Laboratory of synthesis and characterization of nanomaterials

The research interest of this group is focused on synthesis and nanomaterial characterization which are further use for bioapplications or in electrochemistry for the detection of target analytes. Materials that we synthesize can be divide into metal, magnetic, carbon nanoparticles, polymeric nanoparticles, metal-organic frameworks, and liposomes. We collaborate with different research groups to test our as-prepared materials on bacteria, carcinoma cells, algae, fish, or mammals.

Laboratoř syntézy a charakterizace nanomateriálů

Our research interest

  • Synthesis of nanomaterials
  • Characterization of biological samples
  • Nanomaterial characterization
  • Mapping of surface materials by electrochemical scanning microscope
  • Elemental analysis of nanomaterials and biological samples



  • Dynamic light scattering (Malvern Panalytical)
  • X-ray fluorescence spectrometer (XEPOS)
  • Scanning electron microscope (TESCAN)
  • Fourier transform infrared spectrometer with Cryo mode (Bruker)
  • Elemental analyzer CHNS/O (Thermo Fisher)
  • Microbalance (Mettler Toledo)
  • … and other standard equipment – oven, balance, magnetic stirrers, pH meter…

Possibility of using equipment at CEITEC – atomic force microscope (AFM), confocal Raman microscope, transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDX).


Ing. Zuzana Bytešníková, Ph.D.

Head – Laboratory of synthesis and characterization of nanomaterials
Assistant Professor

Phone: +420 545 133 249
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N2016
ORCID: 0000-0003-1648-8517

Web of Science ResearchID: P-5035-2018
Scopus ID: 57201821000

Team members

  • Ing. Pavel Švec, Ph.D.
  • Ing. Jana Pekárková, Ph.D.
  • Soumajit Mukherjee, MSc.

Topics of Ph.D. theses

  • Nanomaterials for plant protection
  • Nanomaterials for application in agricultural practice
  • Carbon nanomaterials and their composites for bioapplications
  • Optional topic


