Laboratory of electrochemistry

The main aim of this research group is to focused on the sensors and biosensors frabrication for detection and monitoring of biological substances. Moreover our research interest is focused on the methylated DNA by electrochemical impedance spectroscopy. In addition we are able to detect various cancer proteins or markers. We coolaborate with a research group of molecular biology that focuses on platinum cytostatics determination. Another area of interest is electrochemical detection of heavy metals, focusing on environmental protection (detection of heavy metals in waste water).

Laboratoř elektrochemie

Our research interest

  • Electrochemical detection of nucleic aicid (cancer biomarkers), study of DNA methylation
  • Electrochemical detection of proteins and peptides with thiol groups (metallothionein)
  • Electrochemical detection of metals and semi-conductors (protect of enviromental and platinium cytostatic determination)
  • Fabrication of sensors and biosensors using of nanomaterials
  • Measurement and modification of soil electrodes (glassy carbon, gold, boron doped diamond, carbon paste, screen printed) mercury dropped electrode measurement
  • Nanomaterials characterization
  • Maping of surface materials by electrochemical scanning microscope

RNDr. Lukáš Richtera, Ph.D.

Head – Research group for synthesis and chemical analysis
Assistant Professor

Phone: 420 545 133 311
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N2016
Email: oliver@centrum.cz
Researcher ID: N-9991-2014
ORCID: 0000-0002-8288-3999
Scopus ID: 12040049600

Team members

  • MSc. Amirmansoor Ashrafi, Ph.D.
  • MSc. Atripan Mukherjee, Ph.D.
  • Ing. Eliška Birgusová
  • Sanam Garehbaghi, MSc.
  • MSc. Arezoo Saadati
  • MSc. Aydan Karakaya

Topics of Ph.D. theses

  • Electrochemical detection of bacteria using the nanomaterials
  • Development of microfluidic Lab-on-a-chip exploiting magnetic nanoparticles for biogenic amines
  • Synthesis of potential antimicrobial peptides and study of bacterial resistance mechanisms and enzymatic activity
  • Label-free electrochemical aptasensor for detection of cancer biomarker

Publications

  • ASHRAFI, A.; BYTESNIKOVA, Z.; BAREK, J.; RICHTERA, L.; ADAM, V. A Critical Comparison of Natural Enzymes and Nanozymes in Biosensing and Bioassays. Biosens. Bioelectron., 2021, vol. 192. p. 1-18. ISSN 0956-5663. IF 10.618
  • ASSERGHINE, A.; ASHRAFI, A.; MUKHERJEE, A.; PETRLAK, F.; HEGER, Z.; SVEC, P.; RICHTERA, L.; NAGY, L.; SOUTO, R. M.; NAGY, G.; ADAM, V. In situ investigation of the cytotoxic and interfacial characteristics of titanium when galvanically coupled with magnesium using scanning electrochemical microscopy. ACS Appl. Mater. Interfaces, 2021, vol. 13. p. 43587-43596. ISSN 1944-8244. IF 9.229
  • BENES, J.; TODOROVSKA, V.; BUDILOVA, K.; KOVARNIK, J.; PAVELKA, J.; ATANASOSKA, N.; BUMEL, J.; FLORENZANO, A.; MAJEROVICOVA, T.; VONDROVSKY, V.; PTAKOVA, M.; BEDNAR, P.; RICHTERA, L.; KUCERA, L. What about dinner? Chemical and microresidue analysis reveals the function of late neolithic ceramic pans. Molecules, 2021, vol. 26. p. 1-16. ISSN 1420-3049. IF 4.411
  • BYTESNIKOVA, Z.; ADAM, V.; RICHTERA, L. Graphene oxide as a novel tool for mycotoxin removal. Food Control, 2021, vol. 121. p. 1-9. ISSN 0956-7135. IF 5.548
  • KUCERA, L.; ROZSYPAL, J.; BEDNAR, P.; BREZINA, M.; KALINA, L.; BEZDICKA, P.; MASLAN, M.; RICHTERA, L. “Gold corrosion”: An alternative source of red stains on gold coins. Materialia, 2021, vol. 15. p. 1-8. ISSN 2589-1529. IF 0.000
  • MITREVSKA, K.; MILOSAVLJEVIC, V.; GAGIC, M.; RICHTERA, L.; ADAM, V. 2D transition metal dichalcogenide nanomaterial-based miRNA biosensors. Appl. Mater. Today, 2021, vol. 23. p. 1-23. ISSN 2352-9407. IF 10.041
  • MUKHERJEE, S.; BOUTANT, E.; REAL, E.; MELY, Y.; ANTON, H. Imaging viral infection by fluorescence microscopy: Focus on HIV-1 early stage. Viruses-Basel, 2021, vol. 13. p. 1-21. ISSN 1999-4915. IF 5.048
  • NADTOCHAEVA, P.; BADINOVA, E.; RICHTERA, L.; VOBĚRKOVÁ, S. Crude laccase-based biosensor for the detection of catechin. Biotechnology & Biotechnological Equipment, 2021, vol. 35. ISSN 1310-2818. IF 1.452
  • PECENKA, J.; BYTESNIKOVA, Z.; KISS, M.; PENAZOVA, E.; BARANEK, M.; EICHMEIER, A.; TEKIELSKA, D.; RICHTERA, L.; POKLUDA, R.; ADAM, V. Silver nanoparticles eliminate Xanthomonas campestris pv. campestris in cabbage seeds more efficiently than hot water treatment. Mater. Today Commun., 2021, vol. 27. p. 1-8. ISSN 2352-4928. IF 3.383
  • STEPANKOVA, H.; SWIATKOWSKI, M.; KRUSZYNSKI, R., .; SVEC, P.; MICHALKOVA, H.; SMOLIKOVA, V.; RIDOSKOVA, A.; SPLICHAL, Z.; MICHALEK, P.; RICHTERA, L.; KOPEL, P.; ADAM, V.; HEGER, Z.; REX, S. The anti-proliferative activity of coordination compound-based ZnO nanoparticles as a promising agent against triple negative breast cancer cells. Int. J. Nanomed., 2021, vol. 2021. p. 4431-4449. ISSN 1178-2013. IF 6.400
  • SYS, M.; MUKHERJEE, A.; JASHARI, G.; ADAM, V.; ASHRAFI, A.; NOVAK, M.; RICHTERA, L. Bis(2,2′-bipyridil)Copper(II) Chloride Complex: Tyrosinase Biomimetic Catalyst or Redox Mediator? Materials, 2021, vol. 14. p. 1-18. ISSN 1996-1944. IF 3.623
  • VANOVA, V.; MITREVSKA, K.; MILOSAVLJEVIC, V.; HYNEK, D.; RICHTERA, L.; ADAM, V. Peptide-based electrochemical biosensors utilized for protein detection. Biosens. Bioelectron., 2021, vol. 180. p. 1-19. ISSN 0956-5663. IF 10.618
  • Durovic, A.; Stojanovic, Z.; Kravic, S.; Kos, J.; Richtera, L.; Electrochemical Determination of Vitamin D3 in Pharmaceutical Products by Using Boron Doped Diamond Electrode . Electroanalysis 2019, in press, . DOI: 10.1002/elan.201900532 .
  • Bytesnikova, Z.; Richtera, L.; Smerkova, K.; Adam, V.; The graphene oxide as a tool for antibiotic resistance genes removal: A review. Environ. Sci. Pollut. Res. 2019, 26, 20148-20163. DOI: 10.1007/s11356-019-05283-y.
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Petrovic, S.; Guzsvany, V.; Rankovic, N.; Beljin, J.; Roncevic, S.; Dalmacija, B.; Ashrafi, A. M.; Konya, Z.; Svancara, I.; Vytras, K.; Trace level voltammetric determination of Zn(II) in selected nutrition related samples by bismuth-oxychloride-multiwalled carbon nanotube composite based electrode. Microchemical Journal 2019, 146, 178-186. DOI: 10.1016/j.microc.2018.12.053.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Koudelkova, Z.; Bytesnikova, Z.; Xhaxhiu, K.; Kremplova, M.; Hynek, D.; Adam, V.; Richtera, L.; Electrochemical evaluation of selenium (IV) removal from its aqueous solutions by unmodified and modified graphene oxide. Molecules 2019, 24, 1-13. DOI: 10.3390/molecules24061063.
  • Ashrafi, A. M.; Richtera, L.; Preparation and characterization of carbon paste electrode bulk-modified with multiwalled carbon nanotubes and its application in a sensitive assay of antihyperlipidemic simvastatin in biological samples. Molecules 2019, 24, 1-14. DOI: 10.3390/molecules24122215.
  • Ashrafi, A. M.; Sys, M.; Sedlackova, E.; Farag, A. S.; Adam, V.; Pribyl, J.; Richtera, L.; Application of the enzymatic electrochemical biosensors for monitoring non-competitive inhibition of enzyme activity by heavy metals. Sensors 2019, 19, 1-15. DOI: 10.3390/s19132939.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Merkel, ; Ashrafi, A; E; Bipolar membrane electrodialysis assisted pH correction of milk whey. J. Membr. Sci. 2018, 555, 185-196. DOI: 10.1016/j.memsci.2018.03.035.
  • Jimenez Jimenez, A. M.; Moulick, A.; Richtera, L.; Krejcova, L.; Kalina, L.; Datta, R.; Svobodova, M.; Hynek, D.; Masarik, M.; Heger, Z.; Adam, V.; Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. Sens. Actuator B-Chem. 2018, 258, 295-303. DOI: 10.1016/j.snb.2017.11.074.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Moulick, A.; Heger, Z.; Milosavljevic, V.; Richtera, L.; Barroso; Merlos, M. A. R.; Buchtelova, H.; Podgajny, R.; Hynek, D.; Kopel, P.; Adam, V.; Real-time visualization of cell membrane damage using gadolinium Schiff base complex-doped quantum dots. ACS Appl. Mater. Interfaces 2018, 10, 35859-35868. DOI: 10.1021/acsami.8b15868.
  • Stojanovic, Z. S.; Koudelkova, Z.; Sedlackova, E.; Hynek, D.; Richtera, L.; Adam, V.; Determination of chromium(VI) by anodic stripping voltammetry using a silver-plated glassy carbon electrode. Anal. Methods 2018, 10, 2917-2923. DOI: 10.1039/c8ay01047a.
  • Kucera, L.; Richtera, L.; Zmrzly, M.; Jarosova, M.; Kucerova, P.; Bednar, P; Determination of the fineness of medieval coins – Evaluation of methods in a case study of a medieval pfennig. Archaeometry 2018, 60, 325-341. DOI: 10.1111/arcm.12313.
  • Ashrafi, A. M.; Koudelkova, Z.; Sedlackova, E.; Richtera, L.; Adam, V.; Review-Electrochemical sensors and biosensors for determination of mercury ions . Journal of the Electrochemical Society 2018, 165, B824-B834. DOI: 10.1149/2.0381816jes.
  • Buchtelova, H.; Strmiska, V.; Skubalova, Z.; Dostalova, S.; Michalek, P.; Krizkova, S.; Hynek, D.; Kalina, L.; Richtera, L.; Moulick, A.; Adam, V.; Heger, Z.; Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping. J. Nanobiotechnol. 2018, 16, 1-14. DOI: 10.1186/s12951-018-0369-7.