Laboratoř bioanalýzy a zobrazování

@article{pmid35101791,

title = {Detection of pesticides in food products using paper-based devices by UV-induced fluorescence spectroscopy combined with molecularly imprinted polymers},

author = {Milada Vodova and Lukas Nejdl and Kristyna Pavelicova and Kristyna Zemankova and Tomas Rrypar and Dagmar Skopalova Sterbova and Jaroslava Bezdekova and Nantana Nuchtavorn and Mirek Macka and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1016/j.foodchem.2022.132141},

issn = {1873-7072},

year  = {2022},

date = {2022-06-01},

journal = {Food Chem},

volume = {380},

pages = {132141},

abstract = {In this proof-of-concept study, we explore the detection of pesticides in food using a combined power of sensitive UV-induced fingerprint spectroscopy with selective capture by molecularly imprinted polymers (MIPs) and portable cost-effective paper-based analytical devices (PADs). The specific pesticides used herein as model compounds (both pure substances and their application products for spraying), were: strobilurins (i.e. trifloxystrobin), urea pesticides (rimsulfuron), pyrethroids (cypermethrine) and aryloxyphenoxyproponic acid herbicides (Haloxyfop-methyl). Commercially available spraying formulations containing the selected pesticides were positively identified by MIP-PADs swabs of sprayed apple and tomato. The key properties of MIP layer - imprinting factor (IF) and selectivity factor (α) were characterized using trifloxystrobin (IF-3.5, α-4.4) was demonstrated as a potential option for in-field application. The presented method may provide effective help with in-field testing of food and reveal problems such as false product labelling.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Mapping of melim melanoma combining icp-ms and maldi-msi methods.},

author = {VANÍČKOVÁ, L. and DO, T. and VEJVODOVÁ, M. and HORÁK, V. and HUBÁLEK, M. and EMRI, G. and ZEMÁNKOVÁ, K., and PAVELICOVÁ K. and KŘÍŽKOVÁ, S. and FALTUSOVÁ, V. and POMPEIANO, A. and VACULOVIČOVÁ, M. and ZÍTKA, O. and VACULOVIČ, T. and ADAM, V.},

url = {https://doi.org/10.1016/j.ijbiomac.2022.01.139},

year  = {2022},

date = {2022-05-11},

urldate = {2022-05-11},

journal = {International Journal of Biological Macromolecules},

volume = {vol. 203.},

pages = {583-592.},

abstract = {Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Distance-based detection in analytical flow devices: From gas detection tubes to microfluidic chips and microfluidic paper-based analytical devices},

author = {NUCHTAVORN, N. , and RYPAR, T., and NEJDL, L., and VACULOVICOVA, M., and MACKA, M.},

url = {https://doi.org/10.1016/j.trac.2022.116581},

issn = {0165-993},

year  = {2022},

date = {2022-03-01},

urldate = {2022-03-01},

journal = {TrAC – Trends in Analytical Chemistry},

volume = {150},

abstract = {Distance-based detection (DbD) in analytical flow devices was first described in the 1930s, based on a colorimetric measurement of gas samples passing through a tube with a solid porous packing showing a color change as it reacts with the gas, with the zone length proportional to the analyte concentration. Over the following decades, DbD was introduced to a variety of formats and platforms including capillary, microfluidic chips and microfluidic paper-based analytical devices. Most of the materials are easily functionalised, and disposable. The DbD principles are based on a visible color change resulting from various reactions ranging from simple colorimetric to specific ligand or antibody binding. The immense attractivity of DbD rests in its simplicity of quantitative readout without auxiliary instrumentation. This review outlines key historical developments during 1937–2021, detection principles, driving forces, instrumental formats, and application areas. Finally, ways to overcome limitations, improve the performance and future perspectives are discussed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Uv-light-actuated in-situ preparation of paper@zncd quantum dots for paper-based enzymatic nanoreactors.},

author = {KHUNKITCHAI, N. and NUCHTAVORN, N. and RYPAR, T. and VLCNOVSKA, M. and NEJDL, L. and VACULOVICOVA, M. and MACKA, M.},

url = {https://doi.org/10.1016/j.cej.2021.132508},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Chemical Engineering Journal.},

volume = {vol. 428.},

pages = {132508.},

abstract = {Quantum dots (QDs) have been widely applied in the analytical field including sensitive fluorescent assays on paper-based devices. A facile in-situ synthesis of QDs after a simple reagent deposition on paper could enable a rapid low-cost fabrication of QD–modified paper with a variety of properties and purposes such as enzymatic nanoreactors. Herein, for the first time, fluorescent ZnCd QDs were prepared by in-situ synthesis in the paper matrix using thiol-containing precursors and irradiation by UV light. A successful creation of the immobilized ZnCd QDs on paper (paper@ZnCd QDs) was monitored by their intrinsic fluorescence while their peroxidase mimetic activity was evaluated by a catalytic reaction between H2O2 and a substrate (3,3′,5,5′-tetramethylbenzidine, TMB) producing a blue coloured product of charged transfer complex of TMB (oxTMB). From three thiol precursors investigated, the insulin precursor provided a greater activity than glutathione (GSH) and both considerably larger than BSA. Finally, a QDs precursor mixture deposition onto the paper matrix was evaluated by the peroxidase-mimetic activity, which was comparable with a reference method. The in-situ preparation of paper@ZnCd QDs is simple, rapid and ‘green’, with potential in biomedical applications primarily as fluorescence imaging agent and enzyme mimetic paper-based nanoreactors.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid34496333,

title = {UV-Induced fingerprint spectroscopy},

author = {Lukas Nejdl and Martina Havlikova and Filip Mravec and Tomas Vaculovic and Veronika Faltusova and Kristyna Pavelicova and Mojmir Baron and Michal Kumsta and Vit Ondrousek and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1016/j.foodchem.2021.130499},

issn = {1873-7072},

year  = {2022},

date = {2022-01-01},

journal = {Food Chem},

volume = {368},

pages = {130499},

abstract = {Here, we present the potential analytical applications of photochemistry in combination with fluorescence fingerprinting. Our approach analyzes the fluorescence of samples after ultraviolet light (UV) treatment. Especially in presence of metal ions and thiol-containing compounds, the fluorescence behavior changes considerably. The UV-induced reactions (changes) are unique to a given sample composition, resulting in distinct patterns or fingerprints (typically in the 230-600 nm spectral region). This method works without the need for additional chemicals or fluorescent probes, only suitable diluent must be used. The proposed method (UV fingerprinting) suggests the option of recognizing various types of pharmaceuticals, beverages (juices and wines), and other samples within only a few minutes. In some studied samples (e.g. pharmaceuticals), significant changes in fluorescence characteristics (mainly fluorescence intensity) were observed. We believe that the fingerprinting technique can provide an innovative solution for analytical detection.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers.},

author = {Rypar, T., and Adam, V., and Vaculovicova, M. and & Macka, M. },

url = {https://doi.org/10.1016/j.snb.2021.129999},

year  = {2021},

date = {2021-04-19},

journal = {Sensors and Actuators B: Chemical},

volume = {341},

abstract = {Microfluidic paper-based analytical devices (μPADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When μPADs are combined with distance-based detection in DμPADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of DμPADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified DμPADs (MIP-DμPADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a DμPAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the DμPAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-DμPAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4–29.2 μM (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 μM and LOQ =11.8 μM. The here presented analytical concept based on MIP-DμPADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid33340626,

title = {Metallothionein dimerization evidenced by QD-based Förster resonance energy transfer and capillary electrophoresis},

author = {Kristyna Pavelicova and Lucie Vanickova and Yazan Haddad and Lukas Nejdl and Jan Zitka and Silvia Kociova and Filip Mravec and Tomas Vaculovic and Mirek Macka and Marketa Vaculovicova and Vojtech Adam},

doi = {10.1016/j.ijbiomac.2020.12.105},

issn = {1879-0003},

year  = {2021},

date = {2021-02-01},

journal = {Int J Biol Macromol},

volume = {170},

pages = {53--60},

abstract = {Herein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) and capillary electrophoresis with LED-induced detection. The FRET pair consisted of quantum dots (QDs) used as an emission tunable donor (emission wavelength of 450 nm) and a cyanine dye (Cy3), providing optimal optical properties as an acceptor. Nonoxidative dimerization of mammalian metallothionein (MT) was investigated using the donor and acceptor covalently conjugated to MT. The main functions of MTs within an organism include the transport and storage of essential metal ions and detoxification of toxic ions. Upon storage under aerobic conditions, MTs form dimers (as well as higher oligomers), which may play an essential role as mediators in oxidoreduction signaling pathways. Due to metal bridging by Cd ions between molecules of metallothionein, the QDs and Cy3 were close enough, enabling a FRET signal. The FRET efficiency was calculated to be in the range of 11-77%. The formation of MT dimers in the presence of Cd ions was confirmed by MALDI-MS analyses. Finally, the process of oligomerization resulting in FRET was monitored by CE, and oligomerization of MT was confirmed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Micellar electrokinetic chromatography as a powerful analytical tool for research on prebiotic chemistry.},

