Laboratory of natural product analysis

Our laboratory focuses mainly on application of different mass spectrometric methods together with many interdependent analytical tools in unique ways to address a specific clinical problem that is not sufficiently addressed by the existing automated platforms.We develop and optimize methods using one- and two-dimensional gas chromatography-mass spectrometry (GC and GCxGC-MS) for characterization of volatile metabolites/markers produced by cell cultures, tissues, fungi, insects and other biological samples such as human breath. Furthermore, we apply matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) methods for mapping of biomolecules in diverse biological samples such as tissues, insect cuticle, etc.

Laboratoř analýzy přírodních látek

Research objectives
  • GC-MS and GCxGC-MS analyses of volatile compounds in clinical samples
  • GC-MS and GCxGC-MS analyses of volatile components produced by insects and fungi
  • MALDI-MSI of biological tissues
Collaborations
  • International Atomic Energy Agency, Austria
  • Max Planck Institute for Chemical Ecology, Germany
  • University of Debrecen, Hungary
  • Institute of Organic Chemistry and Biochemistry of the CAS, Czech Republic
  • St Anne’s University Hospital Brno-International Clinical Research Centre, Czech Republic
  • Institute of Animal Physiology and Genetics CAS, Czech Republic
  • Brno University of Technology, Czech Republic
  • Masaryk University, Czech Republic

Photo

Ing. Lucie Pompeiano Vaníčková, PhD.

Head – Laboratory of natural product


Phone: 420 545 133 352
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3012
Email:


Team members

  • Ing. Kristýna Pavelicová
  • Ing. Kristýna Zemánková

Topics of Ph.D. theses

  • Optional topic

 

Projects

  • Marie Skłodowska-Curie individual fellowship under South Moravian Programme for Distinguished Researchers (SoMoPro 3). Project: Metallothionein as Prognostic Biomarker in Skin Cancer (No. 6SA17676). Duration: 2017-2020.
  • International Atomic Energy Agency (IAEA), Coordinated Research Project (CRP) entitled Cuticular Hydrocarbons: Towards New Chemotaxonomic and Chemo-Ecological Tools to Manage Zeugodacus and Bactrocera Pests? (IAEA Research Contract No. 23126/R0). Duration: 2018-2023.
  • Ministry of Health of the Czech Republic (AZV) with project Central role of monocyte metabolic signature and transcription factors activity in sepsis progression (NV18-06-00529). Duration: 2018-2021.

Patents

  • Do Nascimento RR, Vaníčková L, Mendonça AL, Galindo FCA, Tavares RF, Da Silva CE, Ferreira LL, De Aquino NC, Santos JL. 2016. Formulações sintéticas com atividade de cairomônio a partir de compostos voláteis emitidos por frutos Psidium guajava (goiaba), eficazes no controle da mosca-da-fruta Sul Americana, Anastrepha fraterculus. Universidade Federal de Alagoas, Maceio Brazil. Patent No. BR 10 2016 003821 9. Patent deposited on: 23/02/2016
  • Do Nascimento RR, Vaníčková L, Mendonça AL, Ferreira LL, De Aquino NC, Tavares RF, Milet-Pinheiro P. 2015. Formulações com atividade de feromônio sexual, eficazes no controle da mosca-das-frutas Anastrepha fraterculus. Universidade Federal de Alagoas, Maceio Brazil. Patent No. BR 10 2015 016404 1. Patent deposited on: 08/07/2015
  • Pires EP, Mendonça AL, Santos RS, Vaníčková L,  Sant´Ana AEG, Do Nascimento RR. 2014. Composição com atividade feromonal e seu uso no controle da broca de frutos de Anonaceas. Universidade Federal de Alagoas, Maceio Brazil. Ptanet No. BR 10 2014 010066 0.