  • ASHRAFI, A.; BYTESNIKOVA, Z.; BAREK, J.; RICHTERA, L.; ADAM, V. A Critical Comparison of Natural Enzymes and Nanozymes in Biosensing and Bioassays. Biosens. Bioelectron.2021, vol. 192. p. 1-18. ISSN 0956-5663. IF 10.618
  • PECENKA, J.; BYTESNIKOVA, Z.; KISS, M.; PENAZOVA, E.; BARANEK, M.; EICHMEIER, A.; TEKIELSKA, D.; RICHTERA, L.; POKLUDA, R.; ADAM, V. Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment. Mater. Today Commun.2021, vol. 27. p. 1-8. ISSN 2352-4928. IF 3.383
  • JANOVA, A.; KOLACKOVA, I.; BYTESNIKOVA, Z.; CAPAL, P.; CHALOUPSKY, P.; SVEC, P.; RIDOSKOVA, A.; CERNEI, N.; KLEJDUS, B.; RICHTERA, L.; ADAM, V.; HUSKA, D. New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites. Algal Res., 2021, vol., p. 1-11. ISSN 2211-9264. IF 4.401
  • Bytesnikova Z, Adam V, Richtera L (2021) Graphene oxide as a novel tool for mycotoxin removal. Food Control 121: 1-9. clanek IF 4.258
  • Mukherjee, S., Bytesnikova, Z., Ashrafi, A., Adam, V., Richtera, L. (2020). Graphene Oxide as a Nanocarrier for Biochemical Molecules: Current Understanding and Trends. Processes, 8(12), 1636. clanek IF 2.735
  • Sedlackova E, Bytesnikova Z, Birgusova E, Svec P, Ashrafi AM, Estrela P, Richtera L (2020) Label-free DNA biosensor using modified reduced graphene oxide platform as a DNA methylation assay. Materials 13: 1-12. clanek IF 3.057
  • Mukherjee A, Ashrafi AM, Svec P, Richtera L, Adam V, Pribyl J, Brtnicky M, Kynicky J (2020) The effect of synthesis procedure on hydrogen peroxidase-like catalytic activity of iron oxide magnetic particles. Appl Sci-Basel 10: 1-12. clanek IF 2.474
  • M. Gagic, S. Kociova, K. Smerkova, H. Michalkova, M. Setka, P. Svec, J. Pribyl, J. Masilko, R. Balkova, Z. Heger, L. Richtera, V. Adam and V. Milosavljevic, One-pot synthesis of natural amine-modified biocompatible carbon quantum dots with antibacterial activity, J. Colloid Interface Sci. 580 (2020), pp. 30-48.
  • Sur VP, Mazumdar A, Ashrafi AM, Mukherjee S, Milosavljevic V, Michalkova H, Kopel P, Richtera L, Moulick A (2020) A novel biocompatible titanium–gadolinium quantum dot as a bacterial detecting agent with high antibacterial activity. Nanomaterials 10: 1-19. clanek IF 4.034
  • Sur VP, Mazumdar A, Kopel P, Mukherjee S, Vitek P, Michalkova H, Vaculovicova M, Moulick A (2020) A novel ruthenium based coordination compound against pathogenic bacteria Int J Mol Sci 21: 1-18. clanek IF 4.183
  • Milosavljevic V, Jamroz E, Gagic M, Haddad Y, Michalkova H, Balkova R, Tesarova B, Moulick A, Heger Z, Richtera L, Kopel P, Adam V (2020) Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery.’Biomacromolecules 21: 418-434. clanek IF 5.667
  • Gagic M, Nejdl L, Xhaxhiu K, Cernei N, Zitka O, Jamroz E, Svec P, Richtera L, Kopel P, Milosavljevic V, Adam V (2020) Fully automated process for histamine detection based on magnetic separation and fluorescence detection. Talanta 212: 1-10. clanek IF 4.916
  • Michalkova H, Skubalova Z, Sopha H, Strmiska V, Tesarova B, Dostalova S, Svec P, Hromadko L, Motola M, Macak JM, Adam V, Heger Z (2020) Complex cytotoxicity mechanism of bundles formed from self-organised 1-D anodic TiO2 nanotubes layers. J Hazard Mater 388: 1-12. clanek IF 7.650
  • Jamroz E, Kopel P, Tkaczewska J, Dordevic D, Jancikova S, Kulawik P, Milosavljevic V, Dolezelikova K, Smerkova K, Svec P, Adam V (2019) Nanocomposite furcellaran films – The influence of nanofillers on functional properties of furcellaran films and effect on linseed oil preservation. Polymers 11: 1-17. clanek IF 3.164
  • Durovic, A.; Stojanovic, Z.; Kravic, S.; Kos, J.; Richtera, L.; Electrochemical Determination of Vitamin D3 in Pharmaceutical Products by Using Boron Doped Diamond Electrode . Electroanalysis 2019, in press, . DOI: 10.1002/elan.201900532 .
  • Bytesnikova, Z.; Richtera, L.; Smerkova, K.; Adam, V.; The graphene oxide as a tool for antibiotic resistance genes removal: A review. Environ. Sci. Pollut. Res. 2019, 26, 20148-20163. DOI: 10.1007/s11356-019-05283-y.
  • Jamroz, E.; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Makarewicz, M.; Development of furcellaran-gelatin films with Se-AgNPs as an active packaging system for extension of mini kiwi shelf life. Food Packaging Shelf 2019, 21, 1-9. DOI: 10.1016/j.fpsl.2019.100339.
  • Jamroz, E.; Kulawik, P.; Kopel, P.; Balkova, R.; Hynek, D.; Bytesnikova, Z.; Gagic, M.; Milosavljevic, V.; Adam, V.; Intelligent and active composite films based on furcellaran: Structural characterization, antioxidant and antimicrobial activities. Food Packaging Shelf 2019, 22, 1-10. DOI: 10.1016/j.fpsl.2019.100405 .
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Gagic, M.; Jamroz, E.; Krizkova, S.; Milosavljevic, V.; Kopel, P.; Adam, V.; Current trends in detection of histamine in food and beverages . J. Agric. Food Chem. 2019, 67, 773-783. DOI: 10.1021/acs.jafc.8b05515.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.;Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Koudelkova, Z.; Bytesnikova, Z.; Xhaxhiu, K.; Kremplova, M.; Hynek, D.; Adam, V.; Richtera, L.; Electrochemical evaluation of selenium (IV) removal from its aqueous solutions by unmodified and modified graphene oxide. Molecules 2019, 24, 1-13. DOI: 10.3390/molecules24061063.
  • Ashrafi, A. M.; Richtera, L.; Preparation and characterization of carbon paste electrode bulk-modified with multiwalled carbon nanotubes and its application in a sensitive assay of antihyperlipidemic simvastatin in biological samples. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24122215.
  • Ashrafi, A. M.; Sys, M.; Sedlackova, E.; Farag, A. S.; Adam, V.; Pribyl, J.; Richtera, L.; Application of the enzymatic electrochemical biosensors for monitoring non-competitive inhibition of enzyme activity by heavy metals. Sensors 2019, 19, 1-15. DOI: 10.3390/s19132939.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Jamr; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Kucharek, M.; Makarewicz, M.; Adam, V.; Development and characterisation of furcellaran-gelatin films containing SeNPs and AgNPs that have antimicrobial activity. Food Hydrocolloids 2018, 83, 9-16. DOI: 10.1016/j.foodhyd.2018.04.028.
  • Jimenez Jimenez, A. M.; Moulick, A.; Richtera, L.; Krejcova, L.; Kalina, L.; Datta, R.; Svobodova, M.; Hynek, D.; Masarik, M.; Heger, Z.; Adam, V.; Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. Sens. Actuator B-Chem. 2018, 258, 295-303. DOI: 10.1016/j.snb.2017.11.074.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Moulick, A.; Heger, Z.; Milosavljevic, V.; Richtera, L.; Barroso; Merlos, M. A. R.; Buchtelova, H.; Podgajny, R.; Hynek, D.; Kopel, P.; Adam, V.; Real-time visualization of cell membrane damage using gadolinium Schiff base complex-doped quantum dots. ACS Appl. Mater. Interfaces 2018, 10, 35859-35868. DOI: 10.1021/acsami.8b15868.