Laboratory of Environmental Chemistry and Enzymology

The Laboratory of Environmental Chemistry and Enzymology focuses on the use of various types of microorganisms (fungi, bacteria and yeasts) and their secondary metabolites in the field of environmental protection.

The important part of the laboratory is The Culture Collection of Basidiomycetes, which are studied as new sources of secondary metabolites characterized by various activities (antimicrobial, antifungal, antioxidant, etc.) with potential use in the pharmaceutical industry as a new source of natural substances with biological activity.

The aim of the research is to provide an effective alternative in the form of environmentally safe substances and thus reduce the burden on the environment.

Another part of the research is focused on the study of production and immobilization of biotechnologically important enzymes whose application potential is tested in a bioreactor on real samples of water contaminated by drug residues or synthetic dyes etc.

Another area of ​​interest is the characterization and study of microorganisms in real conditions such as forest or farmland, compost, food waste, wastewater or sediment with regard to their use, for example in bioremediation, biodegradation, soil quality improvement or soil regeneration.

Our research group is also involved in studying the process of composting and treating food waste. We analyze these biological materials using valid standards, and the scope of analysis depends on the origin and planned use of compost and bio-waste. In the laboratory, we have several types of composters (electric, rotary) of various sizes and a destructor. Furthermore, we also assess the quality of decomposition and the compost hygiene process using molecular biological methods (sequencing) or by determining enzymatic activities.


Photo

Ing. Martina Vršanská, Ph.D.

Head of Laboratory of environmental chemistry and enzymology


Phone: +420 734 252 656
Address: ÚCB AF, třída Generála Píky 1999/5, 61300 Brno – Building K
Office: BA02N3018
Email: martina.vrsanskav@mendelu.cz


Team members

Topics of Ph.D. theses

  • Optional topic

Topics of bachelor theses

  • Verification of the effect of natural antimicrobial substances on inhibiting the growth of pathogenic microorganisms.
  • Study of composting gastro waste using molecular biology methods.
  • Immobilization of ligninolytic enzymes produced by basidiomycete fungi.

Bachelor thesis

  • Oleksandra Ovchynnikova: Study of laccase production by selected species of fungi (2017)
  • Adéla Švoncová: Antibacterial effects of propolis (2018)
  • Martin Řiháček: Using of modern molecular methods for isolation and identification of ligninolytic enzymes (2018)
  • Andrea Blahutová: Degradation of synthetic dyes using Phanerochaete chrysosporium (2018)
  • Ester Badinová: Use of ligninolytic enzymes in the degradation of pollutants (2018)
  • Žaneta Nedvědická: Use of white rot mushrooms in bioremediation of pollutants (2019)
  • Polina Nadtochaeva: Use of ligninolytic enzymes in food industry (2019)
  • Petra Šťavíková: Monitoring the microbiological safety of the food waste during its processing (2022)
  • Sofiia Lymarenko: Application of the enzyme tyrosinase in the food industry (2021)
  • Kristýna Pirochtová: Monitoring of physico – chemical parameters food-waste during its processing (2022)
  • Mariia Balycheva: Influence of inoculation of Trametes versicolor and Bacillus subtilis on food waste composting efficiency (2021)
  • Jaroslava Bednářová: Determination of ergosterol in food (2022)
  • Tereza Bečicová: Determination of streptomycin in honey samples (2022)
  • Eliška Kopuncová: Determination of tetracycline in milk (2022)
  • Petra Krejčiříková: Použití dřevokazných hub na biosorpci niklu a kadmia (2022)
  • Markéta Mlčáková: Skríning a identifikace ligninolytických enzymů basidiomycetních hub izolovaných z českých lesů (2020)
  • Vendula Jemelíková: Study of laccase production by batch and continous cultivation (2021)
  • Lucie Komárková Studium změn antimikrobiální aktivity houby Ganoderma adspersum pomocí kultivační strategie OSMAC (2023)
  • Lucie Malinová Navržení vhodného bakteriálního inokula pro podporu procesu kompostování gastroodpadu (2023)
  • Paulína Bírošíková Biodegradace azobarviv působením hub bílé hniloby (2023)
  • Lenka Tomková Sledování výskytu patogenních mikroorganismů v potravinovém odpadu po přídavku různých aditiv (2023)

 

Diploma thesis

  • Nikola Schlosserová: Immobilization of lignolytic enzymes (2019)
  • Bc. Andrea Blahutová: CLEA imobilizace enzymů z hub bílé a hnědé hniloby (2020)
  • Polina Nadtochaeva: Detekce bisfenolu A jako endokrinního disruptoru živočišných organismů (2020)
  • Bc. Tereza Dufková: Stanovení kvalitativních parametrů u různých vzorků medu (2021)
  • Ing. Sofiia Lymarenko Stanovení aktivity průmyslově významných enzymů produkovaných mikroorganismy izolovanými z potravinového odpadu (2023)

 

Vedené práce

 

  • Ivana Palkechová (bakalářská práce, rok ukončení 2024): Vliv enkapsulace kmene Bacillus licheniformis na účinnost kompostování potravinového odpadu
  • Denisa Stloukalová (bakalářská práce, rok ukončení 2024): Enkapsulace vybraných druhů kvasinek produkujících lipázy
  • Vendula Nekvasilová (bakalářská práce, rok ukončení 2024): Ověření účinku přírodních antimikrobiálních látek pro inhibici růstu patogenních mikroorganismů
  • Vendulka Pauzarová (bakalářská práce, rok ukončení 2024): Imobilizace ligninolytických enzymů produkovaných basidiomycetními houbami
  • Veronika Vaníčková (bakalářská práce, rok ukončení 2024): Ověření kávové sedliny jako antimikrobiální látky pro inhibici růstu patogenních mikroorganismů
  • Bc. Petra Šťavíková (diplomová práce, rok ukončení 2024): Sledování parametrů zrání mladého bílého vína
  • Bc. Michaela Dobešová (diplomová práce, rok ukončení 2024): Ověření kávové sedliny jako potenciálního substrátu pro bakterie použitelné při bioremediaci
  • Ing. Ester Kovaříková (disertační práce, rok ukončení 2024): Studium problematiky kompostování gastroodpadu metodami molekulární biologie

 

Collaboration with:

  • Technical university in Liberec
  • Institute of Chemistry of the Slovak Academy of Sciences, Slovak Academy of Sciences, Slovak Republic
  • Center of Biological Engineering, University of Minho, Portugal
  • University of West Hungary, Hungary
  • Austria, University of Vienna, Department of Environmental Geosciences
  • Bereich Holzchemie und Biotechnologie , Team Holzchemie, Kompetenzzentrum Holz GmbH , Linz, Rakousko

Publications

2022

Fernandes Helena,; Salgado José Manuel,; Ferreira Marta,; Vršanská Martina,; Fernandes Nélson,; Castro Carolina,; Oliva-Teles Aires,; Peres Helena, Belo Isabel

Valorization of brewer's spent grain using biological treatments and its application in feeds for European seabass (Dicentrarchus labrax) Journal Article

In: Frontiers in Bioengineering and Biotechnology, vol. 10, 2022, ISSN: 2296-4185 .

Abstract | Links

Milena Maryskova,; Martina Vrsanska,; Alena Sevcu,; Vit Novotny,; Andrea Blahutova,; Stanislava Voberkova,

Laminated PAA nanofibers as a practical support for crude laccase: A new perspective for biocatalytic treatment of micropollutants in wastewaters Journal Article

In: Environmental Technology & Innovation, vol. 26, 2022, ISBN: 2352-1864.

Abstract | Links

2021

HOFMANN, T.; ALBERT, L.; NEMETH, L.; VRSANSKA, M.; SCHLOSSEROVA, N.; VOBERKOVA, S.; VISI-RAJCZI, E.

Antioxidant and Antibacterial Properties of Norway Spruce (Picea abies H. Karst.) and Eastern Hemlock (Tsuga canadensis (L.) Carrière) Cone Extracts Journal Article

In: Forests, vol. 12, iss. 9, 2021.

Abstract | Links

C. Agarwal,; T. Hofmann, and Vrsanska; N. Schlosserova,; E. Visi-Rajczi,; S. Voberkova,; Pasztory, Z.

In vitro antioxidant and antibacterial activities with polyphenolic profiling of wild cherry, the European larch and sweet chestnut tree bark Journal Article

In: European Food Research and Technology, 2021, ISSN: 2355-2370.

Abstract | Links

2020

Voběrková, Stanislava; Maxianová, Alžbeta; Schlosserová, Nikola; Adamcová, Dana; Vršanská, Martina; Richtera, Lukáš; Gagić, Milica; Zloch, Jan; Vaverková, Magdalena Daria

Food waste composting - Is it really so simple as stated in scientific literature? - A case study Journal Article

In: Sci Total Environ, vol. 723, pp. 138202, 2020, ISSN: 1879-1026.

Abstract | Links

Voběrková, Stanislava; Maxianová, Alžbeta; Schlosserová, Nikola; Adamcová, Dana; Vršanská, Martina; Richtera, Lukáš; Gagić, Milica; Zloch, Jan; Vaverková, Magdalena Daria

Food waste composting - Is it really so simple as stated in scientific literature? - A case study Journal Article

In: Sci Total Environ, vol. 723, pp. 138202, 2020, ISSN: 1879-1026.

Abstract | Links

Vaverková, Magdalena Daria; Elbl, Jakub; Voběrková, Stanislava; Koda, Eugeniusz; Adamcová, Dana; Gusiatin, Zygmunt Mariusz; Rahman, Abd Al; Radziemska, Maja; Mazur, Zbigniew

Composting versus mechanical-biological treatment: Does it really make a difference in the final product parameters and maturity Journal Article

In: Waste Manag, vol. 106, pp. 173–183, 2020, ISSN: 1879-2456.

Abstract | Links

Ananbeh, Hanadi; Rodrigo, Miguel Angel Merlos; Jelinkova, Pavlina; Strmiska, Vladislav; Splichal, Zbynek; Jehmlich, Nico; Michalkova, Hana; Stojanović, Marko; Voberkova, Stanislava; Adam, Vojtech; Moulick, Amitava

Soil protein as a potential antimicrobial agent against methicillin -resistant Staphylococcus aureus Journal Article

In: Environ Res, vol. 188, pp. 109320, 2020, ISSN: 1096-0953.

Abstract | Links

2019

Ananbeh, Hanadi; Stojanović, Marko; Pompeiano, Antonio; Voběrková, Stanislava; Trasar-Cepeda, Carmen

Use of soil enzyme activities to assess the recovery of soil functions in abandoned coppice forest systems Journal Article

In: Sci Total Environ, vol. 694, pp. 133692, 2019, ISSN: 1879-1026.

Abstract | Links

2018

Voběrková, Stanislava; Solčány, Veronika; Vršanská, Martina; Adam, Vojtěch

Immobilization of ligninolytic enzymes from white-rot fungi in cross-linked aggregates Journal Article

In: Chemosphere, vol. 202, pp. 694–707, 2018, ISSN: 1879-1298.

Abstract | Links

2017

Stanislava Voběrková,; Magdalena Dária Vaverková,; Adamcová., Dana

Enzyme Production During Composting of Aliphatic–Aromatic Copolyesters in Organic Wastes Journal Article

In: Environmental Engineering Science, vol. 34, iss. 3, 2017.

Abstract | Links

Voběrková, Stanislava; Vaverková, Magdalena D; Burešová, Alena; Adamcová, Dana; Vršanská, Martina; Kynický, Jindřich; Brtnický, Martin; Adam, Vojtěch

Effect of inoculation with white-rot fungi and fungal consortium on the composting efficiency of municipal solid waste Journal Article

In: Waste Manag, vol. 61, pp. 157–164, 2017, ISSN: 1879-2456.

Abstract | Links

Voběrková, Stanislava; Vaverková, Magdalena Dária; Adamcová, Dana

Enzyme Production During Composting of Aliphatic–Aromatic Copolyesters in Organic Wastes Journal Article

In: Environmental Engineering Science, vol. 34, no. 3, 2017.

Abstract | Links

Vršanská, Martina; Voběrková, Stanislava; Jiménez, Ana María Jiménez; Strmiska, Vladislav; Adam, Vojtěch

Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes Journal Article

In: Int J Environ Res Public Health, vol. 15, no. 1, 2017, ISSN: 1660-4601.

Abstract | Links

2016

Vrsanska, Martina; Voberkova, Stanislava; Langer, Vratislav; Palovcikova, Dagmar; Moulick, Amitava; Adam, Vojtech; Kopel, Pavel

Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes Journal Article

In: Molecules, vol. 21, no. 11, 2016, ISSN: 1420-3049.

Abstract | Links

Voběrková, Stanislava; Hermanová, Soňa; Hrubanová, Kamila; Krzyžánek, Vladislav

Biofilm formation and extracellular polymeric substances (EPS) production by Bacillus subtilis depending on nutritional conditions in the presence of polyester film Journal Article

In: Folia Microbiol (Praha), vol. 61, no. 2, pp. 91–100, 2016, ISSN: 1874-9356.

Abstract | Links