Research group for bioanalysis and genetic engineering

A multidisciplinary research group is focused on a wide range of scientific topics covering plant biology, elemental analysis and bioanalysis. Newly developed or optimized methods are relevant not only to the field of conventional applications (environmental analysis, food analysis, medical diagnostics, etc.), but are capable to assess important biological context or to monitor external factors for key biological processes.

The use of sophisticated analytical instruments (spectrometric or separation techniques) as well as cutting-edge approaches of modern molecular biology (genome editing) creates a complex research strategy.

One of the possible advantages of multidisciplinarity and multi-instrumentality is the ability to obtain information not only about metal content (AAS, (LA)-ICP-MS), but also about key biomolecules (DART / DESI-MS, GC-MS) including their spatial distribution.

The research group has a very good network for collaboration with international co-workers, but also for commercial activities and especially in the field of metals and their speciation.

doc. Mgr. Markéta Vaculovičová, Ph.D.

Head - Research group for bioanalysis and genetic engineering
Academic staff - Associate Professor

Phone: 420545 13 32 90
Address: ÚCB AF, Zemědělská 1, 61300 Brno - Budova D
Office: BA02N3010
Researcher ID: E-5583-2016
ORCID: 0000-0002-6771-1304

Team members

  • doc. Mgr. Markéta Vaculovičová, Ph.D.
  • Ing. Dalibor Húska, Ph.D.
  • Ing. Lukáš Nejdl, Ph.D.
  • Ing. Lucie Pompeiano Vaníčková, PhD.
  • Ing., Andrea Ridošková, Ph.D.
  • RNDr. Josef Hedbávný
  • Ing. Martina Koláčková, Ph.D.
  • Mgr. Olga Kryštofová, Ph.D.
  • prof. RNDr. Miroslav Macka, Ph.D.
  • M.Sc. Amitava Moulick, Ph.D.
  • Ing. Helena Novotná, Ph.D.
  • doc. Mgr. Pavlína Pelcová, Ph.D.
  • Mgr. Dagmar Štěrbová, Ph.D.
  • Ing. Libor Lenža
  • Ing. Jaroslava Bezděková
  • MSc. Sukanya Bhowmick
  • Ing. Markéta Dobešová
  • Mgr. Marek Dvořák
  • Ing. Pavel Chaloupský
  • Ing. Anna Jánová
  • M.Sc. Aninda Mazumdar
  • Ing. Kristýna Pavelicová
  • Ing. Ivan Rankic
  • Ing. Vendula Smolíková
  • M.Sc. Vishma Pratap Sur
  • Ing. Vladimíra Tarbajová
  • Ing. Radim Zelinka
  • Ing. Kristýna Zemánková

Topics of Ph.D. theses

  • Optional topic
  • Biologická dostupnost rtuti pro užitkové plodiny
  • Volné téma
  • Volné téma-kovy v životním prostředí
  • Volné téma - oblast nanotechnologie v zemědělství
  • Volné téma - oblast nanomateriálů a mikrořas a biotechnologie
  • Volné téma - oblast genetického inženýrství rostlin/mikřas studium sekundárního metabolismu
  • Volné téma - oblast nanočástice a rostliny


  • AZV ČR: Role of monocyte metabolism and transcription factor networks in progression of human sepsis.. 2018-2021.
  • Evropská komise: InteGRated systems for Effective ENvironmEntal Remediation. 2019-2022.
  • Evropská komise: Galileo Enhanced Solution for Pest Detection and Control in Greenhouse Fields with Autonomous Service Robots. 2017-2020.
  • FAO/IAEA: Assessment of Simultaneous Application of SIT and MAT to Enhance Bactrocera Fruit Fly Management. 2019-2023.
  • GAČR: Paperfluidic-based rapid low cost portable analytical devices with instrumentation-free readout. 2019-2021.


