Research group for biomarkers

The resear group is focused on searching of novel biomarkers and developement of novel sensors and biosensors using nanomaterials employing optical and electrochemical methods. Acquired knowledges are than, publiched, transferred to the market, or applied durich service research. This al lis avalid in area of bioanalysis, area of designing of prototypes of bioanalytical devices or novel approaches.

Výzkumná skupina biomarkerů


doc. RNDr. Ondřej Zítka, Ph.D.

Head – Research group for biomarkers
Academic staff – Associate Professor

Phone: +420 545 133 285
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N2004
Researcher ID: E-1107-2012
ORCID: 0000-0001-7607-5058
Scopus ID: 14012648400

Team members

Topics of Ph.D. theses

  • Flow systems with electrochemical detection for the analysis of biomolecules
  • Spatial mapping of proteins in animal tissue using MALDI MSI
  • Electrochemical biosensors using nanotechnology for the analysis of biomolecules


  • TAČR TREND: Research and development of innovative solutions in the laboratory diagnostics (FW01010202)
  • TAČR TREND: Development of new laboratory tests for the diagnosis of inflammation, sepsis and cardiovasculardisease based on chemiluminescence analysis on automated platforms  (FW01010052)
  • GAČR: Portable Point-of-Care Platform Based on Lock-in Amplifier
  • MVČR: An ultrafast portable system to detect SARS-CoV-2. 01/2021 – 12/2022