author = {ZEMANKOVA, K. and NEJDL, L. and BEZDEKOVA, J. and VODOVA, M. and PETERA, L. and PASTOREK, A. and CIVIS, S. and KUBELIK, P. and FERUS, M. and ADAM,V. and VACULOVICOVA, M.},

doi = {https://doi.org/10.1016/j.microc.2021.106022},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Microchemical Journal.},

volume = {167.},

pages = {7},

publisher = {ACS Appl. Mater. Interfaces},

abstract = {Capillary electromigration techniques have proven their capabilities in detection of variety of analytes from inorganic ions and small organic molecules through bio(macro)molecules to large analytes such as cells or nano/micro particles. Also broad range of potential applications includes food and environmental analysis, biomedical and pharmaceutical investigations or even diagnostics.



In this work, it was demonstrated that capillary micellar electrokinetic chromatography is an excellent tool extremely helpful in investigations focused on prebiotic synthesis of molecules essential for formation of life on early Earth – nucleobases. In particular, rapid separation of nucleobases (< 2 min) was achieved in 40 mM borate buffer separation electrolyte containing 60 mM sodium dodecyl sulfate as an additive. This approach enabled detection of nucleobases formed in thermolysed formamide under conditions simulating the environment occurring on early Earth. Moreover, polydopamine-based molecularly imprinted polymers specific to thymine and uracil improved detection of these low abundant products.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32759824,

title = {UV-Induced Nanoparticles-Formation, Properties and Their Potential Role in Origin of Life},

author = {Lukas Nejdl and Kristyna Zemankova and Martina Havlikova and Michaela Buresova and David Hynek and Kledi Xhaxhiu and Filip Mravec and Martina Matouskova and Vojtech Adam and Martin Ferus and Jakub Kapus and Marketa Vaculovicova},

doi = {10.3390/nano10081529},

issn = {2079-4991},

year  = {2020},

date = {2020-08-01},

journal = {Nanomaterials (Basel)},

volume = {10},

number = {8},

abstract = {Inorganic nanoparticles might have played a vital role in the transition from inorganic chemistry to self-sustaining living systems. Such transition may have been triggered or controlled by processes requiring not only versatile catalysts but also suitable reaction surfaces. Here, experimental results showing that multicolor quantum dots might have been able to participate as catalysts in several specific and nonspecific reactions, relevant to the prebiotic chemistry are demonstrated. A very fast and easy UV-induced formation of ZnCd quantum dots (QDs) with a quantum yield of up to 47% was shown to occur 5 min after UV exposure of the solution containing Zn(II) and Cd(II) in the presence of a thiol capping agent. In addition to QDs formation, xanthine activity was observed in the solution. The role of solar radiation to induce ZnCd QDs formation was replicated during a stratospheric balloon flight.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32278983,

title = {Magnetic molecularly imprinted polymers used for selective isolation and detection of Staphylococcus aureus},

author = {Jaroslava Bezdekova and Kristyna Zemankova and Jitka Hutarova and Silvia Kociova and Kristyna Smerkova and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1016/j.foodchem.2020.126673},

issn = {1873-7072},

year  = {2020},

date = {2020-08-01},

journal = {Food Chem},

volume = {321},

pages = {126673},

abstract = {In this work, a novel method was developed, for isolation of S. aureus from complex (food) samples using molecular imprinting.  Dopamine was used as a functional monomer and fluorescence microscopy was used for detection. Conditions for preparation of molecularly imprinted polymers (MIPs), adsorption performance, adsorption kinetic, and selectivity of the polymeric layers were investigated. The various procedures were combined in a single extraction process, with the imprinted layer on the surface of the magnetic particles (magnetic MIPs). Subsequently, MIPs were used for extraction of S. aureus from milk and rice. Moreover, raw milk from cows with mastitis was tested successfully. Using this novel MIP-based method, it was possible to detect bacteria in milk at 1 × 10CFU·ml, which corresponds to the limit set in European Union legislation for microbial control of food.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32307169,

title = {Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk},

author = {Jaroslava Bezdekova and Marcela Vlcnovska and Kristyna Zemankova and Romana Bacova and Martina Kolackova and Tomas Lednicky and Jan Pribyl and Lukas Richtera and Lucie Vanickova and Vojtech Adam and Marketa Vaculovicova},

doi = {10.3168/jds.2019-17367},

issn = {1525-3198},

year  = {2020},

date = {2020-06-01},

journal = {J Dairy Sci},

volume = {103},

number = {6},

pages = {4941--4950},

abstract = {Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and eco-friendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32113552,

title = {Fully automated process for histamine detection based on magnetic separation and fluorescence detection},

author = {Milica Gagic and Lukas Nejdl and Kledi Xhaxhiu and Natalia Cernei and Ondrej Zitka and Ewelina Jamroz and Pavel Svec and Lukas Richtera and Pavel Kopel and Vedran Milosavljevic and Vojtech Adam},

doi = {10.1016/j.talanta.2020.120789},

issn = {1873-3573},

year  = {2020},

date = {2020-05-01},

journal = {Talanta},

volume = {212},

pages = {120789},

abstract = {To ensure food safety and to prevent unnecessary foodborne complications this study reports fast, fully automated process for histamine determination. This method is based on magnetic separation of histamine with magnetic particles and quantification by the fluorescence intensity change of MSA modified CdSe Quantum dots. Formation of FeO particles was followed by adsorption of TiO on their surface. Magnetism of developed probe enabled rapid histamine isolation prior to its fluorescence detection. Quantum dots (QDs) of approx. 3 nm were prepared via facile UV irradiation. The fluorescence intensity of CdSe QDs was enhanced upon mixing with magnetically separated histamine, in concentration-dependent manner, with a detection limit of 1.6 μM. The linear calibration curve ranged between 0.07 and 4.5 mM histamine with a low LOD and LOQ of 1.6 μM and 6 μM. The detection efficiency of the method was confirmed by ion exchange chromatography. Moreover, the specificity of the sensor was evaluated and no cross-reactivity from nontarget analytes was observed. This method was successfully applied for the direct analysis of histamine in white wine providing detection limit much lower than the histamine maximum levels established by EU regulation in food samples. The recovery rate was excellent, ranging from 84 to 100% with an RSD of less than 4.0%. The main advantage of the proposed method is full automation of the analytical procedure that reduces the time and cost of the analysis, solvent consumption and sample manipulation, enabling routine analysis of large numbers of samples for histamine and highly accurate and precise results.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid32290291,

title = {A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria},

author = {Vishma Pratap Sur and Aninda Mazumdar and Pavel Kopel and Soumajit Mukherjee and Petr Vítek and Hana Michalkova and Markéta Vaculovičová and Amitava Moulick},

doi = {10.3390/ijms21072656},

issn = {1422-0067},

year  = {2020},

date = {2020-04-01},

journal = {Int J Mol Sci},

volume = {21},

number = {7},

abstract = {The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against  (NCTC 8511), vancomycin-resistant  (VRSA) (CCM 1767), methicillin-resistant  (MRSA) (ST239: SCCmecIIIA), and hospital isolate . The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31706042,

title = {Application of molecularly imprinted polymers as artificial receptors for imaging},

author = {Tereza Vaneckova and Jaroslava Bezdekova and Gang Han and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1016/j.actbio.2019.11.007},

