Laboratory of plant metabolomics and epigenetics

The main vision of the laboratory is to master genetic engineering techniques, especially the Crispr/Cas9 and CRISPRi systems for regulation of gene expression. Taking into account the extreme weather changes and future food sources, the laboratory investigates how small RNAs (miRNAs, siRNAs) and other epigenetic mechanisms affect the biosynthesis of secondary metabolites.

Another research area is unicellular algae, which belong to organisms with a large source of genetic and metabolic diversity. Research on biologically active substances and their relationship to microalgae metabolism is currently underway, and the possibilities of metabolic engineering are being examined. New methods of microalgae cultivation and extraction of these compounds are also being sought. The research also focuses on the study of nanoparticles potentially useful in the protection of agricultural crops against abiotic and biotic stress factors and in the stimulation of production of biologically active substances.





Ing. Dalibor Húska, Ph.D.

Head - Laboratory of plant metabolomics and epigenetics
Assistant Professor


Phone: 420 545 133 280
Address: ÚCB AF, Zemědělská 1, 61300 Brno - Budova D
Office: BA02N2028
E-mail: dalibor.huska@mendelu.cz

Team members

  • Ing. Dalibor Húska, Ph.D.
  • Ing. Martina Koláčková, Ph.D.
  • Mgr. Olga Kryštofová, Ph.D.
  • M.Sc. Amitava Moulick, Ph.D.
  • Ing. Helena Novotná, Ph.D.
  • Mgr. Dagmar Štěrbová, Ph.D.
  • Ing. Libor Lenža
  • MSc. Sukanya Bhowmick
  • Ing. Markéta Dobešová
  • Mgr. Marek Dvořák
  • Ing. Pavel Chaloupský
  • Ing. Anna Jánová
  • M.Sc. Aninda Mazumdar
  • Ing. Ivan Rankic
  • M.Sc. Vishma Pratap Sur
  • Ing. Vladimíra Tarbajová
  • Ing. Radim Zelinka

Topics of Ph.D. theses

  • Volné téma - oblast genetického inženýrství rostlin/mikřas studium sekundárního metabolismu
  • Volné téma - oblast nanotechnologie v zemědělství
  • Optional topic
  • Volné téma - oblast nanomateriálů a mikrořas a biotechnologie
  • Volné téma - oblast nanočástice a rostliny


Projects

  • Mendelu: Utilization of nanocomposite materials to eliminate the impact of drought stress and excessive irradiation in maize seed crops. 2019-2020.