  • Ridoskova, A.; Pelfrene, A.; Douay, F.; Pelcova, P.; Smolikova, V.; Adam, V.; Bioavailability of mercury in contaminated soils assessed by the diffusive gradient in thin film technique in relation to uptake by Miscanthus × giganteus. Environ. Toxicol. Chem. 2019, 38, 321-328. DOI: 10.1002/etc.4318.
  • Tvrdonova, M.; Vlcnovska, M.; Vanickova, L.; Kanicky, V.; Adam, V.; Ascher, L.; Vaculovicova, M.; Vaculovic, T.; Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Analytical and Bioanalytical Chemistry 2019, 411, 559-564. DOI: 10.1007/s00216-018-1300-7.
  • Rahbar, M.; Nesterenko, P. N.; Paull, B.; Macka, M.; High-throughput deposition of chemical reagents via pen-plotting technique for microfluidic paper-based analytical devices. Analytica Chimica Acta 2019, 1047, 115-123. DOI: 10.1016/j.aca.2018.09.006.
  • Islam, M. A.; Mahbub, P.; Nesterenko, P. N.; Paull, B.; Macka, M.; Prospects of pulsed amperometric detection in flow-based analytical systems - A review. Analytica Chimica Acta 2019, 1052, 10-26. DOI: 10.1016/j.aca.2018.10.066.
  • Rahbar, M.; Paull, B.; Macka, M.; Instrument-free argentometric determination of chloride via trapezoidal distance-based microfluidic paper devices. Analytica Chimica Acta 2019, 1063, 1-8. DOI: 10.1016/j.aca.2019.02.048.
  • Vaneckova, T.; Bezdekova, A.; Han, G.; Adam, V.; Vaculovicova, M.; Application of molecularly imprinted polymers as artificial receptors for imaging. Acta Biomater. 2019, in press, . DOI: 10.1016/j.actbio.2019.11.007.
  • Huang, L.; Kakadiaris; Vaneckova, T.; Huang, K.; Vaculovicova, M.; Han, G.; Designing Next Generation of Photon Upconversion: Recent advances in Organic Triplet-triplet Annihilation Upconversion Nanoparticles. Biomaterials 2019, 201, 77-86. DOI: 10.1016/j.biomaterials.2019.02.008.
  • Islam, M. A.; Lam, S. C.; Li, Y.; Atia, M. A.; Mahbub, P.; Nesterenko, P. N.; Paull, B.; Macka, M.; Capillary gap flow cell as capillary-end electrochemical detector in flow-based analysis. Electrochim. Acta 2019, 303, 85-93. DOI: 10.1016/j.electacta.2019.02.026.
  • Islam, M. A.; Atia, M. A.; Macka, M.; Paull, B.; Mahbub, P.; Electrochemical characterisation of nanoparticulate zirconium dioxide-on-gold electrode for electrochemical detection in flow-based analytical systems. Electrochim. Acta 2019, 318, 61-68. DOI: 10.1016/j.electacta.2019.06.031.
  • Islam, M. A.; Koreshkova, A. N.; Gupta, V.; Lewis, T.; Macka, M.; Paull, B.; Mahbub, P.; Fast pulsed amperometric waveform for miniaturised flow-through electrochemical detection: Application in monitoring graphene oxide reduction. Electrochim. Acta 2019, 328, 1-8. DOI: 10.1016/j.electacta.2019.135087.
  • Brtnicky, M.; Pecina, V.; Hladky, J.; Radziemska, M.; Koudelkova, Z.; Klimanek, M.; Richtera, L.; Adamcova, D.; Elbl, J.; Galiova, M. V.; Balakova, L.; Kynicky, J.; Smolikova, V.; Houska, J.; Vaverkova, M. D.; Assessment of phytotoxicity, environmental and health risks of historical urban park soils. Chemosphere 2019, 220, 678-686. DOI: 10.1016/j.chemosphere.2018.12.188.
  • Pelcova, P.; Zouharova, I.; Ridoskova, A.; Smolikova, V.; Evaluation of mercury availability to pea parts (Pisum sativum L.) in urban soils: Comparison between diffusive gradients in thin films technique and plant model. Chemosphere 2019, 234, 373-378. DOI: 10.1016/j.chemosphere.2019.06.076.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Kolackova, M.; Moulick, A.; Kopel, P.; Dvorak, M.; Adam, V.; Klejdus, B.; Huska, D.; Antioxidant, gene expression and metabolomics fingerprint analysis of Arabidopsis thaliana treated by foliar spraying of ZnSe quantum dots and their growth inhibition of Agrobacterium tumefaciens. J. Hazard. Mater. 2019, 365, 932-941. DOI: 10.1016/j.jhazmat.2018.11.065.
  • Strejckova, A.; Dvorak, M.; Klejdus, B.; Krystofova, O.; Hedbavny, J.; Adam, V.; Huska, D.; The strong reaction of simple phenolic acids during oxidative stress caused by nickel, cadmium and copper in the microalga Scenedesmus quadricauda. New Biotech. 2019, 48, 66-75. DOI: 10.1016/j.nbt.2018.07.004.
  • Vaneckova, T.; Vanickova, L.; Tvrdonova, M.; Pomorski, A.; Krezel, A.; Vaculovic, T.; Kanicky, V.; Vaculovicova, M.; Adam, V.; Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing. Talanta 2019, 198, 224-229. DOI: 10.1016/j.talanta.2019.01.089.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Alves, M. N.; Miro, M.; Breadmore, M. C.; Macka, M.; Trends in analytical separations of magnetic (nano)particles. TRAC-Trends Anal. Chem. 2019, 114, 89-97. DOI: 10.1016/j.trac.2019.02.026.
  • Rahbar, M.; Wheeler, A. R.; Paull, B.; Macka, M.; Ion-exchange based immobilization of chromogenic reagents on microfluidic paper analytical devices. Analytical Chemistry 2019, 91, 8756-8761. DOI: 10.1021/acs.analchem.9b01288.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .