Laboratory of sensing techniques

Nowadays, analytical techniques do not benefit only from expensive benchtop devices which are suitable for laboratory use only. Technological progress enables to integrate several simple elements such as miniaturized electrodes, LED diodes, computers, and other necessary hardware platforms into simple and cheap bioanalytical devices. The key tools in this field are 3D printing and specially designed micro and nanoscale particles.

The mission of laboratory is design and fabrication of benchtop and/or portable device which can be controlled by Bluetooth of cell phones. All the platforms are built to be fully operated and tested in laboratory but as another evolution step some of them could be applicable on the site of use meets the requirements for Point of Care Testing (POCT).


Photo

doc. RNDr. Ondřej Zítka, Ph.D.

Head – Research group for biomarkers
Academic staff – Associate Professor


Phone: +420 545 133 285
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N2004
Email:
Researcher ID: E-1107-2012
ORCID: 0000-0001-7607-5058
Scopus ID: 14012648400


Team members

  • Ing. Jiří Kudr, Ph.D.
  • Mgr. Jiří Pospíšil
  • Ing. Jan Zítka
  • Bc. Michal Pláteník

Topics of Ph.D. theses

  • Průtokové systémy s elektrochemickou detekcí pro analýzu biomolekul
  • Prostorové mapování proteinů v živočišné tkáni s využitím MALDI MSI
  • Elektrochemické biosenzory využívajících nanotechnologie pro analýzu biomolekul

