickova, Sylvie
Usability of graphene oxide as a mycotoxin binder: In vitro study Journal Article
In: PLoS One, vol. 15, no. 9, pp. e0239479, 2020, ISSN: 1932-6203.
@article{pmid32966310,
title = {Usability of graphene oxide as a mycotoxin binder: In vitro study},
author = {Pavel Horky and Eva Venusova and Tereza Aulichova and Andrea Ridoskova and Jiri Skladanka and Sylvie Skalickova},
doi = {10.1371/journal.pone.0239479},
issn = {1932-6203},
year = {2020},
date = {2020-01-01},
journal = {PLoS One},
volume = {15},
number = {9},
pages = {e0239479},
abstract = {Mycotoxin management in agriculture is an essential challenge for maintaining the health of both animals and humans. Choosing the right adsorbent is still a question for many breeders and an important criterion for feed manufacturers. New adsorbents are still being sought. Graphene oxide is a promising material in the field of nanotechnology, which excels in its adsorption properties. Presented in vitro study investigates graphene oxide for the binding of mycotoxins from crushed wheat. The results show that graphene oxide has an adsorption capacity for aflatoxin 0.045 mg/g, zearalenone 0.53 mg/g and deoxynivalenol 1.69 mg/g at 37° C. In vitro simulation of crushed wheat digestion showed rapid adsorption during the gastric phase. Of the minerals, Mg, Cu and Zn were the most adsorbed. The applied dose of graphene oxide of 10 mg/g caused only a slight inhibition of the digestive enzymes α-amylase and trypsin compared to pepsin and gastric lipase. In vitro results indicated the suitability of graphene oxide in the adsorption of the aflatoxin, zearalenone and deoxynivalenol.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mycotoxin management in agriculture is an essential challenge for maintaining the health of both animals and humans. Choosing the right adsorbent is still a question for many breeders and an important criterion for feed manufacturers. New adsorbents are still being sought. Graphene oxide is a promising material in the field of nanotechnology, which excels in its adsorption properties. Presented in vitro study investigates graphene oxide for the binding of mycotoxins from crushed wheat. The results show that graphene oxide has an adsorption capacity for aflatoxin 0.045 mg/g, zearalenone 0.53 mg/g and deoxynivalenol 1.69 mg/g at 37° C. In vitro simulation of crushed wheat digestion showed rapid adsorption during the gastric phase. Of the minerals, Mg, Cu and Zn were the most adsorbed. The applied dose of graphene oxide of 10 mg/g caused only a slight inhibition of the digestive enzymes α-amylase and trypsin compared to pepsin and gastric lipase. In vitro results indicated the suitability of graphene oxide in the adsorption of the aflatoxin, zearalenone and deoxynivalenol.
Pelcová, Pavlína; Ridošková, Andrea; Hrachovinová, Jana; Grmela, Jan
Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination Journal Article
In: Environ Toxicol Chem, vol. 39, no. 9, pp. 1670–1677, 2020, ISSN: 1552-8618.
@article{pmid32516439,
title = {Fractionation Analysis of Mercury in Soils: A Comparison of Three Techniques for Bioavailable Mercury Fraction Determination},
author = {Pavlína Pelcová and Andrea Ridošková and Jana Hrachovinová and Jan Grmela},
doi = {10.1002/etc.4797},
issn = {1552-8618},
year = {2020},
date = {2020-01-01},
journal = {Environ Toxicol Chem},
volume = {39},
number = {9},
pages = {1670--1677},
abstract = {Knowledge of the fractionation of mercury in soils in the vicinity of abandoned cinnabar mines is essential for assessing the usability of soils for the cultivation of agriculturally important crops. Two different sequential extraction methods and the technique of diffusive gradients in thin films (DGT) were applied and compared for fractionation of mercury in soils from mercury-contaminated sites intended for farming purposes. The mercury found in these soils was primarily in the form of mercury sulfide (58.6-83.9%), followed by 6.7 to 15.4% of organically bound mercury and 2.9 to 23.2% of elemental mercury. Up to 10.3% of labile mercury species were determined by both sequential extraction methods in these soils. However, only 0.01 to 0.13% of mercury was determined as a bioavailable fraction using the DGT technique. Both sequential extraction methods tested for the fractionation analysis of mercury in contaminated soils were in excellent agreement. The content of the mobile (labile) mercury determined by the sequential extraction methods was statistically significantly higher (p < 0.0001) than the content of bioavailable mercury determined by the DGT technique. Environ Toxicol Chem 2020;39:1670-1677. © 2020 SETAC.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Knowledge of the fractionation of mercury in soils in the vicinity of abandoned cinnabar mines is essential for assessing the usability of soils for the cultivation of agriculturally important crops. Two different sequential extraction methods and the technique of diffusive gradients in thin films (DGT) were applied and compared for fractionation of mercury in soils from mercury-contaminated sites intended for farming purposes. The mercury found in these soils was primarily in the form of mercury sulfide (58.6-83.9%), followed by 6.7 to 15.4% of organically bound mercury and 2.9 to 23.2% of elemental mercury. Up to 10.3% of labile mercury species were determined by both sequential extraction methods in these soils. However, only 0.01 to 0.13% of mercury was determined as a bioavailable fraction using the DGT technique. Both sequential extraction methods tested for the fractionation analysis of mercury in contaminated soils were in excellent agreement. The content of the mobile (labile) mercury determined by the sequential extraction methods was statistically significantly higher (p < 0.0001) than the content of bioavailable mercury determined by the DGT technique. Environ Toxicol Chem 2020;39:1670-1677. © 2020 SETAC.
