Laboratory of microbiology

The special focus of the Laboratory of microbiology resides in clinical, molecular and environmental microbiology. Particular emphasis is on the studies of new types of antibacterial materials based upon nanomaterials, peptides, proteins, coordinating compounds and composites.

These materials serve as alternatives to conventional antibiotics or, the other way round, they improve the efficiency of antibiotics against resistant bacterial pathogens. Understanding the mechanism of antibiotic resistance on the molecular level and overcoming the same by new types of materials, as well as targeting these materials upon pathogens without affecting the surrounding microbiome, is yet another research objective of the lab. Last but not least, the investigation aims at the identification of microorganisms from clinical and veterinary samples. The obtained microbial cultures are subject to genotyping and characterization as to their antibiotic resistance and stored in the laboratory collection of bacterial cultures. 

Another important research orientation is the production and characterization of biologically significant enzymes for the purpose of immobilization, as well as studying their environmental application, including bioremediation of contaminated soils, water or different kinds of waste.  

The laboratory is equipped with the bacterial growth analyzers, an automatic testing device determining the sensitivity to antibiotics, advanced incubators for the cultivation of microorganisms, various microscopic techniques, a flow cytometer and devices for molecular biological techniques (PCR, sequencing, cloning).





Ing. Kristýna Šmerková, PhD.

Head - Laboratory of microbiology
Assistant Professor


Phone: 420 545 133 293
Address: ÚCB AF, Zemědělská 1, 61300 Brno - Budova D
Office: BA02N3011
E-mail: kristyna.smerkova@mendelu.cz

Team members

  • Ing. Kristýna Šmerková, PhD.
  • Hanadi Abd Al Rahman Ali Ananbeh, MSc., Ph.D.
  • Mgr. Lucie Bozděchová, Ph.D.
  • Ing. Kristýna Doleželíková, Ph.D.
  • prof. Ing. Luděk Žůrek, Ph.D.
  • M.Sc. Bellah Younis AlMotasem
  • Ing. Tatiana Fialová
  • Ing. Silvia Kočiová
  • Ing. Ludmila Košarišťanová
  • Mgr. Michaela Kuthanová

Topics of Ph.D. theses

  • Volné téma - imobilizace enzymů
  • Mechanismus vzniku bakteriální resistence k antimikrobiálním nanomateriálům
  • Nanomateriály a nové typy antimikrobiálních látek jako alternativa k antibiotickým preparátům
  • Optional topic
  • Volné téma - v případě zájmu kontaktujte vedoucího výzkumné skupiny
  • Vliv kovových a polokovových nanomateriálů na vznik bakteriální resistence
  • Studium mikrobiomu u zdravých a mastitidních krav


Projects

  • Mendelu: Effect of combination of polyunsaturated fatty acids and nanoparticles on cutaneous wound healing in a model organism. 2019-2020.
  • MŠMT: . 2018-2022.
  • TAČR: Gastro-processing waste into a solid carbon product for material use. 2019-2021.