issn = {1878-7568},

year  = {2020},

date = {2020-01-01},

journal = {Acta Biomater},

volume = {101},

pages = {444--458},

abstract = {Medical diagnostics aims at specific localization of molecular targets as well as detection of abnormalities associated with numerous diseases. Molecularly imprinted polymers (MIPs) represent an approach of creating a synthetic material exhibiting selective recognition properties toward the desired template. The fabricated target-specific MIPs are usually well reproducible, economically efficient, and stable under critical conditions as compared to routinely used biorecognition elements such as fluorescent proteins, antibodies, enzymes, or aptamers and can even be created to those targets for which no antibodies are available. In this review, we summarize the methods of polymer fabrication. Further, we provide key for selection of the core material with imaging function depending on the imaging modality used. Finally, MIP-based imaging applications are highlighted and presented in a comprehensive form from different aspects. STATEMENT OF SIGNIFICANCE: In this review, we summarize the methods of polymer fabrication. Key applications of Molecularly imprinted polymers (MIPs) in imaging are highlighted and discussed with regard to the selection of the core material for imaging as well as commonly used imaging targets. MIPs represent an approach of creating a synthetic material exhibiting selective recognition properties toward the desired template. The fabricated target-specific MIPs are usually well reproducible, economically efficient, and stable under critical conditions as compared to routinely used biorecognition elements, e.g., antibodies, fluorescent proteins, enzymes, or aptamers, and can even be created to those targets for which no antibodies are available.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31996287,

title = {Direct Magnetic Bead-Based Extraction of MicroRNA from Urine with Capillary Electrophoretic Analysis Using Fluorescence Detection and Universal Label},

author = {Kristyna Smerkova and Tomas Rypar and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1166/jbn.2020.2872},

issn = {1550-7033},

year  = {2020},

date = {2020-01-01},

journal = {J Biomed Nanotechnol},

volume = {16},

number = {1},

pages = {76--84},

abstract = {Short non-coding RNAs, specifically microRNAs (miRNAs), are of a great interest due to their presumed function in genome regulation. Moreover, miRNAs are currently perceived as potential biomarkers for numerous diseases; a variety of detection methods and sensing systems have therefore been studied. We present a magnetic-bead-based assay for specific miRNA isolation coupled with sensitive electrophoretic analysis with fluorescence detection. The magnetic separation step involves creating a duplex with targeted miR-141, which is subsequently cleaved from the magnetic bead surface with a specific endonuclease. The duplex is then determined using capillary electrophoresis with laser-induced fluorescence detection in the presence of the fluorescent dye PicoGreen for quantitating double-stranded DNA. The benefits of using microcolumn separation technique coupled with sensitive detection over traditionally used determination by fluorescence spectrometry include the fact that there is no need for a specific pre-labeled fluorescent probe. This significantly simplifies the method and reduces the costs. Cross-reactivity with mismatched oligonucleotides (3 and 5 mismatched bases) and different miRNAs (miR-124 and miR-150) was tested, demonstrating the specificity of the developed method for miRNA-141. This magnetic extraction method was demonstrated for the direct isolation and determination of miR-141 at different concentration levels from urine samples and the achieved nanomolar detection limit.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine},

author = {Barbora Tesarova, and Simona Dostalova, and Veronika Smidova, and Zita Goliasova, and Zuzana Skubalova, and Hana Michalkova, and David Hynek, and Petr Michalek, and Hana Polanska, and Marketa Vaculovicova, and Jaromir Hacek, and Tomas Eckschlager, and Marie Stiborova, and Ana S. Pires, and Ana R.M. Neves, and Ana M. Abrantes, and Tiago Rodrigues, and Paulo Matafome, and Maria F. Botelho, and Paulo Teixeira, and Fernando Mendes, and Zbynek Heger.},

url = {https://doi.org/10.1016/j.apmt.2019.100501},

year  = {2019},

date = {2019-12-04},

urldate = {2019-12-04},

journal = {Applied Materials Today},

volume = {18},

abstract = {Surface functionalisations substantially influence the performance of drug delivery vehicles by improving their biocompatibility, selectivity and circulation in bloodstream. Herein, we present the study of in vitro and in vivo behaviour of a highly potent cytostatic alkaloid ellipticine (Elli) encapsulated in internal cavity of ferritin (FRT)-based nanocarrier (hereinafter referred to as FRTElli). In addition, FRTElli surface was functionalised with three different molecular coatings: two types of protective PAS peptides (10- or 20-residues lengths) with sequences comprising amino acids proline (P), alanine (A) and serine (S) (to form PAS-10-FRTElli or PAS-20-FRTElli, respectively), or polyethylene glycol (PEG-FRTElli). All three surface modifications of FRT disposed sufficient encapsulation efficiency of Elli with no premature cumulative release of cargo. Noteworthy, all tested surface modifications displayed beneficial effects on the in vitro biocompatibility. PAS-10-FRTElli exhibited markedly reduced uptake by macrophages compared to PAS-20-FRTElli, PEG-FRTElli or unmodified FRTElli. The exceptional properties of PAS-10-FRTElli were validated by an array of in vitro analyses including formation of protein corona, uptake efficiency or screenings of selectivity of cytotoxicity. In murine preclinical model bearing triple-negative breast cancer (MDA-MB-231) xenograft, compared to free Elli or FRTElli, PAS-10-FRTElli displayed enhanced accumulation of Elli within tumour tissue, while hampering the uptake of Elli into off-target tissues. Noteworthy, PAS-10-FRTElli led to decreased in vivo complement (C3) activation and protein corona formation. Taken together, presented in vivo results indicate that PAS-10-FRTElli represents a promising stealth platform for translation into clinical settings.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31417990,

title = {Prebiotic synthesis at impact craters: the role of Fe-clays and iron meteorites},

author = {Adam Pastorek and Jana Hrnčířová and Luboš Jankovič and Lukáš Nejdl and Svatopluk Civiš and Ondřej Ivanek and Violetta Shestivska and Antonín KníŽek and Petr Kubelík and Jiří Šponer and Lukáš Petera and Anna Křivková and Giuseppe Cassone and Markéta Vaculovičová and Judit E Šponer and Martin Ferus},

doi = {10.1039/c9cc04627e},

issn = {1364-548X},

year  = {2019},

date = {2019-08-01},

journal = {Chem Commun (Camb)},

volume = {55},

number = {71},

pages = {10563--10566},

abstract = {Besides delivering plausible prebiotic feedstock molecules and high-energy initiators, extraterrestrial impacts could also affect the process of abiogenesis by altering the early Earth's geological environment in which primitive life was conceived. We show that iron-rich smectites formed by reprocessing of basalts due to the residual post-impact heat could catalyze the synthesis and accumulation of important prebiotic building blocks such as nucleobases, amino acids and urea.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Versatile persistent luminescent oxycarbonates: Morphology evolution from nanorods through bamboo-like nanorods to nanoparticles},

author = {Xiaojing Dou, Yang Li and Tereza Vaneckova and Ru Kang and Yihua Hu and Hongli Wen and Xiuping Gao and Shaoan Zhang and Marketa Vaculovicova and Gang Han

},

doi = {https://doi.org/10.1016/j.jlumin.2019.116635},

issn = {0022-2313},

year  = {2019},

date = {2019-07-20},

journal = {Journal of Luminescence},

volume = {215},

abstract = {Nanomaterials with persistent luminescence (PLNMs) have emerged as important materials in autofluorescence-free detection for their exceptional ability to temporarily detach the excitation and emission procedure. Although significant advances in materials composition have been made, research of morphology-dependent PLNMs synthesis is still lacking. At its core, further understanding toward the morphology optimization and afterglow enhancement is needed for their future expansion. Herein, we report a two-step synthesis to prepare morphology and afterglow controllable persistent luminescent oxycarbonates (La2O2CO3: Eu3+, Ho3+). In step one, we emphasized a precipitation pretreatment to ensure uniform precursor nanorods. Then, a morphology evolution from nanorods through bamboo-like nanorods to nanoparticles as well as the control of afterglow time can be realized by simply changing posttreatment conditions, such as reaction duration and reaction power. In particular, we highlighted the nanoparticles can be easily produced via cutting the bamboo-like nanorod section by section during the effortless mechanical grinding. In addition, the as-obtained PLNMs exhibit an excellent aqueous stability and remain suspended in adtevak from live mouse and 10% glucose beyond 10 days. The experiment of autofluorescence-free H2O2 detection with La2O2CO3: Eu3+, Ho3+ PLNMs employed as sensing probe demonstrates that these PLNMs present a fresh way of using PLNMs as a new H2O2 detection mode without in situ excitation for real time monitoring of H2O2 and glucose.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid30876553,

title = {Molecularly imprinted polymers coupled to mass spectrometric detection for metallothionein sensing},

author = {Tereza Vaneckova and Lucie Vanickova and Michaela Tvrdonova and Adam Pomorski and Artur Krężel and Tomas Vaculovic and Viktor Kanicky and Marketa Vaculovicova and Vojtech Adam},