2019
Pelcová, Pavlína; Zouharová, Iveta; Ridošková, Andrea; Smolíková, Vendula
In: Chemosphere, vol. 234, pp. 373–378, 2019, ISSN: 1879-1298.
@article{pmid31228839,
title = {Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model},
author = {Pavlína Pelcová and Iveta Zouharová and Andrea Ridošková and Vendula Smolíková},
doi = {10.1016/j.chemosphere.2019.06.076},
issn = {1879-1298},
year = {2019},
date = {2019-11-01},
journal = {Chemosphere},
volume = {234},
pages = {373--378},
abstract = {The diffusive gradients in thin films technique (DGT) was used for the determination of bioavailable mercury in urban soils, and results were compared to the mercury accumulation by Pisum sativum L. (pea) parts (leaf, root, stem, blossom, legume, and green seed). The total mercury concentration in soil samples was ranged between 0.084 and 0.326 mg kg. The soil solutions contained 0.15%-0.20% of mercury present in soils. In the soil solution, 2.21%-3.45% of mercury was available for DGT units. The highest mercury content was determined in the leaf and root of the pea plant, and the lowest in the consumable part of the pea plant (green seed). Mercury concentration in the parts of the pea plant increased over time, alongside the growth of the plant. The effect of acid precipitation on mercury bioavailability was statistically non-significant (p = 0.53). Significant and positive correlations were found between mercury flux into DGT unit, and mercury flux into the root (r = 0.989), leaf (r = 0.985), and stem (r = 0.904) of the pea plant. The obtained results suggest that the DGT method could be used for description of the uptake of mercury by pea plant parts in non-contaminated and slightly contaminated soils.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The diffusive gradients in thin films technique (DGT) was used for the determination of bioavailable mercury in urban soils, and results were compared to the mercury accumulation by Pisum sativum L. (pea) parts (leaf, root, stem, blossom, legume, and green seed). The total mercury concentration in soil samples was ranged between 0.084 and 0.326 mg kg. The soil solutions contained 0.15%-0.20% of mercury present in soils. In the soil solution, 2.21%-3.45% of mercury was available for DGT units. The highest mercury content was determined in the leaf and root of the pea plant, and the lowest in the consumable part of the pea plant (green seed). Mercury concentration in the parts of the pea plant increased over time, alongside the growth of the plant. The effect of acid precipitation on mercury bioavailability was statistically non-significant (p = 0.53). Significant and positive correlations were found between mercury flux into DGT unit, and mercury flux into the root (r = 0.989), leaf (r = 0.985), and stem (r = 0.904) of the pea plant. The obtained results suggest that the DGT method could be used for description of the uptake of mercury by pea plant parts in non-contaminated and slightly contaminated soils.
Brtnický, Martin; Pecina, Václav; Hladký, Jan; Radziemska, Maja; Koudelková, Zuzana; Klimánek, Martin; Richtera, Lukáš; Adamcová, Dana; Elbl, Jakub; Galiová, Michaela Vašinová; Baláková, Ludmila; Kynický, Jindřich; Smolíková, Vendula; Houška, Jakub; Vaverková, Magdalena Daria
Assessment of phytotoxicity, environmental and health risks of historical urban park soils Journal Article
In: Chemosphere, vol. 220, pp. 678–686, 2019, ISSN: 1879-1298.
