Research Group for Instrumental Analysis and Bioanalysis

A multidisciplinary research group is focused on a wide range of scientific topics covering plant biology, elemental analysis and bioanalysis. Newly developed or optimized methods are relevant not only to the field of conventional applications (environmental analysis, food analysis, medical diagnostics, etc.), but are capable to assess important biological context or to monitor external factors for key biological processes.

The use of sophisticated analytical instruments (spectrometric or separation techniques) as well as cutting-edge approaches of modern molecular biology (genome editing) creates a complex research strategy.

One of the possible advantages of multidisciplinarity and multi-instrumentality is the ability to obtain information not only about metal content (AAS, (LA)-ICP-MS), but also about key biomolecules (DART / DESI-MS, GC-MS) including their spatial distribution.

The research group has a very good network for collaboration with international co-workers, but also for commercial activities and especially in the field of metals and their speciation.



Vaculovicova marketa

doc. Mgr. Markéta Vaculovičová, Ph.D.

Head – Research Group for Instrumental Analysis and Bioanalysis
Academic staff – Associate Professor

Phone: 420545 13 32 90
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3010
Researcher ID: E-5583-2016
ORCID: 0000-0002-6771-1304

Team members



Topics of Ph.D. theses

  • Optional topic
  • Free theme-metals in the environment
  • Bioavailability of mercury for crops
  • Paperfluidic analytical devices for applications in diagnostics
  • Optical detection techniques for bioanalysis
  • Development of novel approaches for clinical diagnostics
  • Design and development of biosensors for rapid and use-friendly diagnostics


  • AZV ČR: Role signalizace monocytárních buněk, jejich metabolických změn a transkripčních faktorů u pacientů se závažnou sepsí.. 2018-2021.
  • FAO/IAEA: Kutikulární uhlovodíky: Nový chemotaxonomický a chemoekologický
    nástroj pro kontrolu zemědělských škůdců vrtulí z rodů Zeugodacus a Bactrocera. 2019-2023.
  • GAČR: Paperfluidická přenosná zařizení pro rychlou a nízkonákladovou analýzu bez instrumentální detekce. 2019-2021.