Publications

  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Kolackova, M.; Moulick, A.; Kopel, P.; Dvorak, M.; Adam, V.; Klejdus, B.; Huska, D.; Antioxidant, gene expression and metabolomics fingerprint analysis of Arabidopsis thaliana treated by foliar spraying of ZnSe quantum dots and their growth inhibition of Agrobacterium tumefaciens. J. Hazard. Mater. 2019, 365, 932-941. DOI: 10.1016/j.jhazmat.2018.11.065.
  • Strejckova, A.; Dvorak, M.; Klejdus, B.; Krystofova, O.; Hedbavny, J.; Adam, V.; Huska, D.; The strong reaction of simple phenolic acids during oxidative stress caused by nickel, cadmium and copper in the microalga Scenedesmus quadricauda. New Biotech. 2019, 48, 66-75. DOI: 10.1016/j.nbt.2018.07.004.
  • Jimenez Jimenez, A. M.; Moulick, A.; Bhowmick, S.; Strmiska, V.; Gagic, M.; Horakova, Z.; Kostrica, R.; Masarik, M.; Heger, Z.; Adam, V.; One-step detection of human papilloma viral infection using quantum dot-nucleotide interaction specificity. Talanta 2019, 205, 1-8. DOI: 10.1016/j.talanta.2019.07.006.
  • Milosavljevic, V.; Jamroz, E.; Gagic, M.; Haddad, Y.; Michalkova, H.; Balkova, R.; Tesarova, B.; Moulick, A.; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery. Biomacromolecules 2019, in press, . DOI: 10.1021/acs.biomac.9b01175 .
  • Vaneckova, T.; Bezdekova, J.; Tvrdonova, M.; Vlcnovska, M.; Novotna, V.; Neuman, J.; Stossova, A.; Kanicky, V.; Adam, V.; Vaculovicova, M.; Vaculovic, T.; CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. Sci. Rep. 2019, 9, 1-9. DOI: 10.1038/s41598-019-48290-2.
  • Bacova, R.; Klejdus, B.; Ryant, P.; Cernei, N.; Adam, V.; Huska, D.; The effects of 5-azacytidine and cadmium on global 5-methylcytosine content and secondary metabolites in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus quadricauda. J. Phycol. 2019, 55, 329-342. DOI: 10.1111/jpy.12819.
  • Gutierrez; Ansuategi; Susperregi,; Tub; Ranki; Lenza, L.; A benchmarking of learning strategies for pest detection and identification on tomato plants for autonomous scouting robots using internal databases. J. Sens. 2019, 2019, 1-15. DOI: 10.1155/2019/5219471.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.; Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Vsiansky; Svobodova; Gumulec; Cernei, N.; Sterbova, D.; Zitka, O.; Kostrica, R.; Smilek; Plzak; Betka; Kalfert; Masarik, M.; Raudenska, M.; Prognostic significance of serum free amino acids in head and neck cancers. Cells 2019, 8, 1-12. DOI: 10.3390/cells8050428.
  • Sur, V. P.; Kominkova, M.; Buchtova, Z.; Dolezelikova, K.; Zitka, O.; Moulick, A.; CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing. Nanomaterials 2019, 9, 1-15. DOI: 10.3390/nano9101463.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Sturikova, H.; Krystofova, O.; Huska, D.; Adam, V.; Zinc, zinc nanoparticles and plants. J. Hazard. Mater. 2018, 349, 101-110. DOI: 10.1016/j.jhazmat.2018.01.040.
  • Hynstova, V.; Sterbova, D.; Klejdus, B.; Hedbavny, J.; Huska, D.; Adam, V.; Separation, identification and quantification of carotenoids and chlorophylls in dietary supplements containing Chlorella vulgaris and Spirulina platensis using High Performance Thin Layer Chromatography. J. Pharm. Biomed. Anal. 2018, 148, 108-118. DOI: 10.1016/j.jpba.2017.09.018.
  • Jimenez Jimenez, A. M.; Moulick, A.; Richtera, L.; Krejcova, L.; Kalina, L.; Datta, R.; Svobodova, M.; Hynek, D.; Masarik, M.; Heger, Z.; Adam, V.; Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection. Sens. Actuator B-Chem. 2018, 258, 295-303. DOI: 10.1016/j.snb.2017.11.074.
  • Milosavljevic, V.; Haddad, Y.; Moulick, A.; Buchtelova, H.; Guran, R.; Pospisil, T.; Stokowa-So; Heger, Z.; Richtera, L.; Kopel, P.; Adam, V.; Functional analysis of novicidin peptide: Coordinated delivery system for zinc via Schiff base ligand. Bioconjugate Chem. 2018, 29, 2954. DOI: 10.1021/acs.bioconjchem.8b00370.
  • Moulick, A.; Heger, Z.; Milosavljevic, V.; Richtera, L.; Barroso; Merlos, M. A. R.; Buchtelova, H.; Podgajny, R.; Hynek, D.; Kopel, P.; Adam, V.; Real-time visualization of cell membrane damage using gadolinium Schiff base complex-doped quantum dots. ACS Appl. Mater. Interfaces 2018, 10, 35859-35868. DOI: 10.1021/acsami.8b15868.
  • Dostalova, S.; Polanska, H.; Svobodova, M.; Balvan, J.; Krystofova, O.; Haddad, Y.; Krizkova, S.; Masarik, M.; Eckschlager, T.; Stiborova, M.; Heger, Z.; Adam, V.; Prostate-specific membrane antigen-targeted site-directed antibody-conjugated apoferritin nanovehicle favorably influences in vivo side effects of doxorubicin . Sci. Rep. 2018, 8, 1-13. DOI: 10.1038/s41598-018-26772-z.
  • Buchtelova, H.; Strmiska, V.; Skubalova, Z.; Dostalova, S.; Michalek, P.; Krizkova, S.; Hynek, D.; Kalina, L.; Richtera, L.; Moulick, A.; Adam, V.; Heger, Z.; Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping. J. Nanobiotechnol. 2018, 16, 1-14. DOI: 10.1186/s12951-018-0369-7.
  • Jelinkova, P.; Splichal, Z.; Jimenez Jimenez, A. M.; Haddad, Y.; Mazumdar, A.; Sur, V. P.; Milosavljevic, V.; Kopel, P.; Buchtelova, H.; Guran, R.; Zitka, O.; Richtera, L.; Hegerova, D.; Heger, Z.; Moulick, A.; Adam, V.; Novel vancomycin–peptide conjugate as potent antibacterial agent against vancomycin-resistant Staphylococcus aureus. Infect. Drug Resistance 2018, 2018, 1807. DOI: 10.2147/IDR.S160975.