@article{pmid30605810,
title = {Assessment of phytotoxicity, environmental and health risks of historical urban park soils},
author = {Martin Brtnický and Václav Pecina and Jan Hladký and Maja Radziemska and Zuzana Koudelková and Martin Klimánek and Lukáš Richtera and Dana Adamcová and Jakub Elbl and Michaela Vašinová Galiová and Ludmila Baláková and Jindřich Kynický and Vendula Smolíková and Jakub Houška and Magdalena Daria Vaverková},
doi = {10.1016/j.chemosphere.2018.12.188},
issn = {1879-1298},
year = {2019},
date = {2019-04-01},
journal = {Chemosphere},
volume = {220},
pages = {678--686},
abstract = {Urban soil areas can be contaminated with potentially dangerous heavy metals (HM), which might have got there by means of the human activity. The aim of the present study was to determine the contamination level of the city park soils and its impact on urban ecosystem. The indices assessing soil contamination such as Enrichment Factor (EF), Geoaccumulation Index (Igeo), Nemerow Pollution Index (IPI), and indices assessing health risks, namely Hazard Index (HI) and Carcinogenic Risk (CR), have been calculated. Furthermore, the phytotoxic effect of the soil samples has been determined. The soil contains in average 58.6 mg/kg Zn, 0.3 mg/kg Cd, 27.2 mg/kg Pb and 16.6 mg/kg Cu. Based on EF index, it has been confirmed that the increased amounts of Zn, Cd and Pb in the soil are of the anthropogenic origin. The soil may be classified as moderately to strongly polluted in the case of Zn and Pb according to Igeo. Nevertheless, soil contamination in the park is at a safe level as per IPI Based on HI and CR indices, it is possible to state that the soil in the park does not pose any health risks. Subject to the outcomes of the toxicity test, the concentrations of HMs found out in the soils are not inhibitory for plants.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Urban soil areas can be contaminated with potentially dangerous heavy metals (HM), which might have got there by means of the human activity. The aim of the present study was to determine the contamination level of the city park soils and its impact on urban ecosystem. The indices assessing soil contamination such as Enrichment Factor (EF), Geoaccumulation Index (Igeo), Nemerow Pollution Index (IPI), and indices assessing health risks, namely Hazard Index (HI) and Carcinogenic Risk (CR), have been calculated. Furthermore, the phytotoxic effect of the soil samples has been determined. The soil contains in average 58.6 mg/kg Zn, 0.3 mg/kg Cd, 27.2 mg/kg Pb and 16.6 mg/kg Cu. Based on EF index, it has been confirmed that the increased amounts of Zn, Cd and Pb in the soil are of the anthropogenic origin. The soil may be classified as moderately to strongly polluted in the case of Zn and Pb according to Igeo. Nevertheless, soil contamination in the park is at a safe level as per IPI Based on HI and CR indices, it is possible to state that the soil in the park does not pose any health risks. Subject to the outcomes of the toxicity test, the concentrations of HMs found out in the soils are not inhibitory for plants.
Ridošková, Andrea; Pelfrêne, Aurélie; Douay, Francis; Pelcová, Pavlína; Smolíková, Vendula; Adam, Vojtěch
In: Environ Toxicol Chem, vol. 38, no. 2, pp. 321–328, 2019, ISSN: 1552-8618.
@article{pmid30620786,
title = {Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus},
author = {Andrea Ridošková and Aurélie Pelfrêne and Francis Douay and Pavlína Pelcová and Vendula Smolíková and Vojtěch Adam},
doi = {10.1002/etc.4318},
issn = {1552-8618},
year = {2019},
date = {2019-01-01},
journal = {Environ Toxicol Chem},
volume = {38},
number = {2},
pages = {321--328},
abstract = {We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion-exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg species were found in the metal-contaminated agricultural soils, as determined by a high-performance liquid chromatography-cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems (7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. © 2018 SETAC.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion-exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg species were found in the metal-contaminated agricultural soils, as determined by a high-performance liquid chromatography-cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems (7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. © 2018 SETAC.
Horky, Pavel; Skalickova, Sylvie; Urbankova, Lenka; Baholet, Daria; Kociova, Silvia; Bytesnikova, Zuzana; Kabourkova, Eliska; Lackova, Zuzana; Cernei, Natalia; Gagic, Milica; Milosavljevic, Vedran; Smolikova, Vendula; Vaclavkova, Eva; Nevrkla, Pavel; Knot, Pavel; Krystofova, Olga; Hynek, David; Kopel, Pavel; Skladanka, Jiri; Adam, Vojtech; Smerkova, Kristyna
Zinc phosphate-based nanoparticles as a novel antibacterial agent: study on rats after dietary exposure Journal Article
In: J Anim Sci Biotechnol, vol. 10, pp. 17, 2019, ISSN: 1674-9782.