Publications

  • Vaníčková L, Pompeiano A, Maděra P, Massad TJ, Vahalík P. 2020. Terpenoid profiles of
    resin in the genus Dracaena are species specific. Phytochemistry, 170, 112197, 1-8. DOI:
    https://doi.org/10.1016/j.phytochem.2019.112197
  • Vaněčková T, Vaníčková LP, Pomorski A, Vaculovic T, Krezel A, Vaculovicova M, Adam V.
    2019. Molecularly imprinted polymers coupled to mass spectrometric detection for
    metallothionein sensing. Talanta. 198, 224-229. DOI:
    https://doi.org/10.1016/j.talanta.2019.01.089
  • Strmiska V, Michalek P, Lackova Z, Guran R, Krizkova S, Vanickova L, Zitka O, Stiborova M,
    Eckschlager T, Klejdus B, Pacik D, Tvrdikova E, Haase H, Adam V, Heger Z. 2019. Sarcosine
    is a prostate epigenetic modifier that elicits aberrant methylation patterns through the SAMe-
    Dnmts axis. Molecular Oncology. 13 (5) 1002-1017. DOI: 10.1002/1878-0261.12439
  • Vanickova L, Guran R, Kollar S, Emri G, Krizkova S, Do T, Zitka O, Heger Z, Adam V. 2019.
    Mass spectrometric imaging of cysteine rich proteins in human skin. International Journal of
    Biological Macromolecules. 125, 270-277. DOI: https://doi.org/10.1016/j.ijbiomac.2018.11.272
  • Tvrdoňová M, Vlčnovská M, Vaníčková LP, Kanicky V, Adam V., Ascher L., Jakubowski N,
    Vaculovicova M, Vaculovic T. 2019. Gold nanoparticles as labels for immunochemical
    analysis using laser ablation inductively coupled plasma mass spektrometry. Analytical and
    Bioanalytical Chemistry. 411 (3), 559-564. DOI: https://doi.org/10.1007/s00216-018-1300-7
  • Guráň R, Vaníčková LP, Horák V, Křížková S, Michálek P, Heger Z, Zítka O, Adam V. 2017.
    MALDI MSI of MeLiM melanoma: Searching for differences in protein profiles. PLOS ONE.
    12:1-15. ISSN: 1932-6203. DOI: 10.1371/journal.pone.0189305
  • Michálek P, Strmiska V, Lacková Z, Křížková S, Buchtelová H, Guráň R, Vaníčková LP,
    Dostálová S, Zítka O, Adam V, Heger Z. 2017. SARDH/PIPOX converts exogenously added
    sarcosine while providing methyl groups to methyl donor S-adenosyl methionine. International
    Journal of Molecular Medicine. S54 (S54 s.)ISSN: 1107-3756. DOI: 10.1002/1878-
    0261.12439
  • Vaníčková L, Nagy R, Pompeiano A, Kalinová B. 2017. Epicuticular chemistry reinforces the
    new taxonomic classification of the Bactrocera dorsalis species complex (Diptera:
    Tephritidae, Dacinae). PLoS ONE 12(9): e0184102. DOI: https://doi.
    org/10.1371/journal.pone.0184102.
  • Vaníčková L, Canale A, Benelli G. 2017. Sexual chemoecology of mosquitoes (Diptera,
    Culicidae): Current knowledge and implications for vector control programs. Parasitology
    International. 66 (2): 190-195. DOI: 10.1016/j.parint.2016.09.010.
  • Pires EP, Mendonça AL, Vaníčková L, Serra NSJ, Da Silva RCC, Dos Santos DC, Santos RS,
    Sant´Ana AEG, Do Nascimento RR. 2016. Identification and field and laboratory tests of the
    sex attractant of the Annona fruit borer, Cerconota anonella Sepp. (Lepidoptera:
    Oecophoridae). Journal of Applied Entomology. 140:72-80. DOI:
    https://doi.org/10.1111/jen.12228
  • Campos RS, Mendonça AL, Cabral-Jr CR, Vaníčková L, Do Nascimento RR. 2016. Species-
    and cast-specific trail following behaviour in leaf-cutting ant Atta opaciceps, Borgmeier
    (Hymenoptera: Formicidae). Journal of Insect Physiology 86:25-31. DOI:
    10.1016/j.jinsphys.2015.12.008
  • De Meyer M, Delatte H, Ekesi S, Jordaens K, Kalinová B, Manrakhan A, Mwatawala M, Steck
    G, Van Cann J, Vaníčková L, Břízová R, Virgilio M. 2015. An integrative approach to unravel
    the Ceratitis FAR (Diptera: Tephtitidae) cryptic species complex: a review. Resolution of
    Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate
    International Trade. In: De Meyer M, Clarke AR, Vera TM, Hendrichs J (Eds) IAEA Special
    Issue. ZooKeys 540:405-427. DOI: 10.3897/zookeys.540.10046
  • Vaníčková L, Hernández-Ortiz V, Bravo ISJ, Dias V, Roriz AKP, Laumann RA, Mendonça
    AL, Paranhos BAJ, Do Nascimento RR. 2015. Current knowledge of the species complex
    Anastrepha fraterculus in Brazil. Resolution of Cryptic Species Complexes of Tephritid Pests
    to Enhance SIT Application and Facilitate International Trade. In: De Meyer M, Clarke AR,
