Laboratory of nanomedicine

In its research, the Laboratory of Nanomedicine focuses on the formulation of nanoparticles based on biomaterials and their detailed characterization for therapeutic use in the treatment of cancer and infectious diseases. We concentrate on ensuring that the prepared transport nanosystems are fully biologically functional, which can only be achieved by meeting strict physical and chemical criteria. Therefore, in addition to their size and shape, we take into account the nature of the therapeutic substance enclosed in the carrier, the mechanism of its encapsulation and we also deal with the appropriate modification of surface properties of nanotransporters.

Laboratoř nanomedicíny

The main research focus of the laboratory

  • Optimization of production of different types of recombinant protein carriers (apoferritins)
  • Study of the process of encapsulation of bioactive molecules with antitumor effect into apoferritin nanostructures and their surface modification
  • Use of apoferritin and other nanoparticles based on biomaterials in bioorthogonal chemistry
  • Formulation of lipid nanoparticles carrying RNA-based therapeutics for the treatment and prevention of infectious diseases (e.g. hepatitis B, tick-borne encephalitis)
  • Formulation of lipid nanoparticles with application in contrast imaging and for theranostic purposes
  • Nanotools for transport CRISPR-Cas9

 

Ing. Zdeněk Kratochvíl

Head – Laboratory of nanomedicine
Academic staff – assistant

Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3016
Email: zdenekkratochvil14@gmail.com

Team members

  • Ing. Zdeněk Kratochvíl
  • Ing. Michaela Vojníková
  • Alžběta Ressnerová, M.Sc.
  • Mgr. Paulína Takácsová
  • Ing. Kateřina Kapołková
  • Bc. Eliška Gruberová
  • Lucie Marková

Topics of Ph.D. theses

  • Optional topic
  • Volné téma – v případě zájmu kontaktujte vedoucího výzkumné skupiny

Publications

  • STEPANKOVA, H.; SWIATKOWSKI, M.; KRUSZYNSKI, R., .; SVEC, P.; MICHALKOVA, H.; SMOLIKOVA, V.; RIDOSKOVA, A.; SPLICHAL, Z.; MICHALEK, P.; RICHTERA, L.; KOPEL, P.; ADAM, V.; HEGER, Z.; REX, S. The anti-proliferative activity of coordination compound-based ZnO nanoparticles as a promising agent against triple negative breast cancer cells. Int. J. Nanomed., 2021, vol. 2021. p. 4431-4449. ISSN 1178-2013. IF 6.400
  • HAMEDUH, T.; MOKRY, M.; MILLER, A. D.; ADAM, V.; HEGER, Z.; HADDAD, Y. A rotamer relay information system in the epidermal growth factor receptor–drug complexes reveals clues to new paradigm in protein conformational change. Comp. Struct. Biotechnol. J., 2021, vol. 19. p. 5443-5454. ISSN 2001-0370. IF 7.271
  • RESSNEROVA, A.; NOVOTNY, F.; MICHALKOVA, H.; PUMERA, M.; ADAM, V.; HEGER, Z. Efficient protein transfection by swarms of chemically powered plasmonic virus-sized nanorobots. ACS Nano, 2021, vol. 15. p. 12899-12910. ISSN 1936-0851. IF 15.881
  • JAKLOVA, K.; FEGLAROVA, T.; REX, S.; HEGER, Z.; ECKSCHLAGER, T.; HRABETA, J.; HODEK, P.; KOLARIK, M.; INDRA, R. Apoferritin/Vandetanib Association Is Long-Term Stable But Does Not Improve Pharmacological Properties of Vandetanib. Int. J. Mol. Sci., 2021, vol. 22. p. 1-12. ISSN 1422-0067. IF 5.923
  • Skubalova, Z.; Michalkova, H.; Michalek, P.; Strmiska, V.; Guran, R.; Merlos, M. A. R.; Castkova, K.; Hynek, D.; Pekarik, V.; Zitka, O.; Adam, V.; Heger, Z.; Prevalent anatase crystalline phase increases the cytotoxicity of biphasic titanium dioxide nanoparticles in mammalian cells. Colloid Surf. B-Biointerfaces 2019, 182, 1-10. DOI: 1.
  • Haddad, Y.; Adam, V.; Heger, Z.; Rotamer dynamics: analysis of rotamers in molecular dynamics simulations of proteins . Biophys. J. 2019, 116, 2062-2072. DOI: 10.1016/j.bpj.2019.04.017.
  • Tesarova, B.; Charousova, M.; Dostalova, S.; Bienko, A.; Kopel, P.; Kruszynski, R.; Hynek, D.; Michalek, P.; Eckschlager, T.; Stiborova, M.; Adam, V.; Heger, Z.; Folic acid-mediated re-shuttling of ferritin receptor specificity towards a selective delivery of highly cytotoxic nickel(II) coordination compounds. Int. J. Biol. Macromol. 2019, 126, 1099-1111. DOI: 10.1016/j.ijbiomac.2018.12.128.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Michalkova, H.; Strmiska, V.; Kudr, J.; Skubalova, Z.; Tesarova, B.; Svec, P.; Richtera, L.; Zitka, O.; Adam, V.; Heger, Z.; Tuning the surface coating of IONs towards efficient sonochemical tethering and sustained liberation of topoisomerase II poisons. Int. J. Nanomed. 2019, 14, 7609. DOI: 10.2147/IJN.S208810.
  • Al Zoubi, M. S.; Al-Batayneh, K. M.; Al-Trad, B.; Haddad, Y.; Hussein, E.; Al Khateeb, W.; Aljabali, A. A. A.; Bodoor, K.; Shehab, M.; Al Hamad, M.; Muhaidat, R.; Evaluation of vitamin B12, folate and ferritin serum levels in Jordanian population. J. Nutr. Sci. Vitaminol. 2019, 65, 309-317. DOI: 10.3177/jnsv.65.309.
  • Indra, R.; Pompach, P.; Martinek, V.; Takacsova, P.; Vavrova, K.; Heger, Z.; Adam, V.; Eckschlager, T.; Kopeckova, K.; Arlt, V. M.; Stiborova, M.; Identification of human enzymes oxidizing the anti-thyroid-cancer drug vandetanib and explanation of the high efficiency of cytochrome P450 3A4 in its oxidation . Int. J. Mol. Sci. 2019, 20, 1-22. DOI: 10.3390/ijms20143392.
  • Alves, M. N.; Nesterenko, P. N.; Paull, B.; Haddad, P. R.; Macka, M.; Separation of superparamagnetic magnetite nanoparticles by capillary zone electrophoresis using non-complexing and complexing electrolyte anions and tetramethylammonium as dispersing additive. Electrophoresis 2018, 39, 1429-1436. DOI: 10.1002/elps.201800095.
  • Kynicky, J.; Milosavljevic, V.; Jelinkova, P.; Haddad, Y.; Merlos, M. A. R.; Buchtelova, H.; Bytesnikova, Z.; Brtnicky, M.; Richtera, L.; Kopel, P.; Adam, V.; Europium and terbium Schiff base peptide complexes as potential antimicrobial agents against Salmonella typhimurium and Pseudomonas aeruginosa. Chem. Pap. 2018, 72, 1437–1449. DOI: 10.1007/s11696-018-0400-3.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Dostalova, S.; Polanska, H.; Svobodova, M.; Balvan, J.; Krystofova, O.; Haddad, Y.; Krizkova, S.; Masarik, M.; Eckschlager, T.; Stiborova, M.; Heger, Z.; Adam, V.; Prostate-specific membrane antigen-targeted site-directed antibody-conjugated apoferritin nanovehicle favorably influences in vivo side effects of doxorubicin . Sci. Rep. 2018, 8, 1-13. DOI: 10.1038/s41598-018-26772-z.
  • Buchtelova, H.; Strmiska, V.; Skubalova, Z.; Dostalova, S.; Michalek, P.; Krizkova, S.; Hynek, D.; Kalina, L.; Richtera, L.; Moulick, A.; Adam, V.; Heger, Z.; Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping. J. Nanobiotechnol. 2018, 16, 1-14. DOI: 10.1186/s12951-018-0369-7.
  • Jelinkova, P.; Splichal, Z.; Jimenez Jimenez, A. M.; Haddad, Y.; Mazumdar, A.; Sur, V. P.; Milosavljevic, V.; Kopel, P.; Buchtelova, H.; Guran, R.; Zitka, O.; Richtera, L.; Hegerova, D.; Heger, Z.; Moulick, A.; Adam, V.; Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus. Infect. Drug Resistance 2018, 2018, 1807. DOI: 10.2147/IDR.S160975.
  • Bodoor, K.; Melhem, D.; Shotar, A.; Alkhatib, A.; Batiha, O.; Abu-Sheikha, A.; Haddad, Y.; Prevalence of cervical human papillomavirus is associated with p16 expression and irregular menstrual cycles among married Jordanian women. European Journal of Gynaecological Oncology 2018, 39, 779-785. DOI: 10.12892/ejgo4318.2018.
  • Moulick, A.; Richtera, L.; Milosavljevic, V.; Cernei, N.; Haddad, Y.; Zitka, O.; Kopel, P.; Heger, Z.; Adam, V.; Advanced nanotechnologies in avian influenza: Current status and future trends – A review . Analytica Chimica Acta 2017, 983, 42-53 . DOI: 10.1016/j.aca.2017.06.045.
  • Bodoor, K.; Al-Ghabkari, A.; Matalka, I.; Haddad, Y.; Alkhateeb, A.; Jaradat, S.; Jaradat, Z.W.; Al-Ghazo, M.; Abu-Sheikha, A.; Abu Jalboush, S; Jarun, Y.; Assessment of p53 mutations, expression and prognosis in bladder cancer patients from Jordan: Identification of novel deletion mutations in the DNA-binding domain. Meta Gene 2017, 12, 33-42. DOI: 10.1016/j.mgene.2017.01.002.
  • Milosavljevic, V.; Jelinkova, P.; J; Moulick, A.; Haddad, Y.; Buchtelova, H.; Krizkova, S.; Heger, Z.; Kalina, L.; Richtera, L.; Kopel, P.; Adam, V.; Alternative synthesis route of biocompatible polyvinylpyrrolidone nanoparticles and their effect on pathogenic microorganisms. Molecular Pharmaceutics 2017, 14, 221-233. DOI: 10.1021/acs.molpharmaceut.6b00807.
  • Anyz, J.; Vyslouzilova, L.; Vaculovic, T.; Tvrdonova, M.; Kanicky, V.; Haase, H.; Horak, V.; Stepankova, O.; Heger, Z.; Adam, V.; Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Sci. Rep. 2017, 7, 1-13. DOI: 10.1038/srep40169.
  • Haddad, Y.; Heger, Z.; Adam, V.; Targeting Neuroblastoma Cell Surface Proteins: Recommendations for Homology Modeling of hNET, ALK, and TrkB. Front. Molec. Neurosci. 2017, 10, 1-7. DOI: 10.3389/fnmol.2017.00007.