Laboratory of nanomedicine

The research in the lab of nanomedicine supplies a comprehensive review of the application of bio-nanomaterials enabling to specifically and safely target selected tumour cells. A whole scale of low-molecular substances, peptides or proteins, serves for ensuring the interaction with concrete receptors of the malign cells. However, another line of research focuses also on in silico design and testing of new peptides biomimicking natural protein-protein interactions. Our access to the CESNET infrastructure and to a number of advanced software applications is very supportive for the above purpose. Special highlight deserves the biocompatibility of the utilized materials, and namely not only in vitro, but also in vivo, in applications using tumorized immune-deficient animal models. The biocompatibility is boosted by methods of molecular cloning and protein engineering. The interactions of nanomaterials with healthy tissue are subject to complex testing, applying advanced molecular biological and analytical techniques.

Laboratoř nanomedicíny

The highly interdisciplinary research of the lab is enabled by the composition of its team, consisting of experts in the field of biomedical engineering, in silico modelling, molecular and cell biology. The excellent standard of the nanomedical lab is further guaranteed by the most recent equipment for 2D and 3D cultivation of cell cultures, flow cytometer and a confocal fluorescence microscope.


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Ing. Simona Rex, Ph.D.

Head – Laboratory of nanomedicine
Academic staff – assistant


Phone: 420 545 133 274
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3016
Email:


Team members

Topics of Ph.D. theses

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Publications

  • Skubalova, Z.; Michalkova, H.; Michalek, P.; Strmiska, V.; Guran, R.; Merlos, M. A. R.; Castkova, K.; Hynek, D.; Pekarik, V.; Zitka, O.; Adam, V.; Heger, Z.; Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloid Surf. B-Biointerfaces 2019, 182, 1-10. DOI: 1.
  • Haddad, Y.; Adam, V.; Heger, Z.; Rotamer dynamics: analysis of rotamers in molecular dynamics simulations of proteins . Biophys. J. 2019, 116, 2062-2072. DOI: 10.1016/j.bpj.2019.04.017.
  • Tesarova, B.; Charousova, M.; Dostalova, S.; Bienko, A.; Kopel, P.; Kruszynski, R.; Hynek, D.; Michalek, P.; Eckschlager, T.; Stiborova, M.; Adam, V.; Heger, Z.; Folic acid-mediated re-shuttling of ferritin receptor specificity towards a selective delivery of highly cytotoxic nickel(II) coordination compounds. Int. J. Biol. Macromol. 2019, 126, 1099-1111. DOI: 10.1016/j.ijbiomac.2018.12.128.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Al Zoubi, M. S.; Al-Batayneh, K. M.; Al-Trad, B.; Haddad, Y.; Hussein, E.; Al Khateeb, W.; Aljabali, A. A. A.; Bodoor, K.; Shehab, M.; Al Hamad, M.; Muhaidat, R.; Evaluation of vitamin B12, folate and ferritin serum levels in Jordanian population. J. Nutr. Sci. Vitaminol. 2019, 65, 309-317. DOI: 10.3177/jnsv.65.309.
  • Indra, R.; Pompach, P.; Martinek, V.; Takacsova, P.; Vavrova, K.; Heger, Z.; Adam, V.; Eckschlager, T.; Kopeckova, K.; Arlt, V. M.; Stiborova, M.; Identification of human enzymes oxidizing the anti-thyroid-cancer drug vandetanib and explanation of the high efficiency of cytochrome P450 3A4 in its oxidation . Int. J. Mol. Sci. 2019, 20, 1-22. DOI: 10.3390/ijms20143392.
  • Alves, M. N.; Nesterenko, P. N.; Paull, B.; Haddad, P. R.; Macka, M.; Separation of superparamagnetic magnetite nanoparticles by capillary zone electrophoresis using non-complexing and complexing electrolyte anions and tetramethylammonium as dispersing additive. Electrophoresis 2018, 39, 1429-1436. DOI: 10.1002/elps.201800095.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Dostalova, S.; Polanska, H.; Svobodova, M.; Balvan, J.; Krystofova, O.; Haddad, Y.; Krizkova, S.; Masarik, M.; Eckschlager, T.; Stiborova, M.; Heger, Z.; Adam, V.; Prostate-specific membrane antigen-targeted site-directed antibody-conjugated apoferritin nanovehicle favorably influences in vivo side effects of doxorubicin . Sci. Rep. 2018, 8, 1-13. DOI: 10.1038/s41598-018-26772-z.
  • Buchtelova, H.; Strmiska, V.; Skubalova, Z.; Dostalova, S.; Michalek, P.; Krizkova, S.; Hynek, D.; Kalina, L.; Richtera, L.; Moulick, A.; Adam, V.; Heger, Z.; Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping. J. Nanobiotechnol. 2018, 16, 1-14. DOI: 10.1186/s12951-018-0369-7.
  • Jelinkova, P.; Splichal, Z.; Jimenez Jimenez, A. M.; Haddad, Y.; Mazumdar, A.; Sur, V. P.; Milosavljevic, V.; Kopel, P.; Buchtelova, H.; Guran, R.; Zitka, O.; Richtera, L.; Hegerova, D.; Heger, Z.; Moulick, A.; Adam, V.; Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus. Infect. Drug Resistance 2018, 2018, 1807. DOI: 10.2147/IDR.S160975.
  • Bodoor, K.; Melhem, D.; Shotar, A.; Alkhatib, A.; Batiha, O.; Abu-Sheikha, A.; Haddad, Y.; Prevalence of cervical human papillomavirus is associated with p16 expression and irregular menstrual cycles among married Jordanian women. European Journal of Gynaecological Oncology 2018, 39, 779-785. DOI: 10.12892/ejgo4318.2018.
  • Moulick, A.; Richtera, L.; Milosavljevic, V.; Cernei, N.; Haddad, Y.; Zitka, O.; Kopel, P.; Heger, Z.; Adam, V.; Advanced nanotechnologies in avian influenza: Current status and future trends – A review . Analytica Chimica Acta 2017, 983, 42-53 . DOI: 10.1016/j.aca.2017.06.045.
  • Bodoor, K.; Al-Ghabkari, A.; Matalka, I.; Haddad, Y.; Alkhateeb, A.; Jaradat, S.; Jaradat, Z.W.; Al-Ghazo, M.; Abu-Sheikha, A.; Abu Jalboush, S; Jarun, Y.; Assessment of p53 mutations, expression and prognosis in bladder cancer patients from Jordan: Identification of novel deletion mutations in the DNA-binding domain. Meta Gene 2017, 12, 33-42. DOI: 10.1016/j.mgene.2017.01.002.
  • Milosavljevic, V.; Jelinkova, P.; J; Moulick, A.; Haddad, Y.; Buchtelova, H.; Krizkova, S.; Heger, Z.; Kalina, L.; Richtera, L.; Kopel, P.; Adam, V.; Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms. Molecular Pharmaceutics 2017, 14, 221-233. DOI: 10.1021/acs.molpharmaceut.6b00807.
  • Anyz, J.; Vyslouzilova, L.; Vaculovic, T.; Tvrdonova, M.; Kanicky, V.; Haase, H.; Horak, V.; Stepankova, O.; Heger, Z.; Adam, V.; Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Sci. Rep. 2017, 7, 1-13. DOI: 10.1038/srep40169.
  • Haddad, Y.; Heger, Z.; Adam, V.; Targeting Neuroblastoma Cell Surface Proteins: Recommendations for Homology Modeling of hNET, ALK, and TrkB. Front. Molec. Neurosci. 2017, 10, 1-7. DOI: 10.3389/fnmol.2017.00007.