  • KHUNKITCHAI, N.,NUCHTAVORN, N.,RYPAR, T.,VLCNOVSKA, M.,NEJDL, L.,VACULOVICOVA, M.,MACKA, M. Uv-light-actuated in-situ preparation of paper@zncd quantum dots for paper-based enzymatic nanoreactors. Chemical Engineering Journal. 2022, vol. 428. no., pp. 132508.
  • NUCHTAVORN, N.,RYPAR, T.,NEJDL, L.,VACULOVICOVA, M.,MACKA, M. Distance-based detection in analytical flow devices: From gas detection tubes to microfluidic chips and microfluidic paper-based analytical devices TrAC – Trends in Analytical Chemistry. 2022, in press.
  • VANÍČKOVÁ, L.,DO, T.,VEJVODOVÁ, M.,HORÁK, V.,HUBÁLEK, M.,EMRI, G.,ZEMÁNKOVÁ, K.,PAVELICOVÁ, K.,KŘÍŽKOVÁ, S.,FALTUSOVÁ, V.,POMPEIANO, A.,VACULOVIČOVÁ, M.,ZÍTKA, O.,VACULOVIČ, T.,ADAM, V. Mapping of melim melanoma combining icp-ms and maldi-msi methods. International Journal of Biological Macromolecules. 2022, vol. 203. no., pp. 583-592.
  • VODOVA, M.,NEJDL, L.,PAVELICOVA, K.,ZEMANKOVA, K.,RYPAR, T.,SKOPALOVA STERBOVA, D.,BEZDEKOVA, J.,NUCHTAVORN, N.,MACKA, M.,ADAM, V.,VACULOVICOVA, M. Detection of pesticides in food products using paper-based devices by uv-induced fluorescence spectroscopy combined with molecularly imprinted polymers. Food Chemistry. 2022, vol. 380. no., pp. 132141.
  • ZEMANKOVA, K.,NEJDL, L.,BEZDEKOVA, J.,VODOVA, M.,PETERA, L.,PASTOREK, A.,CIVIS, S.,KUBELIK, P.,FERUS, M.,ADAM, V.,VACULOVICOVA, M. Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry. Microchemical Journal. 2021, vol. 167. no., pp. 7.
  • NEJDL, L.,HAVLIKOVA, M.,MRAVEC, F.,VACULOVIC, T.,FALTUSOVA, V.,PAVELICOVA, K.,BARON, M.,KUMSTA, M.,ONDROUSEK, V.,ADAM, V.,VACULOVICOVA, M. Uv-induced fingerprint spectroscopy. Food Chemistry. 2022, vol. 368. no., pp. 130499.
  • PAVELICOVA, K.,VANICKOVA, L.,HADDAD, Y.,NEJDL, L.,ZITKA, J.,KOCIOVA, S.,MRAVEC, F.,VACULOVIC, T.,MACKA, M.,VACULOVICOVA, M.,ADAM, V. Metallothionein dimerization evidenced by qd-based förster resonance energy transfer and capillary electrophoresis. International Journal of Biological Macromolecules. 2021, vol. 170. no., pp. 53-60.
  • Stepankova, H. et al. The Anti-Proliferative Activity of Coordination Compound-Based ZnO Nanoparticles as a Promising Agent Against Triple Negative Breast Cancer Cells. Int. J. Nanomed. in press (2021).
  • Rypar, T., Adam, V., Vaculovicova, M. & Macka, M. Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers. Sens. Actuator B-Chem. 341, 1-10, doi: (2021).
  • Pavelicova, K. et al. Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis. Int. J. Biol. Macromol. 170, 53-60 (2021).
  • Nejdl, L. et al. UV-Induced Fingerprint Spectroscopy. Food Chem. in press (2021).
  • Vaneckova, T., Bezdekova, A., Han, G., Adam, V. & Vaculovicova, M. Application of molecularly imprinted polymers as artificial receptors for imaging. Acta Biomater. 101, 444-458, doi:10.1016/j.actbio.2019.11.007 (2020).
  • Tesarova, B. et al. Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine. Appl. Mater. Today 18, 1-11, doi: (2020).
  • Smolikova, V., Pelcova, P., Ridoskova, A., Hedbavny, J. & Grmela, J. Development and evaluation of the iron oxide-hydroxide based resin gel for the diffusive gradient in thin films technique. Anal. Chim. Acta 1102, 36-45, doi: (2020).
  • Pelcova, P., Zouharova, I., Ridoskova, A. & Smolikova, V. Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model. Chemosphere 234, 373-378, doi:10.1016/j.chemosphere.2019.06.076 (2019).
  • Pastorek, A. et al. Prebiotic synthesis at impact craters: the role of Fe-clays and iron meteorites. Chem. Commun. 55, 10563-10566, doi:10.1039/c9cc04627e (2019).
  • Huang, L. et al. Designing next generation of photon upconversion: Recent advances in organic triplet-triplet annihilation upconversion nanoparticles. Biomaterials 201, 77-86, doi:10.1016/j.biomaterials.2019.02.008 (2019).
  • Stanisavljevic, M., Krizkova, S., Vaculovicova, M., Kizek, R. & Adam, V. Quantum dots-fluorescence resonance energy transfer-based nano-sensors and their application. Biosens. Bioelectron. 74, 562-574, doi:10.1016/j.bios.2015.06.076 (2015).
  • Nuchtavorn, N., Leanpolchareanchai, J., Suntornsuk, L. & Macka, M. Paper-based sol-gel thin films immobilized cytochrome P450 for enzyme activity measurement. Anal. Chim. Acta 1098, 86-93, doi: (2020).
  • Aydofan, C. et al. Miniaturized LC in Molecular Omics. Anal. Chem. 92, 11485-11497, doi: (2020).
  • Rahbar, M., Wheeler, A. R., Paull, B. & Macka, M. Ion-exchange based immobilization of chromogenic reagents on microfluidic paper analytical devices. Anal. Chem. 91, 8756-8761, doi:10.1021/acs.analchem.9b01288 (2019).
  • Alves, M. N., Miro, M., Breadmore, M. C. & Macka, M. Trends in analytical separations of magnetic (nano)particles. TRAC-Trends Anal. Chem. 114, 89-97, doi:10.1016/j.trac.2019.02.026 (2019).
  • Scolari, F., Valerio, F., Benelli, G., Papadopoulos, N. T. & Vanickova, L. Tephritid fruit fly semiochemicals: current knowledge and future perspectives Insects 12, 1-57, doi: (2021).
  • Vanickova, L., Pompeiano, A., Madera, P., Massad, T. J. & Vahalik, P. Terpenoid profiles of resin in the genus Dracaena are species specific. Phytochemistry 170, 1-8, doi:10.1016/j.phytochem.2019.112197 (2020).
  • Vahalik, P. et al. The conservation status and population mapping of the endangered Dracaena serrulata in the Dhofar mountains, Oman. Forests 11, 1-16, doi:10.3390/f11030322 (2020).
  • Vahalik, P. et al. Metrics of growth habit derived from the 3D tree point cloud used for species determination-A new approach in botanical taxonomy tested on Dragon tree group example. Forests 11, 1-14, doi:10.3390/f11030272 (2020).
  • Madera, P. et al. What we know and what we do not know about Dragon trees? Forests 11, 1-34, doi:10.3390/f11020236 (2020).
  • Nsanganwimana, F. et al. Potentials of Miscanthus x giganteus for phytostabilization of trace element-contaminated soils: Ex situ experiment. Ecotox. Environ. Safe. 214, 1-10, doi: (2021).