doi = {10.1016/j.talanta.2019.01.089},

issn = {1873-3573},

year  = {2019},

date = {2019-06-01},

journal = {Talanta},

volume = {198},

pages = {224--229},

abstract = {We report a facile method for detection of metallothionein (MT), a promising clinically relevant biomarker, in spiked plasma samples. This method, for the first time, integrates molecularly imprinted polymers as purification/pretreatment step with matrix assisted laser desorption/ionization time-of-flight mass spectrometric detection and with laser ablation inductively coupled plasma mass spectrometry for analysis of MTs. The prepared MT-imprinted polydopamine layer showed high binding capacity and specific recognition properties toward the template. Optimal monomer (dopamine) concentration was found to be 16 mM of dopamine. This experimental setup allows to measure µM concentrations of MT that are present in blood as this can be used for clinical studies recognizing MT as marker of various diseases including tumour one. Presented approach not only provides fast sample throughput but also avoids the limitations of methods based on use of antibodies (e.g. high price, cross-reactivity, limited availability in some cases, etc.).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31413275,

title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection},

author = {Tereza Vaneckova and Jaroslava Bezdekova and Michaela Tvrdonova and Marcela Vlcnovska and Veronika Novotna and Jan Neuman and Aneta Stossova and Viktor Kanicky and Vojtech Adam and Marketa Vaculovicova and Tomas Vaculovic},

doi = {10.1038/s41598-019-48290-2},

issn = {2045-2322},

year  = {2019},

date = {2019-01-01},

journal = {Sci Rep},

volume = {9},

number = {1},

pages = {11840},

abstract = {For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-antibody and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid30802685,

title = {Designing next generation of photon upconversion: Recent advances in organic triplet-triplet annihilation upconversion nanoparticles},

author = {Ling Huang and Eugenia Kakadiaris and Tereza Vaneckova and Kai Huang and Marketa Vaculovicova and Gang Han},

doi = {10.1016/j.biomaterials.2019.02.008},

issn = {1878-5905},

year  = {2019},

date = {2019-01-01},

journal = {Biomaterials},

volume = {201},

pages = {77--86},

abstract = {Organic triplet-triplet annihilation upconversion (TTA-UC) nanoparticles have emerged as exciting therapeutic agents and imaging probes in recent years due to their unique chemical and optical properties such as outstanding biocompatibility and low power excitation density. In this review, we focus on the latest breakthroughs in such new version of upconversion nanoparticle, including their design, preparation, and applications. First, we will discuss the key principles and design concept of these organic-based photon upconversion in regard to the methods of selection of the related triplet TTA dye pairs (photosensitizer and emitter). Then, we will discuss the recent approaches s to construct TTA-UCNPs including silica TTA-UCNPs, lipid-coated TTA-UCNPs, polymer encapsulated TTA-UCNPs, nano-droplet TTA-UCNPs and metal-organic frameworks (MOFs) constructed TTA-UCNPs. In addition, the applications of TTA-UCNPs will be discussed. Finally, we will discuss the challenges posed by current TTA-UCNP development.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid31226356,

title = {Nanoparticle-drug conjugates treating bacterial infections},

author = {Pavlina Jelinkova and Aninda Mazumdar and Vishma Pratap Sur and Silvia Kociova and Kristyna Dolezelikova and Ana Maria Jimenez Jimenez and Zuzana Koudelkova and Pawan Kumar Mishra and Kristyna Smerkova and Zbynek Heger and Marketa Vaculovicova and Amitava Moulick and Vojtech Adam},

doi = {10.1016/j.jconrel.2019.06.013},

issn = {1873-4995},

year  = {2019},

date = {2019-01-01},

journal = {J Control Release},

volume = {307},

pages = {166--185},

abstract = {The ever increasing scenario of bacterial resistance against commonly available antibiotics is becoming a global threat of major concern, which necessitates the development of new strategies to overcome this hurdle. Conjugation of nanoparticles (NPs) with antimicrobial moieties, such as antibiotics, peptides or different biomolecules, has been one of the successful techniques in targeting antibiotic resistance. This review mainly focusses on the possible nanoparticle-drug conjugates with their activity against pathogenic bacterial infections. Nanoparticles play an array of roles, e.g. as a carrier, synergistically acting agent and as theranostic agent, henceforth facilitates the efficacy of therapy. Moreover, this review elaborates the studies with reported nanoparticles-drug conjugates that include their possible synthesis methodologies and applications. In most of the cases, the nanoparticles were found to increase the permeability of bacterial cell membrane, which enables higher uptake of antibiotics inside the bacterial cells which in return showed better effects. Even the conjugates were found to efficiently kill the antibiotic-resistant strains. Since several limitations are exerted by the biological systems, there is an urge for the advancement of nanoparticle-drug conjugates for better proficiency.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid30109381,

title = {Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry},

author = {Michaela Tvrdonova and Marcela Vlcnovska and Lucie Pompeiano Vanickova and Viktor Kanicky and Vojtech Adam and Lena Ascher and Norbert Jakubowski and Marketa Vaculovicova and Tomas Vaculovic},

doi = {10.1007/s00216-018-1300-7},

issn = {1618-2650},

year  = {2019},

date = {2019-01-01},

journal = {Anal Bioanal Chem},

volume = {411},

number = {3},

pages = {559--564},

abstract = {In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Preparation of graphene quantum dots through liquid phase exfoliation method},

author = {L. Zdrazil and R. Zahradnicek and R. Mohan and P. Sedlacek and Lukas Nejdl and V. Schmiedova, J. Pospisil and M. Horak and M. Weiter and O. Zmeskal and J. Hubalek

},

doi = {https://doi.org/10.1016/j.jlumin.2018.08.017},

issn = {0022-2313},

year  = {2018},

date = {2018-08-07},

journal = {Journal of Luminescence},

volume = {204},

pages = {203-208},

abstract = {Here we report the preparation of low defect blue fluorescent graphene quantum dots (GQDs) through microwave expansion and liquid phase exfoliation (LPE) of graphite flakes with a high preparation yield of 0.63 g of GQDs per 1 g of original graphite substrate. The maximum emission in the prepared GQDs occurs at 467 nm for 370 nm excitation. The photoluminescence quantum yield of the prepared GQDs was measured as 3.4%. Prepared GQDs are composed of carbon, oxygen and nitrogen elements. They have high percentage (86%) of C-C/C=C, indicating few structural defects of the graphitic core, due to lesser number of edge- or surface-located oxygenated functional groups. This preparation technique, unlike others, does not require additional chemical components and is energetically undemanding. Furthermore, the reported method is also suitable for scalable preparation of quantum structures from other two-dimensional layered materials.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid29534794,

title = {Short-sweep capillary electrophoresis with a selective zinc fluorescence imaging reagent FluoZin-3 for determination of free and metalothionein-2a-bound Zn ions},

author = {Lukas Nejdl and Andrea Moravanska and Kristyna Smerkova and Filip Mravec and Sona Krizkova and Adam Pomorski and Artur Krężel and Mirek Macka and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1016/j.aca.2018.02.008},

issn = {1873-4324},

year  = {2018},

date = {2018-08-01},

journal = {Anal Chim Acta},

volume = {1017},

pages = {41--47},

abstract = {A capillary electrophoretic (CE) method using a short-sweep approach and laser-induced fluorescence (LIF) detection (ShortSweepCE-LIF) was developed for determination of Zn and Cd as complexes with highly selective and sensitive fluorescent probe FluoZin-3. The ShortSweepCE-LIF method, established in this work, can be used for examining competitive Zn and Cd binding properties of metalloproteins or peptides. The parameters including background electrolyte composition, injection pressure and time as well as separation voltage were investigated. Under the optimized conditions, 80 mM HEPES, pH 7.4, with 1.5 μM FluoZin-3 was used as an electrolyte, hydrodynamic injection was performed at 50 mbar for 5 s, and separation voltage of 25 kV. Limits of detection for Zn and Cd were 4 and 125 nM, respectively. The developed method was demonstrated in a study of interactions between metalothionein-2a isoform and metal ions Zn, Co and Cd. It was found that FluoZin-3 was able to extract a single Zn ion, while added Co (in surplus) extracted only 2.4 Zn ions, and Cd extracted all 7 Zn ions present in the metalothionein molecule.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid29384137,

title = {Capillary electrophoresis-driven synthesis of water-soluble CdTe quantum dots in nanoliter scale},

author = {Lukas Nejdl and David Hynek and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1088/1361-6528/aaabd4},