@article{pmid30805185,
title = {Zinc phosphate-based nanoparticles as a novel antibacterial agent: study on rats after dietary exposure},
author = {Pavel Horky and Sylvie Skalickova and Lenka Urbankova and Daria Baholet and Silvia Kociova and Zuzana Bytesnikova and Eliska Kabourkova and Zuzana Lackova and Natalia Cernei and Milica Gagic and Vedran Milosavljevic and Vendula Smolikova and Eva Vaclavkova and Pavel Nevrkla and Pavel Knot and Olga Krystofova and David Hynek and Pavel Kopel and Jiri Skladanka and Vojtech Adam and Kristyna Smerkova},
doi = {10.1186/s40104-019-0319-8},
issn = {1674-9782},
year = {2019},
date = {2019-01-01},
urldate = {2019-01-01},
journal = {J Anim Sci Biotechnol},
volume = {10},
pages = {17},
abstract = {BACKGROUND: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
Horky, Pavel; Skalickova, Sylvie; Urbankova, Lenka; Baholet, Daria; Kociova, Silvia; Bytesnikova, Zuzana; Kabourkova, Eliska; Lackova, Zuzana; Cernei, Natalia; Gagic, Milica; Milosavljevic, Vedran; Smolikova, Vendula; Vaclavkova, Eva; Nevrkla, Pavel; Knot, Pavel; Krystofova, Olga; Hynek, David; Kopel, Pavel; Skladanka, Jiri; Adam, Vojtech; Smerkova, Kristyna
Zinc phosphate-based nanoparticles as a novel antibacterial agent: study on rats after dietary exposure Journal Article
In: J Anim Sci Biotechnol, vol. 10, pp. 17, 2019, ISSN: 1674-9782.
@article{pmid30805185b,
title = {Zinc phosphate-based nanoparticles as a novel antibacterial agent: study on rats after dietary exposure},
author = {Pavel Horky and Sylvie Skalickova and Lenka Urbankova and Daria Baholet and Silvia Kociova and Zuzana Bytesnikova and Eliska Kabourkova and Zuzana Lackova and Natalia Cernei and Milica Gagic and Vedran Milosavljevic and Vendula Smolikova and Eva Vaclavkova and Pavel Nevrkla and Pavel Knot and Olga Krystofova and David Hynek and Pavel Kopel and Jiri Skladanka and Vojtech Adam and Kristyna Smerkova},
doi = {10.1186/s40104-019-0319-8},
issn = {1674-9782},
year = {2019},
date = {2019-01-01},
journal = {J Anim Sci Biotechnol},
volume = {10},
pages = {17},
abstract = {BACKGROUND: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
RESULTS: Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest inhibitory effect of our NPs was observed against (inhibitory concentration values, IC, ranged from 0.5 to 1.6 mmol/L) followed by (IC 0.8-1.5 mmol/L). In contrast, methicillin resistant (IC 1.2-4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher ( < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased ( < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more ( < 0.05).
CONCLUSIONS: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
Ridošková, Andrea; Pelfrêne, Aurélie; Douay, Francis; Pelcová, Pavlína; Smolíková, Vendula; Adam, Vojtěch
In: Environ Toxicol Chem, vol. 38, no. 2, pp. 321–328, 2019, ISSN: 1552-8618.
@article{pmid30620786b,
title = {Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus},
author = {Andrea Ridošková and Aurélie Pelfrêne and Francis Douay and Pavlína Pelcová and Vendula Smolíková and Vojtěch Adam},
doi = {10.1002/etc.4318},
issn = {1552-8618},
year = {2019},
date = {2019-01-01},
journal = {Environ Toxicol Chem},
volume = {38},
number = {2},
pages = {321--328},
abstract = {We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion-exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg species were found in the metal-contaminated agricultural soils, as determined by a high-performance liquid chromatography-cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems (7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. © 2018 SETAC.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
We assessed the relationship between the diffusive gradient in thin film (DGT) technique using the new ion-exchange resin Ambersep GT74 and the uptake of mercury (Hg) by a model plant cultivated on metal-contaminated agricultural soils under greenhouse conditions. Based on the total Hg content, 0.37 to 1.17% of the Hg passed to the soil porewater from the solid phase, and 2.18 to 9.18% of the Hg is DGT-available. These results were confirmed by calculating the R value (the ratio of the concentrations of bioavailable Hg measured by DGT and soil solution), which illustrated the strong bonding of Hg to the solid phase of soil and its extremely low mobility. Only inorganic Hg species were found in the metal-contaminated agricultural soils, as determined by a high-performance liquid chromatography-cold vapor atomic fluorescence spectrometry speciation analysis. The Hg was distributed in Miscanthus × giganteus organs in the following order for all sampling sites: roots (55-82%) >> leaves (8-27%) > stems (7-16%) > rhizomes (4-7%). Environ Toxicol Chem 2019;38:321-328. © 2018 SETAC.
2018
Pelcová, Pavlína; Vičarová, Petra; Dočekalová, Hana; Poštulková, Eva; Kopp, Radovan; Mareš, Jan; Smolíková, Vendula
In: Chemosphere, vol. 211, pp. 1109–1112, 2018, ISSN: 1879-1298.
@article{pmid30223326,
title = {The prediction of mercury bioavailability for common carp (Cyprinus carpio L.) using the DGT technique in the presence of chloride ions and humic acid},
author = {Pavlína Pelcová and Petra Vičarová and Hana Dočekalová and Eva Poštulková and Radovan Kopp and Jan Mareš and Vendula Smolíková},
doi = {10.1016/j.chemosphere.2018.07.202},
issn = {1879-1298},
year = {2018},
date = {2018-11-01},
journal = {Chemosphere},
volume = {211},
pages = {1109--1112},
abstract = {The ability of the DGT technique to predict Hg bioavailability for input tissues (skin, gills, and scales) of common carp in the presence of chloride ions and humic acid (HA) was evaluated. The mercury accumulation by the DGT units and input tissues of carp decreased with an increasing concentration of chloride ions (29-180 mg L) and HA (0-5 mg L). In the presence of chloride ions and HA, statistically significant correlations (Pearson's correlation coefficients 0.731-0.954) were determined between the rate of mercury accumulation by input tissues of carp and the rate of mercury accumulation by the DGT units. The laboratory experiments suggest the possibility of using the DGT technique for predicting the mercury bioaccumulation in natural aquatic ecosystems instead of commonly used input tissues of fish.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The ability of the DGT technique to predict Hg bioavailability for input tissues (skin, gills, and scales) of common carp in the presence of chloride ions and humic acid (HA) was evaluated. The mercury accumulation by the DGT units and input tissues of carp decreased with an increasing concentration of chloride ions (29-180 mg L) and HA (0-5 mg L). In the presence of chloride ions and HA, statistically significant correlations (Pearson's correlation coefficients 0.731-0.954) were determined between the rate of mercury accumulation by input tissues of carp and the rate of mercury accumulation by the DGT units. The laboratory experiments suggest the possibility of using the DGT technique for predicting the mercury bioaccumulation in natural aquatic ecosystems instead of commonly used input tissues of fish.
Urbankova, Lenka; Horky, Pavel; Skladanka, Jiri; Pribilova, Magdalena; Smolikova, Vendula; Nevrkla, Pavel; Cernei, Natalia; Lackova, Zuzana; Hedbavny, Josef; Ridoskova, Andrea; Adam, Vojtech; Kopel, Pavel
Antioxidant status of rats' blood and liver affected by sodium selenite and selenium nanoparticles Journal Article
In: PeerJ, vol. 6, pp. e4862, 2018, ISSN: 2167-8359.
@article{pmid29868274,
title = {Antioxidant status of rats' blood and liver affected by sodium selenite and selenium nanoparticles},
author = {Lenka Urbankova and Pavel Horky and Jiri Skladanka and Magdalena Pribilova and Vendula Smolikova and Pavel Nevrkla and Natalia Cernei and Zuzana Lackova and Josef Hedbavny and Andrea Ridoskova and Vojtech Adam and Pavel Kopel},
doi = {10.7717/peerj.4862},
issn = {2167-8359},
year = {2018},
date = {2018-01-01},
journal = {PeerJ},
volume = {6},
pages = {e4862},
abstract = {BACKGROUND: Selenium is an essential element; however, at higher doses, it can be toxic. Therefore, alternative nanotechnological solutions are required to overcome toxicological issues, rather than conventional alternatives. Nanoparticles show new and promising properties that may be able to suppress toxicity while maintaining the positive effects of selenium on an organism. The aim of the experiment was to determine the influence of sodium selenite and selenium nanoparticles (SeNPs) on the antioxidant status of rats.
METHODS: The males of the outbreed rat strain Wistar albino were selected as a model organism. Animals were fed different forms of selenium. The control group was given a mixture without selenium addition, whereas other groups were fed a mixture containing sodium selenite, Se-49, and Se-100 SeNPs respectively. The duration of the trial was 30 days.
RESULTS: Analysis of blood and liver was performed where the concentration of reduced (GSH) and oxidised (GSSG) glutathione, and total selenium content were measured. In the liver, a significant reduction in GSSG was found for all experiment groups. Blood samples showed a significant reduction in GSH and an increase in GSSG.