Publications

  • Strmiska, V.; Michalek, P.; Lackova, Z.; Guran, R.; Krizkova, S.; Vanickova, L.; Zitka, O.; Stiborova, M.; Eckschlager, T.; Klejdus, B.; Pacik, D.; Tvrdikova, E.; Keil, C.; Haase, H.; Adam, V.; Heger, Z.; Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis. Mol. Oncol. 2019, 13, 1002-1017. DOI: 10.1002/1878-0261.12439.
  • Skubalova, Z.; Michalkova, H.; Michalek, P.; Strmiska, V.; Guran, R.; Merlos, M. A. R.; Castkova, K.; Hynek, D.; Pekarik, V.; Zitka, O.; Adam, V.; Heger, Z.; Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloid Surf. B-Biointerfaces 2019, 182, 1-10. DOI: 1.
  • Vanickova, L.; Guran, R.; Kollar, S.; Emri, G.; Krizkova, S.; Do, T.; Heger, Z.; Zitka, O.; Adam, V.; Mass spectrometric imaging of cysteine rich proteins in human skin. Int. J. Biol. Macromol. 2019, 125, 270-277. DOI: 10.1016/j.ijbiomac.2018.11.272.
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Vsiansky; Svobodova; Gumulec; Cernei, N.; Sterbova, D.; Zitka, O.; Kostrica, R.; Smilek; Plzak; Betka; Kalfert; Masarik, M.; Raudenska, M.; Prognostic significance of serum free amino acids in head and neck cancers. Cells 2019, 8, 1-12. DOI: 10.3390/cells8050428.
  • Kudr, J.; Adam, V.; Zitka, O.; Fabrication of graphene/molybdenum disulfide composites and their usage as actuators for electrochemical sensors and biosensors. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24183374.
  • Sur, V. P.; Kominkova, M.; Buchtova, Z.; Dolezelikova, K.; Zitka, O.; Moulick, A.; CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing. Nanomaterials 2019, 9, 1-15. DOI: 10.3390/nano9101463.
  • Gumulec, J.; Raudenska, M.; Pacik, D.; Plevova, M.; Sorokac-Kubolkova, A.; Lackova, Z.; Cernei, N.; Strmiska, V.; Zitka, O.; Heger, Z.; Adam, V.; Post- treatment urinary sarcosine as a predictor of recurrent relapses in patients with prostate cancer. Cancer Med. 2018, 7, 5411-5419. DOI: 10.1002/cam4.1767.
  • Zitka, O.; Kudr, J.; Labuda, J.; Xhaxhiu, K.; Adam, V.; Separation of Nucleobases Using High-Performance Liquid Chromatography Coupled with Voltammetric Scanning. Electroanalysis 2018, 30, 1857-1861. DOI: 10.1002/elan.201800175.
  • Vaneckova, T.; Smerkova, K.; Zitka, J.; Hynek, D.; Zitka, O.; Hlavacek, A.; Foret, F.; Adam, V.; Vaculovicova, M.; Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis. Electrophoresis 2018, 39, 2246-2252. DOI: 10.1002/elps.201700483.
  • Zdrazil, L.; Zahradnicek, R.; Mohan, R.; Sedlacek, P.; Nejdl, L.; Schmiedova, V.; Pospisil, J.; Horak, M.; Weiter, M.; Zmeskal, O.; Hubalek, J.; Preparation of graphene quantum dots through liquid phase exfoliation method. J. Lumines. 2018, 204, 203-208. DOI: 10.1016/j.jlumin.2018.08.017.
  • Kudr, J.; Klejdus, B.; Adam, V.; Zitka, O.; Magnetic solids in electrochemical analysis. TRAC-Trends Anal. Chem. 2018, 98, 104-113. DOI: 10.1016/j.trac.2017.10.023.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Jelinkova, P.; Splichal, Z.; Jimenez Jimenez, A. M.; Haddad, Y.; Mazumdar, A.; Sur, V. P.; Milosavljevic, V.; Kopel, P.; Buchtelova, H.; Guran, R.; Zitka, O.; Richtera, L.; Hegerova, D.; Heger, Z.; Moulick, A.; Adam, V.; Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus. Infect. Drug Resistance 2018, 2018, 1807. DOI: 10.2147/IDR.S160975.
  • Buchtova, Z.; Lackova, Z.; Kudr, J.; Zitka, Z.; Skoda, J.; Zitka, O.; Capillary blood GSH level monitoring using electrochemical method adapted for micro volumes. Molecules 2018, 23, 1-10. DOI: 10.3390/molecules23102504.
  • Duskova, K.; Vesely, S.; Silva, J. D.; Cernei, N.; Zitka, O.; Heger, Z.; Adam, V.; Havlova, K.; Babjuk, M.; Differences in Urinary Amino Acid Patterns in Individuals with Different Types of Urological Tumor Urinary Amino Acid Patterns as Markers of Urological Tumors. In Vivo 2018, 32, 425-429. DOI: 10.21873/invivo.11257.
  • Raudenska, M.; Dvorakova, V.; Pacal, L.; Chalasova, K.; Kratochvilova, M.; Gumulec, J.; Ruttkay-Nedecky, B.; Zitka, O.; Kankova, K.; Adam, V.; Masarik, M.; Levels of heavy metals and their binding protein metallothionein in type 2 diabetics with kidney disease. J. Biochem. Mol. Toxicol. 2017, 31, 1-5. DOI: 10.1002/jbt.21891.
  • Nejdl, L.; Zitka, J.; Mravec, F.; Milosavljevic, V.; Zitka, O.; Kopel, P.; Adam, V.; Vaculovicova, M.; Real-time monitoring of the UV-induced formation of quantum dots on a milliliter, microliter, and nanoliter scale. Microchim. Acta 2017, 184, 1489-1497. DOI: 10.1007/s00604-017-2149-8.
  • Moulick, A.; Richtera, L.; Milosavljevic, V.; Cernei, N.; Haddad, Y.; Zitka, O.; Kopel, P.; Heger, Z.; Adam, V.; Advanced nanotechnologies in avian influenza: Current status and future trends – A review . Analytica Chimica Acta 2017, 983, 42-53 . DOI: 10.1016/j.aca.2017.06.045.
  • Kudr, J.; Richtera, L.; Xhaxhiu, K.; Hynek, D.; Heger, Z.; Zitka, O.; Adam, V.; Carbon dots based FRET for the detection of DNA damage. Biosens. Bioelectron. 2017, 92, 133-139. DOI: 10.1016/j.bios.2017.01.067.