issn = {1361-6528},

year  = {2018},

date = {2018-04-01},

journal = {Nanotechnology},

volume = {29},

number = {16},

pages = {165602},

abstract = {'Green nanotechnology' is a term used for the design of nanomaterials and processes that reduce or eliminate the use and/or generation of hazardous substances. In this paper, a capillary electrophoresis (CE)-driven synthesis of CdTe quantum dots (QDs) and their subsequent conjugation with a metal-binding protein metallothionein (isofom MT1) is reported. Even though the toxic materials (cadmium and potassium borohydride) were used for synthesis, the proposed method can be labeled as 'environmentally friendly' because the whole process (synthesis of QDs and MT1 conjugation) was carried out under mild conditions: ultra-low volume (nanoliter scale), relatively low temperature (50 °C), atmospheric pressure, and completed in a short time (under 90 s). Prepared QDs were also characterized by classical fluorescence spectroscopy and transmission electron microscopy. This study opens up new possibilities for the utilization of classical CE in the synthesis of nanoparticles and on-line labeling of biomolecules in the nanoliter scale in short period of time.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Rapid preparation of self-assembled CdTe quantum dots used for sensing of DNA in urine},

author = {Nejdl, L. and Zelníčková, J. and Vanečková, T. and Hynek, D. and Adam, V. and Vaculovičová, M.},

doi = {10.1039/C7NJ05167K},

year  = {2018},

date = {2018-03-09},

journal = {New Journal of Chemistry},

issue = {8},

abstract = {In this article, the authors report a systematic study of the self-assembly of CdTe quantum dots (QDs) stabilized by mercaptosuccinic acid (MSA) at laboratory temperature (25 °C) or after thermal treatment (90 °C). It was found that QD/MSA can be synthesized spontaneously under mild laboratory conditions (25 °C and 70 hours) without any further instrumentation or they can be treated using an increased temperature (90 °C and 8 min) to achieve the same fluorescence intensity. These QDs were used for the preparation of a fluorescent (off–on) probe based on methylene blue as a quencher for the specific determination of nucleic acid from urine. The whole process was monitored using stationary spectroscopy (absorbance and fluorescence measurement) and capillary electrophoresis (CE) with laser-induced fluorescence detection. The average size of the prepared QDs (approximately 3 nm) was determined using transmission electron microscopy and it was proven that the presented fluorescent probe combined with the CE method can be used for the sensitive determination of DNA (LOD 3 ng mL−1).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid29882600,

title = {Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis},

author = {Tereza Vaneckova and Kristyna Smerkova and Jan Zitka and David Hynek and Ondrej Zitka and Antonin Hlavacek and Frantisek Foret and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1002/elps.201700483},

issn = {1522-2683},

year  = {2018},

date = {2018-01-01},

journal = {Electrophoresis},

volume = {39},

number = {17},

pages = {2246--2252},

abstract = {Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laser-induced fluorescence detection was used to investigate the behavior of carboxyl-silica-coated UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Peptide-mediated bioconjugation of antibodies to UCNPs was also investigated. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid29882600b,

title = {Upconversion nanoparticle bioconjugates characterized by capillary electrophoresis},

author = {Tereza Vaneckova and Kristyna Smerkova and Jan Zitka and David Hynek and Ondrej Zitka and Antonin Hlavacek and Frantisek Foret and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1002/elps.201700483},

issn = {1522-2683},

year  = {2018},

date = {2018-01-01},

journal = {Electrophoresis},

volume = {39},

number = {17},

pages = {2246--2252},

abstract = {Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laser-induced fluorescence detection was used to investigate the behavior of carboxyl-silica-coated UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Peptide-mediated bioconjugation of antibodies to UCNPs was also investigated. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28814513,

title = {Escape of Tick-Borne Flavivirus from 2'--Methylated Nucleoside Antivirals Is Mediated by a Single Conservative Mutation in NS5 That Has a Dramatic Effect on Viral Fitness},

author = {Ludek Eyer and Hirofumi Kondo and Darina Zouharova and Minato Hirano and James J Valdés and Memi Muto and Tomas Kastl and Shintaro Kobayashi and Jan Haviernik and Manabu Igarashi and Hiroaki Kariwa and Marketa Vaculovicova and Jiri Cerny and Rene Kizek and Andrea Kröger and Stefan Lienenklaus and Milan Dejmek and Radim Nencka and Martin Palus and Jiri Salat and Erik De Clercq and Kentaro Yoshii and Daniel Ruzek},

doi = {10.1128/JVI.01028-17},

issn = {1098-5514},

year  = {2017},

date = {2017-11-01},

journal = {J Virol},

volume = {91},

number = {21},

abstract = {Tick-borne encephalitis virus (TBEV) causes a severe and potentially fatal neuroinfection in humans. Despite its high medical relevance, no specific antiviral therapy is currently available. Here we demonstrate that treatment with a nucleoside analog, 7-deaza-2'--methyladenosine (7-deaza-2'-CMA), substantially improved disease outcomes, increased survival, and reduced signs of neuroinfection and viral titers in the brains of mice infected with a lethal dose of TBEV. To investigate the mechanism of action of 7-deaza-2'-CMA, two drug-resistant TBEV clones were generated and characterized. The two clones shared a signature amino acid substitution, S603T, in the viral NS5 RNA-dependent RNA polymerase (RdRp) domain. This mutation conferred resistance to various 2'--methylated nucleoside derivatives, but no cross-resistance was seen with other nucleoside analogs, such as 4'--azidocytidine and 2'-deoxy-2'-beta-hydroxy-4'-azidocytidine (RO-9187). All-atom molecular dynamics simulations revealed that the S603T RdRp mutant repels a water molecule that coordinates the position of a metal ion cofactor as 2'--methylated nucleoside analogs approach the active site. To investigate its phenotype, the S603T mutation was introduced into a recombinant TBEV strain (Oshima-IC) generated from an infectious cDNA clone and into a TBEV replicon that expresses a reporter luciferase gene (Oshima-REP-luc2A). The mutants were replication impaired, showing reduced growth and a small plaque size in mammalian cell culture and reduced levels of neuroinvasiveness and neurovirulence in rodent models. These results indicate that TBEV resistance to 2'--methylated nucleoside inhibitors is conferred by a single conservative mutation that causes a subtle atomic effect within the active site of the viral NS5 RdRp and is associated with strong attenuation of the virus. This study found that the nucleoside analog 7-deaza-2'--methyladenosine (7-deaza-2'-CMA) has high antiviral activity against tick-borne encephalitis virus (TBEV), a pathogen that causes severe human neuroinfections in large areas of Europe and Asia and for which there is currently no specific therapy. Treating mice infected with a lethal dose of TBEV with 7-deaza-2'-CMA resulted in significantly higher survival rates and reduced the severity of neurological signs of the disease. Thus, this compound shows promise for further development as an anti-TBEV drug. It is important to generate drug-resistant mutants to understand how the drug works and to develop guidelines for patient treatment. We generated TBEV mutants that were resistant not only to 7-deaza-2'-CMA but also to a broad range of other 2'--methylated antiviral medications. Our findings suggest that combination therapy may be used to improve treatment and reduce the emergence of drug-resistant viruses during nucleoside analog therapy for TBEV infection.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28792458,

title = {Amalgam Electrode-Based Electrochemical Detector for On-Site Direct Determination of Cadmium(II) and Lead(II) from Soils},

author = {Lukas Nejdl and Jindrich Kynicky and Martin Brtnicky and Marketa Vaculovicova and Vojtech Adam},

doi = {10.3390/s17081835},

issn = {1424-8220},

year  = {2017},

date = {2017-08-01},

journal = {Sensors (Basel)},

volume = {17},

number = {8},

abstract = {Toxic metal contamination of the environment is a global issue. In this paper, we present a low-cost and rapid production of amalgam electrodes used for determination of Cd(II) and Pb(II) in environmental samples (soils and wastewaters) by on-site analysis using difference pulse voltammetry. Changes in the electrochemical signals were recorded with a miniaturized potentiostat (width: 80 mm, depth: 54 mm, height: 23 mm) and a portable computer. The limit of detection (LOD) was calculated for the geometric surface of the working electrode 15 mm² that can be varied as required for analysis. The LODs were 80 ng·mL for Cd(II) and 50 ng·mL for Pb(II), relative standard deviation, RSD ≤ 8% ( = 3). The area of interest (Dolni Rozinka, Czech Republic) was selected because there is a deposit of uranium ore and extreme anthropogenic activity. Environmental samples were taken directly on-site and immediately analysed. Duration of a single analysis was approximately two minutes. The average concentrations of Cd(II) and Pb(II) in this area were below the global average. The obtained values were verified (correlated) by standard electrochemical methods based on hanging drop electrodes and were in good agreement. The advantages of this method are its cost and time effectivity (approximately two minutes per one sample) with direct analysis of turbid samples (soil leach) in a 2 M HNO₃ environment. This type of sample cannot be analyzed using the classical analytical methods without pretreatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Nanotechnology-based analytical approaches for detection of viruses},