  • PERIS-DIAZ, M. D.; GURAN, R.; DOMENE, C.; DE LOS RIOS, V.; ZITKA, O.; ADAM, V.; KREZEL, A. An Integrated Mass Spectrometry and Molecular Dynamics Simulations Approach Reveals the Spatial Organization Impact of Metal-Binding Sites on the Stability of Metal-Depleted Metallothionein-2 Species. J Am Chem Soc., 2021, vol. 143 p. 16486-16501. ISSN 1520-5126. IF 15.419
  • BEZDEKOVA, A.; VODOVA, M.; DOLEZELIKOVA, K.; ZITKA, J.; SMERKOVA, K.; ZITKA, O.; ADAM, V.; VACULOVICOVA, M. Detection of microbial contamination based on uracil-selective synthetic receptors. Talanta, 2021, vol. 224. p. 1-7. ISSN 0039-9140. IF 6.057
  • ZHANG, H.; PAJER, P.; KUDR, J.; ZITKA, O.; NEUZIL, P. Design considerations for point-of-need devices based on nucleic acid amplification for COVID-19 diagnostics and beyond. Biotechniques, 2021, vol. 71. p. 1-5. ISSN 0736-6205. clanek IF 1.993
  • KUDR, J.; MICHALEK, P.; ADAM, V.; ZITKA, O. COVID-19: A challenge for electrochemical biosensors. TRAC-Trends Anal. Chem., 2021, vol. 136. p. 1-9. ISSN 0165-9936. IF 12.296
  • PERIS-DIAZ, M. D.; GURAN, R.; ZITKA, O.; ADAM, V.; KREZEL, A. Mass spectrometry-based structural analysis of cysteine-rich metal-binding sites in proteins with MetaOdysseus R software. J. Proteome Res., 2021, vol. 20. p. 776-785. ISSN 1535-3893. IF 4.466
  • Strmiska, V.; Michalek, P.; Lackova, Z.; Guran, R.; Krizkova, S.; Vanickova, L.; Zitka, O.; Stiborova, M.; Eckschlager, T.; Klejdus, B.; Pacik, D.; Tvrdikova, E.; Keil, C.; Haase, H.; Adam, V.; Heger, Z.; Sarcosine is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-Dnmts axis. Mol. Oncol. 2019, 13, 1002-1017. DOI: 10.1002/1878-0261.12439.
  • Skubalova, Z.; Michalkova, H.; Michalek, P.; Strmiska, V.; Guran, R.; Merlos, M. A. R.; Castkova, K.; Hynek, D.; Pekarik, V.; Zitka, O.; Adam, V.; Heger, Z.; Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloid Surf. B-Biointerfaces 2019, 182, 1-10. DOI: 1.
  • Vanickova, L.; Guran, R.; Kollar, S.; Emri, G.; Krizkova, S.; Do, T.; Heger, Z.; Zitka, O.; Adam, V.; Mass spectrometric imaging of cysteine rich proteins in human skin. Int. J. Biol. Macromol. 2019, 125, 270-277. DOI: 10.1016/j.ijbiomac.2018.11.272.
  • Bacova, R.; Klejdus, B.; Ryant, P.; Cernei, N.; Adam, V.; Huska, D.; The effects of 5-azacytidine and cadmium on global 5-methylcytosine content and secondary metabolites in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus quadricauda. J. Phycol. 2019, 55, 329-342. DOI: 10.1111/jpy.12819.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.; Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Vsiansky; Svobodova; Gumulec; Cernei, N.; Sterbova, D.; Zitka, O.; Kostrica, R.; Smilek; Plzak; Betka; Kalfert; Masarik, M.; Raudenska, M.; Prognostic significance of serum free amino acids in head and neck cancers. Cells 2019, 8, 1-12. DOI: 10.3390/cells8050428.
  • Kudr, J.; Adam, V.; Zitka, O.; Fabrication of graphene/molybdenum disulfide composites and their usage as actuators for electrochemical sensors and biosensors. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24183374.
  • Sur, V. P.; Kominkova, M.; Buchtova, Z.; Dolezelikova, K.; Zitka, O.; Moulick, A.; CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing. Nanomaterials 2019, 9, 1-15. DOI: 10.3390/nano9101463.
  • Gumulec, J.; Raudenska, M.; Pacik, D.; Plevova, M.; Sorokac-Kubolkova, A.; Lackova, Z.; Cernei, N.; Strmiska, V.; Zitka, O.; Heger, Z.; Adam, V.; Post- treatment urinary sarcosine as a predictor of recurrent relapses in patients with prostate cancer. Cancer Med. 2018, 7, 5411-5419. DOI: 10.1002/cam4.1767.
  • Zitka, O.; Kudr, J.; Labuda, J.; Xhaxhiu, K.; Adam, V.; Separation of Nucleobases Using High-Performance Liquid Chromatography Coupled with Voltammetric Scanning. Electroanalysis 2018, 30, 1857-1861. DOI: 10.1002/elan.201800175.
  • Vaneckova, T.; Smerkova, K.; Zitka, J.; Hynek, D.; Zitka, O.; Hlavacek, A.; Foret, F.; Adam, V.; Vaculovicova, M.; Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis. Electrophoresis 2018, 39, 2246-2252. DOI: 10.1002/elps.201700483.
  • Zdrazil, L.; Zahradnicek, R.; Mohan, R.; Sedlacek, P.; Nejdl, L.; Schmiedova, V.; Pospisil, J.; Horak, M.; Weiter, M.; Zmeskal, O.; Hubalek, J.; Preparation of graphene quantum dots through liquid phase exfoliation method. J. Lumines. 2018, 204, 203-208. DOI: 10.1016/j.jlumin.2018.08.017.
  • Kudr, J.; Klejdus, B.; Adam, V.; Zitka, O.; Magnetic solids in electrochemical analysis. TRAC-Trends Anal. Chem. 2018, 98, 104-113. DOI: 10.1016/j.trac.2017.10.023.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Pacik, D.; Plevova, M.; Urbanova, L.; Lackova, Z.; Strmiska, V.; Necas, A.; Heger, Z.; Adam, V.; Identification of sarcosine as a target molecule for the canine olfactory detection of prostate carcinoma. Sci. Rep. 2018, 8, 1-5. DOI: 10.1038/s41598-018-23072-4.
  • Jelinkova, P.; Splichal, Z.; Jimenez Jimenez, A. M.; Haddad, Y.; Mazumdar, A.; Sur, V. P.; Milosavljevic, V.; Kopel, P.; Buchtelova, H.; Guran, R.; Zitka, O.; Richtera, L.; Hegerova, D.; Heger, Z.; Moulick, A.; Adam, V.; Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus. Infect. Drug Resistance 2018, 2018, 1807. DOI: 10.2147/IDR.S160975.
  • Buchtova, Z.; Lackova, Z.; Kudr, J.; Zitka, Z.; Skoda, J.; Zitka, O.; Capillary blood GSH level monitoring using electrochemical method adapted for micro volumes. Molecules 2018, 23, 1-10. DOI: 10.3390/molecules23102504.
  • Urbanova, L.; Horky, P.; Skladanka, J.; Pribilova, M.; Smolikova, V.; Nevrkla, P.; Cernei, N.; Lackova, Z.; Hedbavny, J.; Ridoskova, A.; Adam, V.; Kopel, P.; Antioxidant status of rats’ blood and liver affected by sodium selenite and selenium nanoparticles. PeerJ 2018, 6, 1-11. DOI: 10.7717/peerj.4862.
  • Duskova, K.; Vesely, S.; Silva, J. D.; Cernei, N.; Zitka, O.; Heger, Z.; Adam, V.; Havlova, K.; Babjuk, M.; Differences in Urinary Amino Acid Patterns in Individuals with Different Types of Urological Tumor Urinary Amino Acid Patterns as Markers of Urological Tumors. In Vivo 2018, 32, 425-429. DOI: 10.21873/invivo.11257.