Research group for synthesis and chemical analysis

The foundations of electrochemistry were laid almost a hundred years ago. The invention of polarography brought the Czech scientist Jaroslav Heyrovský the award of the Nobel Prize. Thanks to this fact, but not only, electrochemistry can look back at a great tradition in the Czech Republic.

Our laboratory is well adapted for the electrochemical detection of nucleic acids (DNA, RNA, including the small RNA and single nucleotides), proteins and peptides containing thiol groups (metallo-thionein) and a number of elements including heavy metals or semi-metals (such as Cd, Pb, Cu, Zn, Cr, Ti, Hg, Ag, Fe, As, Te and Se). The detection of the investigated substances is carried on both by the classical methods of polarography and by those utilizing solid or pasty electrodes.

The conjugation with electroactive markers (such as the process on the basis of quantum dots) can be used for substantially increasing the response of the analyte.  

Among further domains of our interest also the development of sensors and biosensors should be mentioned (detection of platinum cytostatics, the study of DNA methylation in correlation with the origin of tumour diseases, determining the contamination of the environment by heavy metals etc.).





RNDr. Lukáš Richtera, Ph.D.

Head - Research group for synthesis and chemical analysis
Assistant Professor


Phone: 420 545 133 311
Address: ÚCB AF, Zemědělská 1, 61300 Brno - Budova D
Office: BA02N2016
E-mail: oliver@centrum.cz
Researcher ID: N-9991-2014
ORCID: 0000-0002-8288-3999
Scopus ID: 12040049600

Team members

  • RNDr. Lukáš Richtera, Ph.D.
  • M.Sc. Amirmansoor Ashrafi, Ph.D.
  • Ing. Zuzana Bytešníková, Ph.D.
  • Ing. Zuzana Koudelková, Ph.D.
  • Ing. Vedran Milosavljević, Ph.D.
  • Ing. Pavel Švec, Ph.D.
  • M.Sc. Milica Gagič
  • Ing. Petr Mareček, Ph.D.
  • M.Sc. Atripan Mukherjee
  • Ing. Eliška Sedláčková
  • Ing. Veronika Vaňová
  • Bc. Viktor Jankovský
  • Eliška Birgusová

Topics of Ph.D. theses

  • Nanomateriály pro aplikaci v zemědělské praxi
  • Development of microfluidic Lab-on-a-chip exploiting magnetic nanoparticles for biogenic amines
  • Electrochemical detection of bacteria using the nanomaterials
  • Aplikace kovových nanočástic na patogenní mikroorganismy
  • Synthesis of potential antimicrobial peptides and study of bacterial resistance mechanisms and enzymatic activity
  • Aplikace kovových nanočástic na patogenní mikroorganismy
  • Synthesis of potential antimicrobial peptides and study of bacterial resistance mechanisms and enzymatic activity
  • Využití tištěných senzorů pro biologické aplikace
  • Nanomateriály pro aplikaci v zemědělské praxi
  • Label-free electrochemical aptasensor for detection of cancer biomarker
  • Development of microfluidic Lab-on-a-chip exploiting magnetic nanoparticles for biogenic amines
  • Využití tištěných senzorů v průtokových elektrochemických analýzách


Instruments



Projects

  • MŠMT: . 2018-2022.


Publications

  • Durovic, A.; Stojanovic, Z.; Kravic, S.; Kos, J.; Richtera, L.; Electrochemical Determination of Vitamin D3 in Pharmaceutical Products by Using Boron Doped Diamond Electrode . Electroanalysis 2019, in press, . DOI: 10.1002/elan.201900532 .
  • Bytesnikova, Z.; Richtera, L.; Smerkova, K.; Adam, V.; The graphene oxide as a tool for antibiotic resistance genes removal: A review. Environ. Sci. Pollut. Res. 2019, 26, 20148-20163. DOI: 10.1007/s11356-019-05283-y.
  • Jamroz, E.; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Makarewicz, M.; Development of furcellaran-gelatin films with Se-AgNPs as an active packaging system for extension of mini kiwi shelf life. Food Packaging Shelf 2019, 21, 1-9. DOI: 10.1016/j.fpsl.2019.100339.
  • Jamroz, E.; Kulawik, P.; Kopel, P.; Balkova, R.; Hynek, D.; Bytesnikova, Z.; Gagic, M.; Milosavljevic, V.; Adam, V.; Intelligent and active composite films based on furcellaran: Structural characterization, antioxidant and antimicrobial activities. Food Packaging Shelf 2019, 22, 1-10. DOI: 10.1016/j.fpsl.2019.100405 .
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Petrovic, S.; Guzsvany, V.; Rankovic, N.; Beljin, J.; Roncevic, S.; Dalmacija, B.; Ashrafi, A. M.; Konya, Z.; Svancara, I.; Vytras, K.; Trace level voltammetric determination of Zn(II) in selected nutrition related samples by bismuth-oxychloride-multiwalled carbon nanotube composite based electrode. Microchemical Journal 2019, 146, 178-186. DOI: 10.1016/j.microc.2018.12.053.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Gagic, M.; Jamroz, E.; Krizkova, S.; Milosavljevic, V.; Kopel, P.; Adam, V.; Current trends in detection of histamine in food and beverages . J. Agric. Food Chem. 2019, 67, 773-783. DOI: 10.1021/acs.jafc.8b05515.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.; Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Koudelkova, Z.; Bytesnikova, Z.; Xhaxhiu, K.; Kremplova, M.; Hynek, D.; Adam, V.; Richtera, L.; Electrochemical evaluation of selenium (IV) removal from its aqueous solutions by unmodified and modified graphene oxide. Molecules 2019, 24, 1-13. DOI: 10.3390/molecules24061063.
  • Ashrafi, A. M.; Richtera, L.; Preparation and characterization of carbon paste electrode bulk-modified with multiwalled carbon nanotubes and its application in a sensitive assay of antihyperlipidemic simvastatin in biological samples. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24122215.
  • Ashrafi, A. M.; Sys, M.; Sedlackova, E.; Farag, A. S.; Adam, V.; Pribyl, J.; Richtera, L.; Application of the enzymatic electrochemical biosensors for monitoring non-competitive inhibition of enzyme activity by heavy metals. Sensors 2019, 19, 1-15. DOI: 10.3390/s19132939.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Jamr; Kopel, P.; Juszczak, L.; Kawecka, A.; Bytesnikova, Z.; Milosavljevic, V.; Kucharek, M.; Makarewicz, M.; Adam, V.; Development and characterisation of furcellaran-gelatin films containing SeNPs and AgNPs that have antimicrobial activity. Food Hydrocolloids 2018, 83, 9-16. DOI: 10.1016/j.foodhyd.2018.04.028.
  • Merkel, ; Ashrafi, A; E; Bipolar membrane electrodialysis assisted pH correction of milk whey. J. Membr. Sci. 2018, 555, 185-196. DOI: 10.1016/j.memsci.2018.03.035.