author = {Vaculovičová, M. and Michálek, P. and Křížková, S. and Macka, M. and Adam, V. },

doi = {10.1039/C7AY00048K},

year  = {2017},

date = {2017-04-01},

journal = {Analytical Methods},

issue = {16},

abstract = {The risks posed by viral diseases have been recognised as critical threats to mankind. On the other hand, using nanotechnology approaches, viruses are used as tools and building blocks for electronics, chemistry, and biomedical science. Virus-based nanotechnology is benefiting from the natural circulatory and targeting properties of viruses in order to design drugs and vaccines that specifically target tissues of interest in vivo. This review details the exploitation of nanomaterials for the detection of viruses in general, with a focus on four of the most common and damaging viruses, namely influenza, hepatitis, HIV, and dengue. As the principles behind the techniques used in the area of detection and analysis are interconnected with the principles used in some other areas, the review also outlines the use of viral nanoparticles as nanocarriers for targeted delivery as well as their utilization in nanomaterial engineering.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28446250,

title = {Nanoparticles based on essential metals and their phytotoxicity},

author = {Branislav Ruttkay-Nedecky and Olga Krystofova and Lukas Nejdl and Vojtech Adam},

doi = {10.1186/s12951-017-0268-3},

issn = {1477-3155},

year  = {2017},

date = {2017-04-01},

journal = {J Nanobiotechnology},

volume = {15},

number = {1},

pages = {33},

abstract = {Nanomaterials in agriculture are becoming popular due to the impressive advantages of these particles. However, their bioavailability and toxicity are key features for their massive employment. Herein, we comprehensively summarize the latest findings on the phytotoxicity of nanomaterial products based on essential metals used in plant protection. The metal nanoparticles (NPs) synthesized from essential metals belong to the most commonly manufactured types of nanomaterials since they have unique physical and chemical properties and are used in agricultural and biotechnological applications, which are discussed. The paper discusses the interactions of nanomaterials and vascular plants, which are the subject of intensive research because plants closely interact with soil, water, and atmosphere; they are also part of the food chain. Regarding the accumulation of NPs in the plant body, their quantification and localization is still very unclear and further research in this area is necessary.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Real-time monitoring of the UV-induced formation of quantum dots on a milliliter, microliter, and nanoliter scale},

author = {Lukas Nejdl and Jan Zitka and Filip Mravec and Vedran Milosavljevic and Ondrej Zitka and Pavel Kopel and Vojtech Adam and Marketa Vaculovicova},

doi = {10.1007/s00604-017-2149-8},

year  = {2017},

date = {2017-03-03},

journal = {Microchimica Acta},

pages = {1489–1497},

abstract = {The authors report on a systematic study on the low-cost, low-temperature, and fast synthesis of water soluble quantum dots (QDs) stabilized by mercaptosuccinic acid by UV irradiation. The effects of UV irradiation (at 254 nm and 250 nm) and temperature on the precursors (Cd:Se, Cd:Te, Cd, Zn:S, Zn:Se and Zn) are described. Best results are achieved with a mixture of precursors containing cadmium, selenium and MSA where a 10-min irradiation with 254-nm light gives CdSe QDs with a quantum yield of 13.5%. The authors also describe the preparation and monitoring of the formation of QDs in sub-mg, sub-μg and sub-ng quantities, the smallest concentration being 258 pg in volume of 4 nL. The growth of the QDs can be monitored in real time by absorption, fluorescence and dynamic light scattering. The solutions of the particles also are characterized by fluorescence correlation spectroscopy and detected by LED-induced fluorescence. The preparation of such QDs by UV radiation is simple, easily controllable, and inexpensive. Conceivably, it can be integrated with lab-on-chip, micro total analysis systems or other instrumentation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28203652,

title = {Nanomaterials for sample pretreatment prior to capillary electrophoretic analysis},

author = {Vojtech Adam and Marketa Vaculovicova},

doi = {10.1039/c6an02608g},

issn = {1364-5528},

year  = {2017},

date = {2017-03-01},

journal = {Analyst},

volume = {142},

number = {6},

pages = {849--857},

abstract = {Nanomaterials are, in analytical science, used for a broad range of purposes, covering the area of sample pretreatment as well as separation, detection and identification of target molecules. This review covers the application of nanomaterials for sample pretreatment in capillary electrophoresis. It targets the utilization of nanomaterials for sample purification, preconcentration and/or extraction coupled both off-line and on-line with capillary electrophoretic analysis. Especially due to their large surface area, nanoparticles and nanomaterials are exceptionally helpful in making up for the limited concentration detection limits provided by capillary electrophoresis. This method possesses excellent separation power; however, its sensitivity may be problematic in some cases. Therefore, this review is focused on utilization of nanomaterials as a powerful tool for sample preconcentration, which is so often required prior to capillary electrophoretic analysis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid27894780,

title = {Antibody-free detection of infectious bacteria using quantum dots-based barcode assay},

author = {Kristyna Cihalova and Dagmar Hegerova and Ana Maria Jimenez and Vedran Milosavljevic and Jiri Kudr and Sylvie Skalickova and David Hynek and Pavel Kopel and Marketa Vaculovicova and Vojtech Adam},

doi = {10.1016/j.jpba.2016.10.025},

issn = {1873-264X},

year  = {2017},

date = {2017-02-01},

journal = {J Pharm Biomed Anal},

volume = {134},

pages = {325--332},

abstract = {Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the most representative bacteria causing infectious diseases. Due to the increased application of antibiotics, the bacterial resistance is growing causing severe complications. Therefore, a sensitive determination of these pathogens is crucial for effective treatment. The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from properties of magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detect as low concentrations of bacteria as 10 CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28704436,

title = {Platinum nanoparticles induce damage to DNA and inhibit DNA replication},

author = {Lukas Nejdl and Jiri Kudr and Amitava Moulick and Dagmar Hegerova and Branislav Ruttkay-Nedecky and Jaromir Gumulec and Kristyna Cihalova and Kristyna Smerkova and Simona Dostalova and Sona Krizkova and Marie Novotna and Pavel Kopel and Vojtech Adam},

doi = {10.1371/journal.pone.0180798},

issn = {1932-6203},

year  = {2017},

date = {2017-01-01},

journal = {PLoS One},

volume = {12},

number = {7},

pages = {e0180798},

abstract = {Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28665525,

title = {Capillary electrophoresis and nanomaterials - Part I: Capillary electrophoresis of nanomaterials},

author = {Vojtech Adam and Marketa Vaculovicova},

doi = {10.1002/elps.201700097},

issn = {1522-2683},

year  = {2017},

date = {2017-01-01},

journal = {Electrophoresis},

volume = {38},

number = {19},

pages = {2389--2404},

abstract = {Nanomaterials are in analytical science used for a broad range of purposes, covering the area of sample pretreatment as well as separation, detection, and identification of target molecules. This part of the review covers capillary electrophoresis (CE) of nanomaterials and focuses on the application of CE as a method for characterization used during nanomaterial synthesis and modification as well as the monitoring of their properties and interactions with other molecules. The heterogeneity of the nanomaterial family is extremely large. Depending on different definitions of the term Nanomaterial/Nanoparticle, the group may cover metal and polymeric nanoparticles, carbon nanomaterials, liposomes and even dendrimers. Moreover, these nanomaterials are usually subjected to some kind of surface modification or functionalization, which broadens the diversity even more. Not only for purposes of verification of nanomaterial synthesis and batch-to-batch quality check, but also for determination the polydispersity and for functionality characterization on the nanoparticle surface, has CE offered very beneficial capabilities. Finally, the monitoring of interactions between nanomaterials and other (bio)molecules is easily performed by some kind of capillary electromigration technique.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28703399,

