Research group for synthesis and chemical analysis

The foundations of electrochemistry were laid almost a hundred years ago. The invention of polarography brought the Czech scientist Jaroslav Heyrovský the award of the Nobel Prize. Thanks to this fact, but not only, electrochemistry can look back at a great tradition in the Czech Republic.

Our laboratory is well adapted for the electrochemical detection of nucleic acids (DNA, RNA, including the small RNA and single nucleotides), proteins and peptides containing thiol groups (metallo-thionein) and a number of elements including heavy metals or semi-metals (such as Cd, Pb, Cu, Zn, Cr, Ti, Hg, Ag, Fe, As, Te and Se). The detection of the investigated substances is carried on both by the classical methods of polarography and by those utilizing solid or pasty electrodes.


Výzkumná skupina syntézy a chemické analýzy

The conjugation with electroactive markers (such as the process on the basis of quantum dots) can be used for substantially increasing the response of the analyte.

Among further domains of our interest also the development of sensors and biosensors should be mentioned (detection of platinum cytostatics, the study of DNA methylation in correlation with the origin of tumour diseases, determining the contamination of the environment by heavy metals etc.).

Invitation to contribute to the Special Issue of Processes “Nanocarbon Materials for Virus Reduction and Detection (Impact Factor: 2.753)


RNDr. Lukáš Richtera, Ph.D.

Head – Research group for synthesis and chemical analysis
Assistant Professor

Phone: 420 545 133 311
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N2016
Researcher ID: N-9991-2014
ORCID: 0000-0002-8288-3999
Scopus ID: 12040049600

Team members

Topics of Ph.D. theses

  • Synthesis of potential antimicrobial peptides and study of bacterial resistance mechanisms and enzymatic activity
  • Development of microfluidic Lab-on-a-chip exploiting magnetic nanoparticles for biogenic amines
  • Label-free electrochemical aptasensor for detection of cancer biomarker
  • Synthesis of potential antimicrobial peptides and study of bacterial resistance mechanisms and enzymatic activity
  • Development of microfluidic Lab-on-a-chip exploiting magnetic nanoparticles for biogenic amines
  • Electrochemical detection of bacteria using the nanomaterials
  • Nanomaterials for plant protection
  • Nanomaterials for application in agricultural practice
  • Carbon nanomaterials and their composites for bioapplications
  • Optional topic




  • ASHRAFI, A.; BYTESNIKOVA, Z.; BAREK, J.; RICHTERA, L.; ADAM, V. A Critical Comparison of Natural Enzymes and Nanozymes in Biosensing and Bioassays. Biosens. Bioelectron., 2021, vol. 192. p. 1-18. ISSN 0956-5663. IF 10.618
  • ASSERGHINE, A.; ASHRAFI, A.; MUKHERJEE, A.; PETRLAK, F.; HEGER, Z.; SVEC, P.; RICHTERA, L.; NAGY, L.; SOUTO, R. M.; NAGY, G.; ADAM, V. In situ investigation of the cytotoxic and interfacial characteristics of titanium when galvanically coupled with magnesium using scanning electrochemical microscopy. ACS Appl. Mater. Interfaces, 2021, vol. 13. p. 43587-43596. ISSN 1944-8244. IF 9.229
  • JANOVA, A.; KOLACKOVA, I.; BYTESNIKOVA, Z.; CAPAL, P.; CHALOUPSKY, P.; SVEC, P.; RIDOSKOVA, A.; CERNEI, N.; KLEJDUS, B.; RICHTERA, L.; ADAM, V.; HUSKA, D. New insights into mechanisms of copper nanoparticle toxicity in freshwater algae Chlamydomonas reinhardtii: Effects on the pathways of secondary metabolites. Algal Res., 2021, vol., p. 1-11. ISSN 2211-9264. IF 4.401
  • BENES, J.; TODOROVSKA, V.; BUDILOVA, K.; KOVARNIK, J.; PAVELKA, J.; ATANASOSKA, N.; BUMEL, J.; FLORENZANO, A.; MAJEROVICOVA, T.; VONDROVSKY, V.; PTAKOVA, M.; BEDNAR, P.; RICHTERA, L.; KUCERA, L. What about dinner? Chemical and microresidue analysis reveals the function of late neolithic ceramic pans. Molecules, 2021, vol. 26. p. 1-16. ISSN 1420-3049. IF 4.411
  • BYTESNIKOVA, Z.; ADAM, V.; RICHTERA, L. Graphene oxide as a novel tool for mycotoxin removal. Food Control, 2021, vol. 121. p. 1-9. ISSN 0956-7135. IF 5.548
  • KUCERA, L.; ROZSYPAL, J.; BEDNAR, P.; BREZINA, M.; KALINA, L.; BEZDICKA, P.; MASLAN, M.; RICHTERA, L. “Gold corrosion”: An alternative source of red stains on gold coins. Materialia, 2021, vol. 15. p. 1-8. ISSN 2589-1529. IF 0.000
  • MITREVSKA, K.; MILOSAVLJEVIC, V.; GAGIC, M.; RICHTERA, L.; ADAM, V. 2D transition metal dichalcogenide nanomaterial-based miRNA biosensors. Appl. Mater. Today, 2021, vol. 23. p. 1-23. ISSN 2352-9407. IF 10.041
  • MUKHERJEE, S.; BOUTANT, E.; REAL, E.; MELY, Y.; ANTON, H. Imaging viral infection by fluorescence microscopy: Focus on HIV-1 early stage. Viruses-Basel, 2021, vol. 13. p. 1-21. ISSN 1999-4915. IF 5.048
  • NADTOCHAEVA, P.; BADINOVA, E.; RICHTERA, L.; VOBĚRKOVÁ, S. Crude laccase-based biosensor for the detection of catechin. Biotechnology & Biotechnological Equipment, 2021, vol. 35. ISSN 1310-2818. IF 1.452
  • PECENKA, J.; BYTESNIKOVA, Z.; KISS, M.; PENAZOVA, E.; BARANEK, M.; EICHMEIER, A.; TEKIELSKA, D.; RICHTERA, L.; POKLUDA, R.; ADAM, V. Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment. Mater. Today Commun., 2021, vol. 27. p. 1-8. ISSN 2352-4928. IF 3.383
  • STEPANKOVA, H.; SWIATKOWSKI, M.; KRUSZYNSKI, R., .; SVEC, P.; MICHALKOVA, H.; SMOLIKOVA, V.; RIDOSKOVA, A.; SPLICHAL, Z.; MICHALEK, P.; RICHTERA, L.; KOPEL, P.; ADAM, V.; HEGER, Z.; REX, S. The anti-proliferative activity of coordination compound-based ZnO nanoparticles as a promising agent against triple negative breast cancer cells. Int. J. Nanomed., 2021, vol. 2021. p. 4431-4449. ISSN 1178-2013. IF 6.400
  • SYS, M.; MUKHERJEE, A.; JASHARI, G.; ADAM, V.; ASHRAFI, A.; NOVAK, M.; RICHTERA, L. Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator? Materials, 2021, vol. 14. p. 1-18. ISSN 1996-1944. IF 3.623
  • VANOVA, V.; MITREVSKA, K.; MILOSAVLJEVIC, V.; HYNEK, D.; RICHTERA, L.; ADAM, V. Peptide-based electrochemical biosensors utilized for protein detection. Biosens. Bioelectron., 2021, vol. 180. p. 1-19. ISSN 0956-5663. IF 10.618
  • Durovic, A.; Stojanovic, Z.; Kravic, S.; Kos, J.; Richtera, L.; Electrochemical Determination of Vitamin D3 in Pharmaceutical Products by Using Boron Doped Diamond Electrode . Electroanalysis 2019, in press, . DOI: 10.1002/elan.201900532 .
  • Bytesnikova, Z.; Richtera, L.; Smerkova, K.; Adam, V.; The graphene oxide as a tool for antibiotic resistance genes removal: A review. Environ. Sci. Pollut. Res. 2019, 26, 20148-20163. DOI: 10.1007/s11356-019-05283-y.
  • Jamroz, E.; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Makarewicz, M.; Development of furcellaran-gelatin films with Se-AgNPs as an active packaging system for extension of mini kiwi shelf life. Food Packaging Shelf 2019, 21, 1-9. DOI: 10.1016/j.fpsl.2019.100339.
  • Jamroz, E.; Kulawik, P.; Kopel, P.; Balkova, R.; Hynek, D.; Bytesnikova, Z.; Gagic, M.; Milosavljevic, V.; Adam, V.; Intelligent and active composite films based on furcellaran: Structural characterization, antioxidant and antimicrobial activities. Food Packaging Shelf 2019, 22, 1-10. DOI: 10.1016/j.fpsl.2019.100405 .
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Petrovic, S.; Guzsvany, V.; Rankovic, N.; Beljin, J.; Roncevic, S.; Dalmacija, B.; Ashrafi, A. M.; Konya, Z.; Svancara, I.; Vytras, K.; Trace level voltammetric determination of Zn(II) in selected nutrition related samples by bismuth-oxychloride-multiwalled carbon nanotube composite based electrode. Microchemical Journal 2019, 146, 178-186. DOI: 10.1016/j.microc.2018.12.053.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Gagic, M.; Jamroz, E.; Krizkova, S.; Milosavljevic, V.; Kopel, P.; Adam, V.; Current trends in detection of histamine in food and beverages . J. Agric. Food Chem. 2019, 67, 773-783. DOI: 10.1021/acs.jafc.8b05515.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.; Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Koudelkova, Z.; Bytesnikova, Z.; Xhaxhiu, K.; Kremplova, M.; Hynek, D.; Adam, V.; Richtera, L.; Electrochemical evaluation of selenium (IV) removal from its aqueous solutions by unmodified and modified graphene oxide. Molecules 2019, 24, 1-13. DOI: 10.3390/molecules24061063.
  • Ashrafi, A. M.; Richtera, L.; Preparation and characterization of carbon paste electrode bulk-modified with multiwalled carbon nanotubes and its application in a sensitive assay of antihyperlipidemic simvastatin in biological samples. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24122215.
  • Ashrafi, A. M.; Sys, M.; Sedlackova, E.; Farag, A. S.; Adam, V.; Pribyl, J.; Richtera, L.; Application of the enzymatic electrochemical biosensors for monitoring non-competitive inhibition of enzyme activity by heavy metals. Sensors 2019, 19, 1-15. DOI: 10.3390/s19132939.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Jamr; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Kucharek, M.; Makarewicz, M.; Adam, V.; Development and characterisation of furcellaran-gelatin films containing SeNPs and AgNPs that have antimicrobial activity. Food Hydrocolloids 2018, 83, 9-16. DOI: 10.1016/j.foodhyd.2018.04.028.
  • Merkel, ; Ashrafi, A; E; Bipolar membrane electrodialysis assisted pH correction of milk whey. J. Membr. Sci. 2018, 555, 185-196. DOI: 10.1016/j.memsci.2018.03.035.