Research group for bioanalysis and genetic engineering

A multidisciplinary research group is focused on a wide range of scientific topics covering plant biology, elemental analysis and bioanalysis. Newly developed or optimized methods are relevant not only to the field of conventional applications (environmental analysis, food analysis, medical diagnostics, etc.), but are capable to assess important biological context or to monitor external factors for key biological processes.

The use of sophisticated analytical instruments (spectrometric or separation techniques) as well as cutting-edge approaches of modern molecular biology (genome editing) creates a complex research strategy.

Výzkumná skupina bionanoanalýzy a genového inženýrství

One of the possible advantages of multidisciplinarity and multi-instrumentality is the ability to obtain information not only about metal content (AAS, (LA)-ICP-MS), but also about key biomolecules (DART / DESI-MS, GC-MS) including their spatial distribution.

The research group has a very good network for collaboration with international co-workers, but also for commercial activities and especially in the field of metals and their speciation.




doc. Mgr. Markéta Vaculovičová, Ph.D.

Head – Research group for bioanalysis and genetic engineering
Academic staff – Associate Professor

Phone: 420545 13 32 90
Address: ÚCB AF, Zemědělská 1, 61300 Brno – Building D
Office: BA02N3010
Researcher ID: E-5583-2016
ORCID: 0000-0002-6771-1304

Team members

Topics of Ph.D. theses

  • Volné téma-kovy v životním prostředí
  • Volné téma – oblast nanotechnologie v zemědělství
  • Volné téma – oblast nanočástice a rostliny
  • Biologická dostupnost rtuti pro užitkové plodiny
  • Volné téma
  • Volné téma – oblast nanomateriálů a mikrořas a biotechnologie
  • Volné téma – oblast genetického inženýrství rostlin/mikřas studium sekundárního metabolismu
  • Optional topic


  • AZV ČR: Role signalizace monocytárních buněk, jejich metabolických změn a transkripčních faktorů u pacientů se závažnou sepsí.. 2018-2021.
  • Evropská komise: Integrované systémy pro efektivní remediaci životního prostředí (GREENER). 2019-2022.
  • Evropská komise: Galileo Enhanced Solution for Pest Detection and Control in Greenhouse Fields with Autonomous Service Robots. 2017-2020.
  • FAO/IAEA: Kutikulární uhlovodíky: Nový chemotaxonomický a chemoekologický
    nástroj pro kontrolu zemědělských škůdců vrtulí z rodů Zeugodacus a Bactrocera. 2019-2023.
  • GAČR: Paperfluidická přenosná zařizení pro rychlou a nízkonákladovou analýzu bez instrumentální detekce. 2019-2021.