title = {CE and nanomaterials - Part II: Nanomaterials in CE},

author = {Vojtech Adam and Marketa Vaculovicova},

doi = {10.1002/elps.201700098},

issn = {1522-2683},

year  = {2017},

date = {2017-01-01},

journal = {Electrophoresis},

volume = {38},

number = {19},

pages = {2405--2430},

abstract = {The scope of this two-part review is to summarize publications dealing with CE and nanomaterials together. This topic can be viewed from two broad perspectives, and this article is trying to highlight these two approaches: (i) CE of nanomaterials, and (ii) nanomaterials in CE. The second part aims at summarization of publications dealing with application of nanomaterials for enhancement of CE performance either in terms of increasing the separation resolution or for improvement of the detection. To increase the resolution, nanomaterials are employed as either surface modification of the capillary wall forming open tubular column or as additives to the separation electrolyte resulting in a pseudostationary phase. Moreover, nanomaterials have proven to be very beneficial for increasing also the sensitivity of detection employed in CE or even they enable the detection (e.g., fluorescent tags of nonfluorescent molecules).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28392686,

title = {Apoferritin as an ubiquitous nanocarrier with excellent shelf life},

author = {Simona Dostalova and Katerina Vasickova and David Hynek and Sona Krizkova and Lukas Richtera and Marketa Vaculovicova and Tomas Eckschlager and Marie Stiborova and Zbynek Heger and Vojtech Adam},

doi = {10.2147/IJN.S130267},

issn = {1178-2013},

year  = {2017},

date = {2017-01-01},

journal = {Int J Nanomedicine},

volume = {12},

pages = {2265--2278},

abstract = {Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated the long-term stability of the naturally found protein nanocarrier apoferritin (Apo) with encapsulated doxorubicin (Dox). The encapsulation was performed using Apo's ability to disassemble reversibly into its subunits at low pH (2.7) and reassemble in neutral pH (7.2), physically entrapping drug molecules in its cavity (creating ApoDox). In this study, ApoDox was prepared in water and phosphate-buffered saline and stored for 12 weeks in various conditions (-20°C, 4°C, 20°C, and 37°C in dark, and 4°C and 20°C under ambient light). During storage, a very low amount of prematurely released drug molecules were detected (maximum of 7.5% for ApoDox prepared in PBS and 4.4% for ApoDox prepared in water). Fourier-transform infrared spectra revealed no significant differences in any of the samples after storage. Most of the ApoDox prepared in phosphate-buffered saline and ApoDox prepared in water and stored at -20°C formed very large aggregates (up to 487% of original size). Only ApoDox prepared in water and stored at 4°C showed no significant increase in size or shape. Although this storage caused slower internalization to LNCaP prostate cancer cells, ApoDox (2.5 μM of Dox) still retained its ability to inhibit completely the growth of 1.5×10 LNCaP cells after 72 hours. ApoDox stored at 20°C and 37°C in water was not able to deliver Dox inside the nucleus, and thus did not inhibit the growth of the LNCaP cells. Overall, our study demonstrates that ApoDox has very good stability over the course of 12 weeks when stored properly (at 4°C), and is thus suitable for use as a nanocarrier in the specific delivery of anticancer drugs to patients.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28059036,

title = {Reduction of Doxorubicin-Induced Cardiotoxicity Using Nanocarriers: A Review},

author = {Michaela Fojtu and Jaromir Gumulec and Tibor Stracina and Martina Raudenska and Anna Skotakova and Marketa Vaculovicova and Vojtech Adam and Petr Babula and Marie Novakova and Michal Masarik},

doi = {10.2174/1389200218666170105165444},

issn = {1875-5453},

year  = {2017},

date = {2017-01-01},

journal = {Curr Drug Metab},

volume = {18},

number = {3},

pages = {237--263},

abstract = {BACKGROUND: Anthracycline antibiotic doxorubicin (DOX) is a very potent and extensively prescribed chemotherapeutic drug. It is widely utilized in the therapy of variety of haematological and solid tumours, although its administration is commonly accompanied with several severe side effects. The most serious one is a development of dose-dependent and cumulative cardiotoxicity. In the course of time, many strategies have been investigated in order to avoid or at least to diminish DOX-induced cardiac dysfunction; these include reduction of toxic effect by coadministration with iron chelators (dexrazoxane), trastuzumab, taxanes, statins, and ACE-inhibitors. However, the attenuation of cardiotoxic effect is still not satisfactory yet.



OBJECTIVE: This review represents an overall appraisal of studies concerning with the utilization of various doxorubicinloaded nanoparticles in the cancer treatment with specific emphasis on those studies evaluating their influence on the reduction of heart tissue damage.



CONCLUSION: Introduction of nanoscale drug delivery systems undoubtedly represents nowadays one of the most promising tools for lowering systemic toxicity. Nanoparticles enable to target the therapeutic payload directly towards the tumor tissue, thus leading to the increased accumulation of the drug in the desired tissue and simultaneously protecting surrounding healthy tissues.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28970072,

title = {DNA interaction with platinum-based cytostatics revealed by DNA sequencing},

author = {Kristyna Smerkova and Tomas Vaculovic and Marketa Vaculovicova and Jindrich Kynicky and Martin Brtnicky and Tomas Eckschlager and Marie Stiborova and Jaromir Hubalek and Vojtech Adam},

doi = {10.1016/j.ab.2017.09.018},

issn = {1096-0309},

year  = {2017},

date = {2017-01-01},

journal = {Anal Biochem},

volume = {539},

pages = {22--28},

abstract = {The main mechanism of action of platinum-based cytostatic drugs - cisplatin, oxaliplatin and carboplatin - is the formation of DNA cross-links, which restricts the transcription due to the disability of DNA to enter the active site of the polymerase. The polymerase chain reaction (PCR) was employed as a simplified model of the amplification process in the cell nucleus. PCR with fluorescently labelled dideoxynucleotides commonly employed for DNA sequencing was used to monitor the effect of platinum-based cytostatics on DNA in terms of decrease in labeling efficiency dependent on a presence of the DNA-drug cross-link. It was found that significantly different amounts of the drugs - cisplatin (0.21 μg/mL), oxaliplatin (5.23 μg/mL), and carboplatin (71.11 μg/mL) - were required to cause the same quenching effect (50%) on the fluorescent labelling of 50 μg/mL of DNA. Moreover, it was found that even though the amounts of the drugs was applied to the reaction mixture differing by several orders of magnitude, the amount of incorporated platinum, quantified by inductively coupled plasma mass spectrometry, was in all cases at the level of tenths of μg per 5 μg of DNA.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid27219717,

title = {Site-Directed Conjugation of Antibodies to Apoferritin Nanocarrier for Targeted Drug Delivery to Prostate Cancer Cells},

author = {Simona Dostalova and Tereza Cerna and David Hynek and Zuzana Koudelkova and Tomas Vaculovic and Pavel Kopel and Jan Hrabeta and Zbynek Heger and Marketa Vaculovicova and Tomas Eckschlager and Marie Stiborova and Vojtech Adam},

doi = {10.1021/acsami.6b04286},

issn = {1944-8252},

year  = {2016},

date = {2016-06-01},

journal = {ACS Appl Mater Interfaces},

volume = {8},

number = {23},

pages = {14430--14441},

abstract = {Herein, we describe a novel approach for targeting of ubiquitous protein apoferritin (APO)-encapsulating doxorubicin (DOX) to prostate cancer using antibodies against prostate-specific membrane antigen (PSMA). The conjugation of anti-PSMA antibodies and APO was carried out using HWRGWVC heptapeptide, providing their site-directed orientation. The prostate-cancer-targeted and nontargeted nanocarriers were tested using LNCaP and HUVEC cell lines. A total of 90% of LNCaP cells died after treatment with DOX (0.25 μM) or DOX in nontargeted and prostate-cancer-targeted APO, proving that the encapsulated DOX toxicity for LNCaP cells remained the same. Free DOX showed higher toxicity for nonmalignant cells, whereas the toxicity was lower after treatment with the same dosage of APO-encapsulated DOX (APODOX) and even more in prostate-cancer-targeted APODOX. Hemolytic assay revealed exceptional hemocompatibility of the entire nanocarrier. The APO encapsulation mechanism ensures applicability using a wide variety of chemotherapeutic drugs, and the presented surface modification enables targeting to various tumors. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase},

author = {Zbynek Heger and Jan Zitka and Lukas Nejdl and Amitava Moulick and Vedran Milosavljevic and Pavel Kopel and Ondrej Zavodsky and Jakub Kapus and Libor Lenza and Milan Rezka and Vojtech Adam and Rene Kizek},