DISCUSSION: These results show that SeNPs may be an alternative to dietary selenium for animal organisms.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
BACKGROUND: Selenium is an essential element; however, at higher doses, it can be toxic. Therefore, alternative nanotechnological solutions are required to overcome toxicological issues, rather than conventional alternatives. Nanoparticles show new and promising properties that may be able to suppress toxicity while maintaining the positive effects of selenium on an organism. The aim of the experiment was to determine the influence of sodium selenite and selenium nanoparticles (SeNPs) on the antioxidant status of rats.
METHODS: The males of the outbreed rat strain Wistar albino were selected as a model organism. Animals were fed different forms of selenium. The control group was given a mixture without selenium addition, whereas other groups were fed a mixture containing sodium selenite, Se-49, and Se-100 SeNPs respectively. The duration of the trial was 30 days.
RESULTS: Analysis of blood and liver was performed where the concentration of reduced (GSH) and oxidised (GSSG) glutathione, and total selenium content were measured. In the liver, a significant reduction in GSSG was found for all experiment groups. Blood samples showed a significant reduction in GSH and an increase in GSSG.
DISCUSSION: These results show that SeNPs may be an alternative to dietary selenium for animal organisms.
METHODS: The males of the outbreed rat strain Wistar albino were selected as a model organism. Animals were fed different forms of selenium. The control group was given a mixture without selenium addition, whereas other groups were fed a mixture containing sodium selenite, Se-49, and Se-100 SeNPs respectively. The duration of the trial was 30 days.
RESULTS: Analysis of blood and liver was performed where the concentration of reduced (GSH) and oxidised (GSSG) glutathione, and total selenium content were measured. In the liver, a significant reduction in GSSG was found for all experiment groups. Blood samples showed a significant reduction in GSH and an increase in GSSG.
DISCUSSION: These results show that SeNPs may be an alternative to dietary selenium for animal organisms.
2017
Pelcová, Pavlína; Vičarová, Petra; Ridošková, Andrea; Dočekalová, Hana; Kopp, Radovan; Mareš, Jan; Poštulková, Eva
Prediction of mercury bioavailability to common carp (Cyprinus carpio L.) using the diffusive gradient in thin film technique Journal Article
In: Chemosphere, vol. 187, pp. 181–187, 2017, ISSN: 1879-1298.
@article{pmid28846974,
title = {Prediction of mercury bioavailability to common carp (Cyprinus carpio L.) using the diffusive gradient in thin film technique},
author = {Pavlína Pelcová and Petra Vičarová and Andrea Ridošková and Hana Dočekalová and Radovan Kopp and Jan Mareš and Eva Poštulková},
doi = {10.1016/j.chemosphere.2017.08.097},
issn = {1879-1298},
year = {2017},
date = {2017-11-01},
journal = {Chemosphere},
volume = {187},
pages = {181--187},
abstract = {The mercury bioaccumulation by common carp (Cyprinus carpio L.) tissues (gills, skin, eyes, scales, muscle, brain, kidneys, liver, and spleen) and the capability of the diffusive gradient in thin film (DGT) technique to predict bioavailability of mercury for individual carp's tissues were evaluated. Carp and DGT units were exposed to increasing concentrations of mercury (Hg: 0 μg L, 0.5 μg L, 1.5 μg L and 3.0 μg L) in fish tanks for 14 days. In the uncontaminated fish group, the highest mercury concentration was determined in the muscle tissues and, in fish groups exposed to mercury, the highest mercury concentration was determined in the detoxification (kidneys) and input (gills) organs. A strong and positive correlation between the rate of mercury uptake by the DGT technique and the rate of mercury accumulation by fish tissues (gills, skin, scales, and eyes) was observed.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The mercury bioaccumulation by common carp (Cyprinus carpio L.) tissues (gills, skin, eyes, scales, muscle, brain, kidneys, liver, and spleen) and the capability of the diffusive gradient in thin film (DGT) technique to predict bioavailability of mercury for individual carp's tissues were evaluated. Carp and DGT units were exposed to increasing concentrations of mercury (Hg: 0 μg L, 0.5 μg L, 1.5 μg L and 3.0 μg L) in fish tanks for 14 days. In the uncontaminated fish group, the highest mercury concentration was determined in the muscle tissues and, in fish groups exposed to mercury, the highest mercury concentration was determined in the detoxification (kidneys) and input (gills) organs. A strong and positive correlation between the rate of mercury uptake by the DGT technique and the rate of mercury accumulation by fish tissues (gills, skin, scales, and eyes) was observed.
Adamcová, Dana; Radziemska, Maja; Ridošková, Andrea; Bartoň, Stanislav; Pelcová, Pavlína; Elbl, Jakub; Kynický, Jindřich; Brtnický, Martin; Vaverková, Magdalena Daria
Environmental assessment of the effects of a municipal landfill on the content and distribution of heavy metals in Tanacetum vulgare L Journal Article
In: Chemosphere, vol. 185, pp. 1011–1018, 2017, ISSN: 1879-1298.