Publications

  • Bytesnikova, Z.; Richtera, L.; Smerkova, K.; Adam, V.; The graphene oxide as a tool for antibiotic resistance genes removal: A review. Environ. Sci. Pollut. Res. 2019, 26, 20148-20163. DOI: 10.1007/s11356-019-05283-y.
  • Jelinkova, P.; Mazumdar, A.; Sur, V. P.; Kociova, S.; Dolezelikova, K.; Jimenez Jimenez, A. M.; Koudelkova, Z.; Mishra, P. K.; Smerkova, K.; Heger, Z.; Vaculovicova, M.; Moulick, A.; Adam, V.; Nanoparticle-drug conjugates treating bacterial infections. J. Control. Release 2019, 307, 166-185. DOI: 10.1016/j.jconrel.2019.06.013.
  • Buchtelova, H.; Skubalova, Z.; Strmiska, V.; Michalek, P.; Kociova, S.; Smerkova, K.; Kruszynski,; Bienko,; Kaj,; Lewinska,; Bienko,; Malik-Gajewska,; Milosavljevic,; Kopel,; Heger,; Adam, V.; Synthesis and structural characterization of antimicrobial binuclear copper (II) coordination compounds bridged by hydroxy- and/or thiodipropionic acid. J. Inorg. Biochem. 2019, 191, 8-20. DOI: 10.1016/j.jinorgbio.2018.10.011.
  • Ananbeh, H. A. A. A.; Stojanovic, M.; Pompeiano, A.; Voberkova, S.; Trasar-Cepeda, C.; Use of soil enzyme activities to assess the recovery of soil functions in abandoned coppice forest systems. Sci. Total Environ. 2019, in press, . DOI: 10.1016/j.scitotenv.2019.133692.
  • Horky, P.; Skalickova, S.; Urbankova, L.; Baholet, D.; Kociova, S.; Bytesnikova, Z.; Kabourkova, E.; Lackova, Z.; Cernei, N.; Gagic, M.; Milosavljevic, V.; Smolikova, V.; Vaclavkova, E.; Nevrkla, P.; Knot, P.; Krystofova, O.; Hynek, D.; Kopel, P.; Skladanka, J.; Adam, V.; Smerkova, K.; Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. J. Anim. Sci. Biotechnol. 2019, 10, 1-12. DOI: 10.1186/s40104-019-0319-8.
  • Horky, P.; Skalickova, S.; Smerkova, K.; Skladanka, J.; Essential oils as a feed additives: Pharmacokinetics and potential toxicity in monogastric animals. Animals 2019, 9, 1-15. DOI: 10.3390/ani9060352.
  • Sur, V. P.; Kominkova, M.; Buchtova, Z.; Dolezelikova, K.; Zitka, O.; Moulick, A.; CdSe QD biosynthesis in yeast using tryptone-enriched media and their conjugation with a peptide hecate for bacterial detection and killing. Nanomaterials 2019, 9, 1-15. DOI: 10.3390/nano9101463.
  • Vaneckova, T.; Smerkova, K.; Zitka, J.; Hynek, D.; Zitka, O.; Hlavacek, A.; Foret, F.; Adam, V.; Vaculovicova, M.; Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis. Electrophoresis 2018, 39, 2246-2252. DOI: 10.1002/elps.201700483.
  • Nejdl, L.; Moravanska, A.; Smerkova, K.; Mravec, F.; Krizkova, S.; Pomorski, A.; Krezel, A.; Macka, M.; Adam, V.; Vaculovicova, M.; Short-sweep capillary electrophoresis with a selective zinc fluorescence imaging reagent FluoZin-3 for determination of free and metalothionein-2a-bound Zn2+ ions. Analytica Chimica Acta 2018, 1017, 41-47. DOI: 10.1016/j.aca.2018.02.008.
  • Jelinkova, P.; Vesely, R.; Cihalova, K.; Hegerova, D.; Ananbeh, H. A. A. A.; Richtera, L.; Smerkova, K.; Brtnicky, M.; Kynicky, J.; Moulick, A.; Adam, V.; Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus. Environ. Res. 2018, 166, 394-401. DOI: 10.1016/j.envres.2018.06.024.
  • Smerkova, K.; Vaculovic, T.; Vaculovicova, M.; Kynicky, J.; Brtnicky, M.; Eckschlager, T.; Stiborova, M.; Hubalek, J.; Adam, V.; DNA interaction with platinum-based cytostatics revealed by DNA sequencing. Anal. Biochem. 2017, 539, 22-28. DOI: 10.1016/j.ab.2017.09.018.
  • Nejdl, L.; Kudr, J.; Moulick, A.; Hegerova, D.; Ruttkay-Nedecky, B.; Gumulec, J.; Cihalova, K.; Smerkova, K.; Dostalova, S.; Krizkova, S.; Novotna, K.; Kopel, P.; Adam, V.; Platinum nanoparticles induce damage to DNA and inhibit DNA replication. PLoS ONE 2017, 12, 1-19. DOI: 10.1371/journal.pone.0180798.
  • Blazkova, I.; Smerkova, K.; Blazkova, L.; Vaculovicova, M.; Stiborova, M.; Eckschlager, T.; Beklova, M.; Adam, V.; Kizek, R.; Doxorubicin interactions with bovine serum albumin revealed by microdialysis with on-line laser-induced fluorescence detection at sub-picogram level. Electrophoresis 2015, 36, 1282. DOI: 10.1002/elps.201400564.
  • Chudobova, D.; Cihalova, K.; Guran, R.; Dostalova, S.; Smerkova, K.; Vesely, R.; Gumulec, J.; Masarik, M.; Heger, Z.; Adam, V.; Kizek, R.; Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration. Braz. J. Infect. Dis. 2015, 19, 604. DOI: 10.1016/j.bjid.2015.08.013.
  • Kudr, J.; Nejdl, L.; Skalickova, S.; Zurek, M.; Milosavljevic, V.; Kensova, R.; Ruttkay-Nedecky, B.; Kopel, P.; Hynek, D.; Novotna, M.; Adam, V.; Kizek, R.; Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles. J. Mat. Chem. B 2015, 3, 2109-2118. DOI: 10.1039/c4tb01336k.
  • Kominkova, M.; Michalek, P.; Cihalova, K.; Guran, R.; Cernei, N.; Nejdl, L.; Smerkova, K.; Dostalova, S.; Chudobova, D.; Heger, Z.; Vesely, R.; Gumulec, J.; Kynicky, J.; Xhaxhiu; Zitka, O.; Adam, V.; KIzek, R.; Study of linkage between glutathione pathway and the antibiotic resistance of Escherichia coli from patients’ swabs. Int. J. Mol. Sci. 2015, 16, 7210-7229. DOI: 10.3390/ijms16047210.
  • Smerkova, K.; Hudcova, K.; Vlahova, V.; Vaculovicova, M.; Pekarik, V.; Masarik, M.; Adam, V.; Kizek, R.; Label-free and amplification-free miR-124 detection in human cells. Int. J. Oncol. 2015, 46, 871-877. DOI: 10.3892/ijo.2014.2756.
  • Smerkova, K.; Vlcnovska, M.; Dostalova, S.; Milosavljevic, V.; Kopel, P.; Vaculovic, T.; Krizkova, S.; Vaculovicova, M.; Adam, V.; Kizek, R.; ELISA-like analysis of cisplatinated DNA using magnetic separation. Nanobiomedicine 2015, 2, 1-8. DOI: 10.5772/61699 .
  • Nejdl, L.; Kudr, J.; Cihalova, K.; Chudobova, D.; Zurek, M.; Zalud, L.; Kopecny, L.; Burian, F.; Ruttkay-Nedecky, B.; Krizkova, S.; Konecna, M.; Hynek, D.; Kopel, P.; Prasek, J.; Adam, V.; Kizek, R.; Remote-controlled robotic platform Orpheus as a new tool for detection of bacteria in the environment. Electrophoresis 2014, 35, 2333-2345. DOI: 10.1002/elps.201300576.
  • Krejcova, L.; Nejdl, L.; Merlos, M.A.R.; Zurek, M.; Matousek, M.; Hynek, D.; Zitka, O.; Kopel, P.; Adam, V.; Kizek, R.; 3D printed chip for electrochemical detection of influenza virus labeled with CdS quantum dots. Biosens. Bioelectron. 2014, 54, 421-427. DOI: 10.1016/j.bios.2013.10.031.