    Vera TM, Hendrichs J (Eds) IAEA Special Issue. ZooKeys 540:211-237.
    DOI: 10.3897/zookeys.540.9791
  • Vaníčková L, Břízová R, Pompeiano A, Ferreira LL, de Aquino NC, Tavares RF, Rodriguez LD,
    Mendonça AL, Canal NA, Do Nascimento RR. 2015. Characterisation of the chemical profiles
    of Brazilian and Andean morphotypes belonging to the Anastrepha fraterculus complex
    (Diptera, Tephritidae). Resolution of Cryptic Species Complexes of Tephritid Pests to
    Enhance SIT Application and Facilitate International Trade. In: De Meyer M, Clarke AR, Vera
    TM, Hendrichs J (Eds) IAEA Special Issue. ZooKeys 540:193-209.
    DOI: 10.3897/zookeys.540.9649
  • Břízová R, Vaníčková L, Faťarová M, Ekesi S, Hoskovec M, Kalinová B. 2015. Pheromone
    analyses of the African fruit fly species complex (Diptera: Tephritidae). Resolution of Cryptic
    Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International
    Trade. In: De Meyer M, Clarke AR, Vera TM, Hendrichs J (Eds) IAEA Special Issue. ZooKeys
    540:385-404. DOI: DOI: 10.3897/zookeys.540.9630
  • Vaníčková L, Břízová R, Pompeiano A, Ekesi S, De Meyer M. 2015. Cuticular hydrocarbons
    corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis
    rosa) populations. Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance
    SIT Application and Facilitate International Trade. In: De Meyer M, Clarke AR, Vera TM,
    Hendrichs J (Eds) IAEA Special Issue. ZooKeys 540:507-524.
    DOI: 10.3897/zookeys.540.9619
  • Vaníčková L, Břízová R, Mendonça AL, Pompeiano A, Do Nascimento RR. 2015. Intraspecific
    variation of cuticular hydrocarbon profiles in the Anastrepha fraterculus (Diptera: Tephritidae)
    species complex. Journal of Applied Entomology. 139 (9): 679-689. DOI: 10.1111/jen.12204
  • Milet-Pinheiro P, Navarro DMA, De Aquino NC, Ferreira LL, Tavares RF, Da Silva RCC, Lima-
    Mendonça A, Vaníčková L, Mendonça AL, Do Nascimento RR. 2015. Identification of male-
    borne attractants in Anastrepha fraterculus (Diptera: Tephritidae). Chemoecology. 25 (3):
    115-122. DOI: 10.1007/s00049-014-0180-3
  • Vaníčková L, Virgilio M, Tomčala A, Břízová R, Ekesi S, Hoskovec M, Kalinová B, Do
    Nascimento RR, De Meyer, M. 2014. Resolution of three cryptic agricultural pests (Ceratitis
    fasciventris, C. anonae, C. rosa, Diptera: Tephritidae) using cuticular hydrocarbon profiling.
    Bulletin of Entomological Research 104(5): 631-638.
    DOI: https://doi.org/10.1017/S0007485314000406
  • Břízová R, Mendonça AL, Vaníčková L, Lima-Mendonça A, da Silva CE, Tomčala A,
    Paranhos BAJ, Dias VS, Joachim-Bravo IS, Hoskovec M and others. 2013. Pheromone
    analyses of the Anastrepha fraterculus (Diptera: Tephritidae) cryptic species complex. Florida
    Entomologist 96(3):1107-1115. DOI: 10.1653/024.096.0351
  • Gonçalves GB, Silva CE, Mendonça ADL, Vaníčková L, Tomčala A, Do Nascimento RR.
    2013. Pheromone Communication in Anastrepha obliqua (Diptera: Tephritidae): A
    comparison of the volatiles and salivary gland extracts of two wild populations. Florida
    Entomologist 96(4):1365-1374. DOI: https://doi.org/10.1653/024.096.0416
  • Abdulmanea K, Prokudina EA, Lanková P, Vaníčková L, Koblovská R, Zelený V, Lapčík O.
    2012. Immunochemical and HPLC identification of isoflavonoids in the Apiaceae family.
    Biochemical Systematics and Ecology 45:237-243. DOI:
    https://doi.org/10.1016/j.bse.2012.08.002
  • Vaníčková L, Do Nascimento RR, Hoskovec M, Ježková Z, Břízová R, Tomčala A, Kalinová
    B. 2012. Are the wild and laboratory insect populations different in semiochemical emission?
    The case of medfly sex pheromone. Journal of Agricultural and Food Chemistry
    60:7168−7176. doi: 10.1021/jf301474d
  • Vaníčková L, Svatoš A, Kroiss J, Kaltenpoth M, Do Nascimento RR, Hoskovec M, Břízová
    R, Kalinová B. 2012. Cuticular hydrocarbons of the South American fruit fly Anastrepha
    fraterculus: variability with sex and age. Journal of Chemical Ecology 38(9):1133-1142.
    DOI: 10.1007/s10886-012-0177-8
  • Abreu PM, Matthew S, González T, Vanickova L, Costa D, Gomes A, Segundo MA,
    Fernandes E. 2007. Isolation and identification of antioxidants from Pedilanthus tithymaloides.
    Journal of Natural Medicines 62(1):67-70. DOI: 10.1007/s11418-007-0186-z