@article{pmid28753902,
title = {Environmental assessment of the effects of a municipal landfill on the content and distribution of heavy metals in Tanacetum vulgare L},
author = {Dana Adamcová and Maja Radziemska and Andrea Ridošková and Stanislav Bartoň and Pavlína Pelcová and Jakub Elbl and Jindřich Kynický and Martin Brtnický and Magdalena Daria Vaverková},
doi = {10.1016/j.chemosphere.2017.07.060},
issn = {1879-1298},
year = {2017},
date = {2017-10-01},
journal = {Chemosphere},
volume = {185},
pages = {1011--1018},
abstract = {Heavy metal pollution is an important concern because of its potential to affect human health. This study was conducted to analyze plants growing on a landfill body and in its surroundings to determine their potential for heavy metal accumulation. In addition, the enrichment coefficient (EC) for the plant/soil system was used for determining the environmental contamination from a landfill in terms of heavy metal accumulation. The samples were taken in 2013-2014. Of the analyzed metals, iron achieved the highest values in the samples, i.e. - stalk (103.4-6564.6 mg/kg DM), roots (6563.6-33,036.6 mg/kg DM), leaf (535.1-11,275 mg/kg DM) and soil (12,389-39,381.9 mg/kg DM). The highest concentrations were determined in 2013 for Fe, Mn and Zn. Iron achieved the highest concentrations in the years 2013-2014. Next, EC values were then calculated, with the highest noted for Cd. Cd, as well as Cr, Ni and Zn are accumulated mostly in the leaves, whereas Co, Cu, Fe, Hg, Mn and Pb are accumulated mainly in the roots of T. vulgare.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Heavy metal pollution is an important concern because of its potential to affect human health. This study was conducted to analyze plants growing on a landfill body and in its surroundings to determine their potential for heavy metal accumulation. In addition, the enrichment coefficient (EC) for the plant/soil system was used for determining the environmental contamination from a landfill in terms of heavy metal accumulation. The samples were taken in 2013-2014. Of the analyzed metals, iron achieved the highest values in the samples, i.e. - stalk (103.4-6564.6 mg/kg DM), roots (6563.6-33,036.6 mg/kg DM), leaf (535.1-11,275 mg/kg DM) and soil (12,389-39,381.9 mg/kg DM). The highest concentrations were determined in 2013 for Fe, Mn and Zn. Iron achieved the highest concentrations in the years 2013-2014. Next, EC values were then calculated, with the highest noted for Cd. Cd, as well as Cr, Ni and Zn are accumulated mostly in the leaves, whereas Co, Cu, Fe, Hg, Mn and Pb are accumulated mainly in the roots of T. vulgare.
Buchtelova, Hana; Dostalova, Simona; Michalek, Petr; Krizkova, Sona; Strmiska, Vladislav; Kopel, Pavel; Hynek, David; Richtera, Lukas; Ridoskova, Andrea; Adam, Pavlina; Kynicky, Jindrich; Brtnicky, Martin; Heger, Zbynek; Adam, Vojtech
Size-related cytotoxicological aspects of polyvinylpyrrolidone-capped platinum nanoparticles Journal Article
In: Food Chem Toxicol, vol. 105, pp. 337–346, 2017, ISSN: 1873-6351.