  • Ridoskova, A.; Pelfrene, A.; Douay, F.; Pelcova, P.; Smolikova, V.; Adam, V.; Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus. Environ. Toxicol. Chem. 2019, 38, 321-328. DOI: 10.1002/etc.4318.
  • Tvrdonova, M.; Vlcnovska, M.; Vanickova, L.; Kanicky, V.; Adam, V.; Ascher, L.; Vaculovicova, M.; Vaculovic, T.; Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Analytical and Bioanalytical Chemistry 2019, 411, 559-564. DOI: 10.1007/s00216-018-1300-7.
  • Rahbar, M.; Nesterenko, P. N.; Paull, B.; Macka, M.; High-throughput deposition of chemical reagents via pen-plotting technique for microfluidic paper-based analytical devices. Analytica Chimica Acta 2019, 1047, 115-123. DOI: 10.1016/j.aca.2018.09.006.
  • Islam, M. A.; Mahbub, P.; Nesterenko, P. N.; Paull, B.; Macka, M.; Prospects of pulsed amperometric detection in flow-based analytical systems – A review. Analytica Chimica Acta 2019, 1052, 10-26. DOI: 10.1016/j.aca.2018.10.066.
  • Rahbar, M.; Paull, B.; Macka, M.; Instrument-free argentometric determination of chloride via trapezoidal distance-based microfluidic paper devices. Analytica Chimica Acta 2019, 1063, 1-8. DOI: 10.1016/j.aca.2019.02.048.
  • Vaneckova, T.; Bezdekova, A.; Han, G.; Adam, V.; Vaculovicova, M.; Application of molecularly imprinted polymers as artificial receptors for imaging. Acta Biomater. 2019, in press, . DOI: 10.1016/j.actbio.2019.11.007.
  • Huang, L.; Kakadiaris; Vaneckova, T.; Huang, K.; Vaculovicova, M.; Han, G.; Designing Next Generation of Photon Upconversion: Recent advances in Organic Triplet-triplet Annihilation Upconversion Nanoparticles. Biomaterials 2019, 201, 77-86. DOI: 10.1016/j.biomaterials.2019.02.008.
  • Islam, M. A.; Lam, S. C.; Li, Y.; Atia, M. A.; Mahbub, P.; Nesterenko, P. N.; Paull, B.; Macka, M.; Capillary gap flow cell as capillary-end electrochemical detector in flow-based analysis. Electrochim. Acta 2019, 303, 85-93. DOI: 10.1016/j.electacta.2019.02.026.
  • Islam, M. A.; Atia, M. A.; Macka, M.; Paull, B.; Mahbub, P.; Electrochemical characterisation of nanoparticulate zirconium dioxide-on-gold electrode for electrochemical detection in flow-based analytical systems. Electrochim. Acta 2019, 318, 61-68. DOI: 10.1016/j.electacta.2019.06.031.
  • Islam, M. A.; Koreshkova, A. N.; Gupta, V.; Lewis, T.; Macka, M.; Paull, B.; Mahbub, P.; Fast pulsed amperometric waveform for miniaturised flow-through electrochemical detection: Application in monitoring graphene oxide reduction. Electrochim. Acta 2019, 328, 1-8. DOI: 10.1016/j.electacta.2019.135087.
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Pelcova, P.; Zouharova, I.; Ridoskova, A.; Smolikova, V.; Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model. Chemosphere 2019, 234, 373-378. DOI: 10.1016/j.chemosphere.2019.06.076.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Kolackova, M.; Moulick, A.; Kopel, P.; Dvorak, M.; Adam, V.; Klejdus, B.; Huska, D.; Antioxidant, gene expression and metabolomics fingerprint analysis of Arabidopsis thaliana treated by foliar spraying of ZnSe quantum dots and their growth inhibition of Agrobacterium tumefaciens. J. Hazard. Mater. 2019, 365, 932-941. DOI: 10.1016/j.jhazmat.2018.11.065.
  • Strejckova, A.; Dvorak, M.; Klejdus, B.; Krystofova, O.; Hedbavny, J.; Adam, V.; Huska, D.; The strong reaction of simple phenolic acids during oxidative stress caused by nickel, cadmium and copper in the microalga Scenedesmus quadricauda. New Biotech. 2019, 48, 66-75. DOI: 10.1016/j.nbt.2018.07.004.
  • Vaneckova, T.; Vanickova, L.; Tvrdonova, M.; Pomorski, A.; Krezel, A.; Vaculovic, T.; Kanicky, V.; Vaculovicova, M.; Adam, V.; Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing. Talanta 2019, 198, 224-229. DOI: 10.1016/j.talanta.2019.01.089.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Alves, M. N.; Miro, M.; Breadmore, M. C.; Macka, M.; Trends in analytical separations of magnetic (nano)particles. TRAC-Trends Anal. Chem. 2019, 114, 89-97. DOI: 10.1016/j.trac.2019.02.026.
  • Rahbar, M.; Wheeler, A. R.; Paull, B.; Macka, M.; Ion-exchange based immobilization of chromogenic reagents on microfluidic paper analytical devices. Analytical Chemistry 2019, 91, 8756-8761. DOI: 10.1021/acs.analchem.9b01288.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .