Laboratory of natural product analysis

Our laboratory focuses mainly on application of different mass spectrometric methods together with many interdependent analytical tools in unique ways to address a specific clinical problem that is not sufficiently addressed by the existing automated platforms.We develop and optimize methods using one- and two-dimensional gas chromatography-mass spectrometry (GC and GCxGC-MS) for characterization of volatile metabolites/markers produced by cell cultures, tissues, fungi, insects and other biological samples such as human breath. Furthermore, we apply matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) methods for mapping of biomolecules in diverse biological samples such as tissues, insect cuticle, etc.

Research objectives

  • GC-MS and GCxGC-MS analyses of volatile compounds in clinical samples
  • GC-MS and GCxGC-MS analyses of volatile components produced by insects and fungi
  • MALDI-MSI of biological tissues

 

Collaborations

  • International Atomic Energy Agency, Austria
  • Max Planck Institute for Chemical Ecology, Germany
  • University of Debrecen, Hungary
  • Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic
  • St Anne’s University Hospital Brno-International Clinical Research Centre, Czech Republic
  • Institute of Animal Physiology and Genetics CAS, Czech Republic
  • Brno University of Technology, Czech Republic
  • Masaryk University, Czech Republic

Vaculovicova marketa

doc. Mgr. Markéta Vaculovičová, Ph.D.

Head – Research Group for Instrumental Analysis and Bioanalysis
Academic staff – Associate Professor

Phone: 420545 13 32 90
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3010
Email: Marketa.Ryvolova@seznam.cz
Researcher ID: E-5583-2016
ORCID: 0000-0002-6771-1304

Team members

  • Mgr. Dagmar Štěrbová, Ph.D.
  • Ing. Kristýna Pavelicová
  • Ing. Kristýna Zemánková
  • Mgr. Tomáš Rýpar

Topics of Ph.D. theses

  • Development of novel approaches for clinical diagnostics
  • Design and development of biosensors for rapid and use-friendly diagnostics
  • Optional topic

 

Projects

  • International Atomic Energy Agency (IAEA), Coordinated Research Project (CRP) entitled Cuticular Hydrocarbons: Towards New Chemotaxonomic and Chemo-Ecological Tools to Manage Zeugodacus and Bactrocera Pests? (IAEA Research Contract No. 23126/R0). Duration: 2018-2023.
  • Ministry of Health of the Czech Republic (AZV) with project Central role of monocyte metabolic signature and transcription factors activity in sepsis progression (NV18-06-00529). Duration: 2018-2021.

Patents

  • Do Nascimento RR, Vaníčková L, Mendonça AL, Galindo FCA, Tavares RF, Da Silva CE, Ferreira LL, De Aquino NC, Santos JL. 2016. Formulações sintéticas com atividade de cairomônio a partir de compostos voláteis emitidos por frutos Psidium guajava (goiaba), eficazes no controle da mosca-da-fruta Sul Americana, Anastrepha fraterculus. Universidade Federal de Alagoas, Maceio Brazil. Patent No. BR 10 2016 003821 9. Patent deposited on: 23/02/2016
  • Do Nascimento RR, Vaníčková L, Mendonça AL, Ferreira LL, De Aquino NC, Tavares RF, Milet-Pinheiro P. 2015. Formulações com atividade de feromônio sexual, eficazes no controle da mosca-das-frutas Anastrepha fraterculus. Universidade Federal de Alagoas, Maceio Brazil. Patent No. BR 10 2015 016404 1. Patent deposited on: 08/07/2015
  • Pires EP, Mendonça AL, Santos RS, Vaníčková L,  Sant´Ana AEG, Do Nascimento RR. 2014. Composição com atividade feromonal e seu uso no controle da broca de frutos de Anonaceas. Universidade Federal de Alagoas, Maceio Brazil. Ptanet No. BR 10 2014 010066 0.

Publications

  • NUCHTAVORN, N.,RYPAR, T.,NEJDL, L.,VACULOVICOVA, M.,MACKA, M. Distance-based detection in analytical flow devices: From gas detection tubes to microfluidic chips and microfluidic paper-based analytical devices TrAC – Trends in Analytical Chemistry. 2022, in press.
  • VANÍČKOVÁ, L.,DO, T.,VEJVODOVÁ, M.,HORÁK, V.,HUBÁLEK, M.,EMRI, G.,ZEMÁNKOVÁ, K.,PAVELICOVÁ, K.,KŘÍŽKOVÁ, S.,FALTUSOVÁ, V.,POMPEIANO, A.,VACULOVIČOVÁ, M.,ZÍTKA, O.,VACULOVIČ, T.,ADAM, V. Mapping of melim melanoma combining icp-ms and maldi-msi methods. International Journal of Biological Macromolecules. 2022, vol. 203. no., pp. 583-592.
  • Scolari, F., Valerio, F., Benelli, G., Papadopoulos, N. T. & Vanickova, L. Tephritid fruit fly semiochemicals: current knowledge and future perspectives Insects 12, 1-57, doi:https://doi.org/10.3390/insects12050408 (2021).
  • Vanickova, L., Pompeiano, A., Madera, P., Massad, T. J. & Vahalik, P. Terpenoid profiles of resin in the genus Dracaena are species specific. Phytochemistry 170, 1-8, doi:10.1016/j.phytochem.2019.112197 (2020).
  • Vahalik, P. et al. The conservation status and population mapping of the endangered Dracaena serrulata in the Dhofar mountains, Oman. Forests 11, 1-16, doi:10.3390/f11030322 (2020).
  • Vahalik, P. et al. Metrics of growth habit derived from the 3D tree point cloud used for species determination-A new approach in botanical taxonomy tested on Dragon tree group example. Forests 11, 1-14, doi:10.3390/f11030272 (2020).
  • Madera, P. et al. What we know and what we do not know about Dragon trees? Forests 11, 1-34, doi:10.3390/f11020236 (2020).