@article{pmid28465190,
title = {Size-related cytotoxicological aspects of polyvinylpyrrolidone-capped platinum nanoparticles},
author = {Hana Buchtelova and Simona Dostalova and Petr Michalek and Sona Krizkova and Vladislav Strmiska and Pavel Kopel and David Hynek and Lukas Richtera and Andrea Ridoskova and Pavlina Adam and Jindrich Kynicky and Martin Brtnicky and Zbynek Heger and Vojtech Adam},
doi = {10.1016/j.fct.2017.04.043},
issn = {1873-6351},
year = {2017},
date = {2017-07-01},
journal = {Food Chem Toxicol},
volume = {105},
pages = {337--346},
abstract = {The nanotechnological concept is based on size-dependent properties of particles in the 1-100 nm range. Nevertheless, the connection between their size and effect is still not clear. Thus, we focused on reductive colloidal synthesis, characterization and biological testing of Pt nanoparticles (PtNPs) capped with biocompatible polymer polyvinylpyrrolidone (PVP). Synthesized PtNPs were of 3 different primary sizes (approx. ∼10; ∼14 and > 20 nm) and demonstrated exceptional haemocompatibility. In vitro treatment of three different types of malignant cells (prostate - LNCaP, breast - MDA-MB-231 and neuroblastoma - GI-ME-N) revealed that even marginal differences in PtNPs diameter resulted in changes in their cytotoxicity. The highest cytotoxicity was observed using the smallest PtNPs-10, where 24IC was lower (3.1-6.2 μg/mL) than for cisplatin (8.1-19.8 μg/mL). In contrast to MDA-MB-231 and LNCaP cells, in GI-ME-N cells PtNPs caused noticeable changes in their cellular structure without influencing their viability. Post-exposure analyses revealed that PtNPs-29 and PtNPs-40 were capable of forming considerably higher amount of reactive oxygen species with consequent stimulation of expression of metallothionein (MT1/2 and MT3), at both mRNA and protein level. Overall, our pilot study demonstrates that in the nanoscaled world even the smallest differences can have crucial biological effect.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The nanotechnological concept is based on size-dependent properties of particles in the 1-100 nm range. Nevertheless, the connection between their size and effect is still not clear. Thus, we focused on reductive colloidal synthesis, characterization and biological testing of Pt nanoparticles (PtNPs) capped with biocompatible polymer polyvinylpyrrolidone (PVP). Synthesized PtNPs were of 3 different primary sizes (approx. ∼10; ∼14 and > 20 nm) and demonstrated exceptional haemocompatibility. In vitro treatment of three different types of malignant cells (prostate - LNCaP, breast - MDA-MB-231 and neuroblastoma - GI-ME-N) revealed that even marginal differences in PtNPs diameter resulted in changes in their cytotoxicity. The highest cytotoxicity was observed using the smallest PtNPs-10, where 24IC was lower (3.1-6.2 μg/mL) than for cisplatin (8.1-19.8 μg/mL). In contrast to MDA-MB-231 and LNCaP cells, in GI-ME-N cells PtNPs caused noticeable changes in their cellular structure without influencing their viability. Post-exposure analyses revealed that PtNPs-29 and PtNPs-40 were capable of forming considerably higher amount of reactive oxygen species with consequent stimulation of expression of metallothionein (MT1/2 and MT3), at both mRNA and protein level. Overall, our pilot study demonstrates that in the nanoscaled world even the smallest differences can have crucial biological effect.
2016
Vičarová, Petra; Dočekalová, Hana; Ridošková, Andrea; Pelcová, Pavlina
Heavy metals in the common carp (Cyprinus carpio L.) from three reservoirs in the Czech Republic Journal Article
In: Czech Journal of Food Sciences, vol. 34, iss. 5, pp. 422-428, 2016.
@article{nokey,
title = {Heavy metals in the common carp (Cyprinus carpio L.) from three reservoirs in the Czech Republic},
author = {Petra Vičarová and Hana Dočekalová and Andrea Ridošková and Pavlina Pelcová},
doi = {https://doi.org/10.17221/100/2016-CJFS},
year = {2016},
date = {2016-11-01},
journal = {Czech Journal of Food Sciences},
volume = {34},
issue = {5},
pages = {422-428},
abstract = {Toxic metals (cadmium, lead, and mercury) in the tissues of common carp fished from three reservoirs (Pilská, Domaninský, and Matějovský) in the Bohemian-Moravian Highlands were measured in the period from April 2013 to September 2014 and the risk of consuming the fish from these localities was evaluated. During this project 25 fish specimens from each locality were analysed. The concentration of metals in muscle tissue and liver was determined by atomic absorption spectrometry. A variation in the content of heavy metals in fish samples and fish tissues was demonstrated and discussed. Higher concentrations of cadmium in the fish tissue were found in 50 samples of carp liver. Because the fish liver is not commonly consumed, common carp from the Moravian-Moravian Highlands does not pose any health risk for consumers. Concentrations of the other monitored toxic metals in fish tissue were low and were complying with the maximum residue limit in all cases.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Toxic metals (cadmium, lead, and mercury) in the tissues of common carp fished from three reservoirs (Pilská, Domaninský, and Matějovský) in the Bohemian-Moravian Highlands were measured in the period from April 2013 to September 2014 and the risk of consuming the fish from these localities was evaluated. During this project 25 fish specimens from each locality were analysed. The concentration of metals in muscle tissue and liver was determined by atomic absorption spectrometry. A variation in the content of heavy metals in fish samples and fish tissues was demonstrated and discussed. Higher concentrations of cadmium in the fish tissue were found in 50 samples of carp liver. Because the fish liver is not commonly consumed, common carp from the Moravian-Moravian Highlands does not pose any health risk for consumers. Concentrations of the other monitored toxic metals in fish tissue were low and were complying with the maximum residue limit in all cases.