doi = {10.1007/s00706-016-1705-y},

year  = {2016},

date = {2016-03-10},

urldate = {2016-03-10},

journal = {Monatshefte für Chemie - Chemical Monthly},

pages = {873–880},

abstract = {We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (λ = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile–butadiene–styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (λ exc = 245 nm, λ em = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than −70 °C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Antiviral activity of fullerene C60 nanocrystals modified with derivatives of anionic antimicrobial peptide maximin H5},

author = {Simona Dostalova and Amitava Moulick and Vedran Milosavljevic and Roman Guran and Marketa Kominkova and Kristyna Cihalova and Zbynek Heger and Lucie Blazkova and Pavel Kopel and David Hynek and Marketa Vaculovicova and Vojtech Adam and Rene Kizek},

doi = {10.1007/s00706-016-1675-0},

year  = {2016},

date = {2016-03-01},

journal = {Monatshefte fuer Chemie/Chemical Monthly },

abstract = {Many active antiviral substances come from natural sources. In this way, peptides, isolated from Asian toad Bombina maxima, called maximins, are very promising. Most of them have good antimicrobial activity; however, derivatives of anionic 20 amino acids-long maximin H5 show also promising antiviral activity. The effect can be enhanced by binding to suitable nanocarriers such as fullerenes. In the present study, six mutants of maximin H5 were designed where aspartic acid at position 11 was replaced by asparagine, histidine, tyrosine, alanine, glycine, or valine. The binding yield of each peptide to fullerene C60 nanocrystals was studied by derivatization with fluorescent reagent fluorescamine. The antiviral activity of these peptides and peptides bound to fullerene C60 nanocrystals was studied using bacteriophage λ as a model virus. All of the designed peptides had higher antiviral activity compared to maximin H5. The highest antiviral activity was observed in case of maximin variants H5N, H5V, or H5Y. Moreover, the antiviral activity was dependent on the amount of peptide bound on the surface of fullerene C60 nanocrystals, which was enhanced by trimesic acid (benzene-1,3,5-tricarboxylic acid) treated fullerene C60 nanocrystals.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26462605,

title = {Bioconjugation of peptides using advanced nanomaterials to examine their interactions in 3D printed flow-through device},

author = {Petr Michalek and Lukas Richtera and Ludmila Krejcova and Lukas Nejdl and Renata Kensova and Jan Zitka and Pavel Kopel and Zbynek Heger and Vojtech Adam and Rene Kizek},

doi = {10.1002/elps.201500345},

issn = {1522-2683},

year  = {2016},

date = {2016-02-01},

journal = {Electrophoresis},

volume = {37},

number = {3},

pages = {444--454},

abstract = {Peptide-peptide interactions are crucial in the living cell as they lead to the formation of the numerous types of complexes. In this study, synthetic peptides containing 11 of cysteines (α-domain of metallothionein (MT)) and sialic acid binding region (130-loop of hemagglutinin (HA)) were employed. The aim of the experiment was studying the interactions between MT and HA-derived peptides. For this purpose, fragments were tagged with cysteines at C-terminal part to serve as ligand sites for PbS and CuS quantum dots (QDs), and therefore these conjugates can be traced and quantified during wide spectrum of methods. As a platform for interaction, γ-Fe2O3 paramagnetic particles modified with tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane (hydrodynamic diameter 30-40 nm) were utilized and MT/HA interactions were examined using multi-instrumental approach including electrochemistry, electrophoretic methods, and MALDI-TOF/TOF mass spectrometry. It was found that peptides enter mutual creation of complexes, which are based on some of nonbonded interactions. The higher willingness to interact was observed in MT-derived peptides toward immobilized HA. Finally, we designed and manufactured flow-through electrochemical 3D printed device (reservoir volume 150 μL) and utilized it for automated analysis of the HA/MT metal labels. Under the optimal conditions, (deposition time and flow rate 80 s and 1.6 mL/min for CuS and 120 s and 1.6 mL/min PbS, respectively), the results of peptide-conjugated QDs were comparable with atomic absorption spectrometry.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26927112,

title = {Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)},

author = {Sylvie Skalickova and Lukas Nejdl and Jiri Kudr and Branislav Ruttkay-Nedecky and Ana Maria Jimenez Jimenez and Pavel Kopel and Monika Kremplova and Michal Masarik and Marie Stiborova and Tomas Eckschlager and Vojtech Adam and Rene Kizek},

doi = {10.3390/s16030290},

issn = {1424-8220},

year  = {2016},

date = {2016-02-01},

journal = {Sensors (Basel)},

volume = {16},

number = {3},

pages = {290},

abstract = {Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid28787832,

title = {Improved Electrochemical Detection of Zinc Ions Using Electrode Modified with Electrochemically Reduced Graphene Oxide},

author = {Jiri Kudr and Lukas Richtera and Lukas Nejdl and Kledi Xhaxhiu and Petr Vitek and Branislav Rutkay-Nedecky and David Hynek and Pavel Kopel and Vojtech Adam and Rene Kizek},

doi = {10.3390/ma9010031},

issn = {1996-1944},

year  = {2016},

date = {2016-01-01},

journal = {Materials (Basel)},

volume = {9},

number = {1},

abstract = {Increasing urbanization and industrialization lead to the release of metals into the biosphere, which has become a serious issue for public health. In this paper, the direct electrochemical reduction of zinc ions is studied using electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE). The graphene oxide (GO) was fabricated using modified Hummers method and was electrochemically reduced on the surface of GCE by performing cyclic voltammograms from 0 to -1.5 V. The modification was optimized and properties of electrodes were determined using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The determination of Zn(II) was performed using differential pulse voltammetry technique, platinum wire as a counter electrode, and Ag/AgCl/3 M KCl reference electrode. Compared to the bare GCE the modified GCE/ERGO shows three times better electrocatalytic activity towards zinc ions, with an increase of reduction current along with a negative shift of reduction potential. Using GCE/ERGO detection limit 5 ng·mL was obtained.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26188679,

title = {Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application},

author = {Maja Stanisavljevic and Sona Krizkova and Marketa Vaculovicova and Rene Kizek and Vojtech Adam},

doi = {10.1016/j.bios.2015.06.076},

issn = {1873-4235},

year  = {2015},

date = {2015-12-01},

journal = {Biosens Bioelectron},

volume = {74},

pages = {562--574},

abstract = {Fluorescence resonance energy transfer (FRET) in combination with quantum dots (QDs) and their superior properties has enabled designing of the new and improved sensors. In this review, the latest novelties in development and application of FRET nanosensors employing QDs are presented. QDs offer several advantages over organic dyes - broad excitation spectra, narrow defined tunable emission peak, longer fluorescence lifetime, resistance to photobleaching and 10-100 times higher molar extinction coefficient. These properties of QDs allow multicolor QDs to be excited from one source by common fluorescent dyes without emission signal overlap and results in brighter probes comparing to conventional fluorophores. Due to these benefits, QD-FRET-based nanosensors gained a wide spread popularity in a variety of scientific areas. These sensors are most frequently applied in the domain of the nucleic acid and enzyme activity detection. Other applications are detection of peptides and low-molecular compounds, environmental pollutants, viruses, microorganisms and their toxins, QD-FRET-based immunoassays, and pH sensors. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pmid26188679b,

title = {Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application},

author = {Maja Stanisavljevic and Sona Krizkova and Marketa Vaculovicova and Rene Kizek and Vojtech Adam},

doi = {10.1016/j.bios.2015.06.076},

issn = {1873-4235},

year  = {2015},

date = {2015-12-01},

journal = {Biosens Bioelectron},

volume = {74},

pages = {562--574},

abstract = {Fluorescence resonance energy transfer (FRET) in combination with quantum dots (QDs) and their superior properties has enabled designing of the new and improved sensors. In this review, the latest novelties in development and application of FRET nanosensors employing QDs are presented. QDs offer several advantages over organic dyes - broad excitation spectra, narrow defined tunable emission peak, longer fluorescence lifetime, resistance to photobleaching and 10-100 times higher molar extinction coefficient. These properties of QDs allow multicolor QDs to be excited from one source by common fluorescent dyes without emission signal overlap and results in brighter probes comparing to conventional fluorophores. Due to these benefits, QD-FRET-based nanosensors gained a wide spread popularity in a variety of scientific areas. These sensors are most frequently applied in the domain of the nucleic acid and enzyme activity detection. Other applications are detection of peptides and low-molecular compounds, environmental pollutants, viruses, microorganisms and their toxins, QD-FRET-based immunoassays, and pH sensors. },

keywords = {},

pubstate = {published},

tppubtype = {article}

}